Q2RH96 (PCKA2_MOOTA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoenolpyruvate carboxykinase [ATP] 2
Short name=PEP carboxykinase 2
Short name=PEPCK
EC=4.1.1.49

Alternative name(s):
Phosphoenolpyruvate carboxylase 2

Gene names

Name:	pckA2
Ordered Locus Names:	Moth_1893

Organism

Moorella thermoacetica (strain ATCC 39073)

Taxonomic identifier

264732 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacteriaceae › Moorella group › Moorella

Protein attributes

Sequence length	526 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2. HAMAP MF_00453
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_00453
Subcellular location	Cytoplasm By similarity HAMAP MF_00453.
Sequence similarities	Belongs to the phosphoenolpyruvate carboxykinase [ATP] family.

Ontologies

Keywords
Biological process	Gluconeogenesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoenolpyruvate carboxykinase (ATP) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 526	526	Phosphoenolpyruvate carboxykinase [ATP] 2 HAMAP MF_00453		PRO_0000236929
Regions
	Nucleotide binding	231 – 238	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2RH96 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: 716B87DEC89D300A	FASTA	526	57,522
	10 20 30 40 50 60 MGNTYGLEGL GLINPGNIYR NLPVARLVEM ALARGEGVLA SNGALSVRTG KYTGRSPNDR 70 80 90 100 110 120 FMVDTPSVHD TISWGAVNQP LEEARFEALF RRLAAYLQGR DLFIFDGFVG ADPAYRMPIR 130 140 150 160 170 180 VVNQYAWQNL FVHQLFVRPS AAELEAHEPQ FTVICAPGFQ ATPEVDGTRS EAFVVLNFDR 190 200 210 220 230 240 RLIIIGGTSY AGEMKKSIFT VMNYLLPERG VCPMHCSANM GPAGDTALFF GLSGTGKTTL 250 260 270 280 290 300 SADPERRLIG DDEHGWSDHG IFNFEGGCYA KCIKLSAEHE PQIWNAIRFG SVLENVAVDP 310 320 330 340 350 360 DSRAIDYDCD ALTENTRAAY PVDFIPNAVI PGIGGHPRTV VFLTADAFGV MPPIAKLTRE 370 380 390 400 410 420 QAMYHFLSGY TSKLAGTERG ITDPQATFST CFGAPFLPRS PRVYADLLGE RIAKHGASVY 430 440 450 460 470 480 LVNTGWTGGP SGIGRRMSLP YTRAMVRAAI KGELEGVEFV PDPVFGILVP TSCPGVPAEV 490 500 510 520 LNPRHTWQDK EKYDAMARKL AGLFAENFTK FRDVPEEIRA AGPVVR

References

[1]

"The complete genome sequence of Moorella thermoacetica (f. Clostridium thermoaceticum)." Pierce E., Xie G., Barabote R.D., Saunders E., Han C.S., Detter J.C., Richardson P., Brettin T.S., Das A., Ljungdahl L.G., Ragsdale S.W. Environ. Microbiol. 10:2550-2573(2008) [PubMed: 18631365] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39073.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000232 Genomic DNA. Translation: ABC20193.1.
RefSeq	YP_430736.1. NC_007644.1.
3D structure databases
ProteinModelPortal	Q2RH96.
SMR	Q2RH96. Positions 6-525.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2RH96.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3831166.
GenomeReviews	Gene locus Moth_1893 in contig CP000232_GR.
KEGG	mta:Moth_1893.
NMPDR	fig|264732.9.peg.1899.
PATRIC	22641164. VBIMooThe6753_2050.
Phylogenomic databases
eggNOG	COG1866.
HOGENOM	HBG293387.
OMA	HEKANNE.
PhylomeDB	Q2RH96.
ProtClustDB	PRK09344.
Enzyme and pathway databases
BioCyc	MTHE264732:MOTH_1893-MONOMER.
Family and domain databases
HAMAP	MF_00453. PEPCK_ATP. [Tree]
InterPro	IPR001272. PEP_carboxykinase_ATP. IPR013035. PEP_carboxykinase_C. IPR008210. PEP_carboxykinase_N. IPR015994. PEPCK_ATP_CS. [Graphical view]
Gene3D	G3DSA:3.90.228.20. PEP_carboxykinase_C. 2 hits. G3DSA:3.40.449.10. PEP_carboxykinase_N. 1 hit.
KO	K01610.
Pfam	PF01293. PEPCK_ATP. 1 hit. [Graphical view]
PIRSF	PIRSF006294. PEP_crbxkin. 1 hit.
SUPFAM	SSF68923. PEP_carboxykinase_N. 1 hit.
TIGRFAMs	TIGR00224. PckA. 1 hit.
PROSITE	PS00532. PEPCK_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PCKA2_MOOTA

Accession

Primary (citable) accession number: Q2RH96

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	January 24, 2006
Last modified:	January 25, 2012
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program