Reviewed, UniProtKB/Swiss-Prot Q2RGX6 (THIM_MOOTA)

Names and origin

Protein names

Recommended name:
    Hydroxyethylthiazole kinase
    EC=2.7.1.50
Alternative name(s):
    4-methyl-5-beta-hydroxyethylthiazole kinase
      Short name=Thz kinase
      Short name=TH kinase

Gene names

Name:	thiM
Ordered Locus Names:	Moth_2017

Organism

Moorella thermoacetica (strain ATCC 39073) [Complete proteome] [HAMAP]

Taxonomic identifier

264732 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacteriaceae › Moorella group › Moorella

Protein attributes

Sequence length	267 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP MF_00228
Pathway	Cofactor biosynthesis; thiamine pyrophosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 4-methyl-5-(2-hydroxyethyl)-thiazole: step 1/1. HAMAP MF_00228
Sequence similarities	Belongs to the Thz kinase family.

Ontologies

Keywords
Biological process	Thiamine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	thiamin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP hydroxyethylthiazole kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 267	267	Hydroxyethylthiazole kinase HAMAP MF_00228		PRO_1000021523
Sites
	Binding site	46	1	Substrate; via amide nitrogen By similarity
	Binding site	122	1	ATP By similarity
	Binding site	168	1	ATP By similarity
	Binding site	195	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2RGX6-1 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: FDAEF4164D4F0BBD	FASTA	267	27,779
	10 20 30 40 50 60 MNWGEQVASR RQRVHEQRPL VHHITNLVVT NLTANVTLAI GASPVMAYAR EEVADMARLA 70 80 90 100 110 120 QAVVLNMGTL TGDVVEAMLL AGKAANNAGI PVVFDPVGAG ATPFRTRTAQ KIIKELHIDI 130 140 150 160 170 180 LRGNASEIAS IGGFGGHTKG VDAAGAPARV AEMVKQVARD LNTVVAVTGA TDYISDGERL 190 200 210 220 230 240 IAVDNGHPLL TFVTGTGCSA TSIIAAFRAV EEDGVTASTA ALAYYGLAAE RAAAVSQGPA 250 260 SFQVALLDTL YNLAGEELAR GVKIRLL

References

[1]

"The complete genome sequence of Moorella thermoacetica (f. Clostridium thermoaceticum)." Pierce E., Xie G., Barabote R.D., Saunders E., Han C.S., Detter J.C., Richardson P., Brettin T.S., Das A., Ljungdahl L.G., Ragsdale S.W. Environ. Microbiol. 10:2550-2573(2008) [PubMed: 18631365] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000232 Genomic DNA. Translation: ABC20313.1.
RefSeq	YP_430856.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3831971.
GenomeReviews	Gene locus Moth_2017 in contig CP000232_GR.
KEGG	mta:Moth_2017.
NMPDR	fig|264732.9.peg.2034.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2RGX6.
OMA	Q2RGX6. ASPVMAH.
Enzyme and pathway databases
BioCyc	MTHE264732:MOTH_2017-MON.
Family and domain databases
HAMAP	MF_00228. [Tree]
InterPro	IPR000417. Hyethyz_kinase. IPR011144. Hyethyz_kinsmonf. [Graphical view]
PANTHER	PTHR20857:SF14. Hyethyz_kinase. 1 hit.
Pfam	PF02110. HK. 1 hit. [Graphical view]
PIRSF	PIRSF000513. Thz_kinase. 1 hit.
PRINTS	PR01099. HYETHTZKNASE.
TIGRFAMs	TIGR00694. thiM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

THIM_MOOTA

Accession

Primary (citable) accession number: Q2RGX6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 24, 2006
Last modified:	June 16, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)