Q2RFM0 (ISPD_MOOTA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
EC=2.7.7.60

Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase
MEP cytidylyltransferase
Short name=MCT

Gene names

Name:	ispD
Ordered Locus Names:	Moth_2487

Organism

Moorella thermoacetica (strain ATCC 39073)

Taxonomic identifier

264732 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacteriaceae › Moorella group › Moorella

Protein attributes

Sequence length	229 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00108
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108
Sequence similarities	Belongs to the IspD family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 229	229	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_00108		PRO_0000237798
Sites
	Site	16	1	Transition state stabilizer By similarity
	Site	23	1	Transition state stabilizer By similarity
	Site	155	1	Positions MEP for the nucleophilic attack By similarity
	Site	211	1	Positions MEP for the nucleophilic attack By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2RFM0 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: 19F96B11994D156A	FASTA	229	24,102
	10 20 30 40 50 60 MPFLSMIIAA AGRGRRLGRE VNKVFLPLAG KPMLAYSLAV AVASPLVDEI IIVTRPDDIP 70 80 90 100 110 120 LCRQVVAGEG AGKVKAIVAG GAERQDSIAE GLKAVARAAE WVAVHDGARP FLTPALLEKT 130 140 150 160 170 180 ILAARNTGAA IAALPVKETI KQGGEAGIVA ATLDRKGLWS AQTPQVFRRE WLVDAYREAA 190 200 210 220 EKGWQATDDA ALVERLGYPV KLVPGEETNI KITTPGDLVL AGALLAGEK

References

[1]

"The complete genome sequence of Moorella thermoacetica (f. Clostridium thermoaceticum)." Pierce E., Xie G., Barabote R.D., Saunders E., Han C.S., Detter J.C., Richardson P., Brettin T.S., Das A., Ljungdahl L.G., Ragsdale S.W. Environ. Microbiol. 10:2550-2573(2008) [PubMed: 18631365] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39073.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000232 Genomic DNA. Translation: ABC20769.1.
RefSeq	YP_431312.1. NC_007644.1.
3D structure databases
ProteinModelPortal	Q2RFM0.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2RFM0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3831590.
GenomeReviews	Gene locus Moth_2487 in contig CP000232_GR.
KEGG	mta:Moth_2487.
NMPDR	fig|264732.9.peg.2518.
PATRIC	22642492. VBIMooThe6753_2707.
Phylogenomic databases
eggNOG	COG1211.
HOGENOM	HBG672839.
OMA	SKVIVVC.
PhylomeDB	Q2RFM0.
Enzyme and pathway databases
BioCyc	MTHE264732:MOTH_2487-MONOMER.
Family and domain databases
HAMAP	MF_00108. IspD. [Tree]
InterPro	IPR001228. ISPD_synthase. [Graphical view]
KO	K00991.
Pfam	PF01128. IspD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00453. IspD. 1 hit.
PROSITE	PS01295. ISPD. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISPD_MOOTA

Accession

Primary (citable) accession number: Q2RFM0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	January 24, 2006
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program