Q2RGU7 (PUR5_MOOTA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine cyclo-ligase
EC=6.3.3.1

Alternative name(s):
AIR synthase
AIRS
Phosphoribosyl-aminoimidazole synthetase

Gene names

Name:	purM
Ordered Locus Names:	Moth_2046

Organism

Moorella thermoacetica (strain ATCC 39073) [Reference proteome] [HAMAP]

Taxonomic identifier

264732 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacteraceae › Moorella group › Moorella › Moorella thermoacetica

Protein attributes

Sequence length	350 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. HAMAP-Rule MF_00741
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. HAMAP-Rule MF_00741
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the AIR synthase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine cyclo-ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 350	350	Phosphoribosylformylglycinamidine cyclo-ligase HAMAP-Rule MF_00741		PRO_0000258371

Sequences

	Sequence		Length	Mass (Da)
	Q2RGU7 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: 8FD81506D016C349	FASTA	350	37,591
	10 20 30 40 50 60 MVAGEGFTYA AAGVDIAAGN RAVELMKEHV RRTFRPGVLG DLGGFGGLFA LEAGRYRQPV 70 80 90 100 110 120 LVAGTDGVGT KLKIAFSLDR HDTIGIDAVA MCVNDILVQG AEPLFFLDYL AVGKMVPERV 130 140 150 160 170 180 ARIVAGVAEG CRRAGCALIG GETAEMPGFY REDEYDLAGF AVGVVEREEL LDGSRIRPGQ 190 200 210 220 230 240 VVLGLASSGL HSNGFSLARR VLLVEAGYTL ERKLPELGRT LGEELLEPTR IYVASILPLL 250 260 270 280 290 300 KEGLIKGLAH ITGGGLIENP PRILPPGCSL RLDRRSWPVP PAFRLIQATG RVPEEEMYRT 310 320 330 340 350 FNMGLGMLVV VEESDAGRVK SRLEAAGEKV FVVGEVIPGR REVEFVPGLQ

References

[1]

"The complete genome sequence of Moorella thermoacetica (f. Clostridium thermoaceticum)." Pierce E., Xie G., Barabote R.D., Saunders E., Han C.S., Detter J.C., Richardson P., Brettin T.S., Das A., Ljungdahl L.G., Ragsdale S.W. Environ. Microbiol. 10:2550-2573(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39073.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000232 Genomic DNA. Translation: ABC20342.1.
RefSeq	YP_430885.1. NC_007644.1.
3D structure databases
ProteinModelPortal	Q2RGU7.
SMR	Q2RGU7. Positions 21-345.
ModBase	Search...
Protein-protein interaction databases
STRING	264732.Moth_2046.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABC20342; ABC20342; Moth_2046.
GeneID	3831192.
KEGG	mta:Moth_2046.
PATRIC	22641508. VBIMooThe6753_2222.
Phylogenomic databases
eggNOG	COG0150.
HOGENOM	HOG000229091.
KO	K01933.
OMA	IDMIAMN.
ProtClustDB	PRK05385.
Enzyme and pathway databases
BioCyc	MTHE264732:GH0A-2137-MONOMER.
UniPathway	UPA00074; UER00129.
Family and domain databases
HAMAP	MF_00741_B. AIRS_B.
InterPro	IPR010918. AIR_synth_C_dom. IPR000728. AIR_synth_N_dom. IPR004733. PurM_cligase. IPR016188. PurM_N-like. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 1 hit. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 1 hit. SSF55326. PurM_N-like. 1 hit.
TIGRFAMs	TIGR00878. purM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR5_MOOTA

Accession

Primary (citable) accession number: Q2RGU7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	January 24, 2006
Last modified:	May 1, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program