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Protein

6-phosphogluconate dehydrogenase, decarboxylating 2, chloroplastic

Gene

G6PGH2

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO2, with concomitant reduction of NADP to NADPH.By similarity

Catalytic activityi

6-phospho-D-gluconate + NADP+ = D-ribulose 5-phosphate + CO2 + NADPH.

Pathwayi: pentose phosphate pathway

This protein is involved in step 3 of the subpathway that synthesizes D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage).
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glucose-6-phosphate 1-dehydrogenase (LOC_Os03g29950), Glucose-6-phosphate 1-dehydrogenase (OsJ_23916), Glucose-6-phosphate 1-dehydrogenase (P0613B07.138), Glucose-6-phosphate 1-dehydrogenase (Os02g0600400), Glucose-6-phosphate 1-dehydrogenase (pG6PDH), Glucose-6-phosphate 1-dehydrogenase (LOC_Os03g20300), Glucose-6-phosphate 1-dehydrogenase (Os04g0485300), Glucose-6-phosphate 1-dehydrogenase (LOC_Os03g29950), Glucose-6-phosphate 1-dehydrogenase (g6pdh)
  2. Probable 6-phosphogluconolactonase 1 (Os03g0416500), Probable 6-phosphogluconolactonase 4, chloroplastic (Os09g0529100), Probable 6-phosphogluconolactonase 3, chloroplastic (Os08g0547100), Probable 6-phosphogluconolactonase 2 (Os07g0604000)
  3. 6-phosphogluconate dehydrogenase, decarboxylating 2, chloroplastic (G6PGH2), 6-phosphogluconate dehydrogenase, decarboxylating (P0029D06.14-2), 6-phosphogluconate dehydrogenase, decarboxylating (Os06g0111500), 6-phosphogluconate dehydrogenase, decarboxylating (OsJ_33909), 6-phosphogluconate dehydrogenase, decarboxylating 1 (G6PGH1)
This subpathway is part of the pathway pentose phosphate pathway, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage), the pathway pentose phosphate pathway and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei123NADPBy similarity1
Binding sitei123SubstrateBy similarity1
Active sitei203Proton acceptorBy similarity1
Active sitei210Proton donorBy similarity1
Binding sitei211SubstrateBy similarity1
Binding sitei284Substrate; via amide nitrogenBy similarity1
Binding sitei311SubstrateBy similarity1
Binding sitei475Substrate; shared with dimeric partnerBy similarity1
Binding sitei481Substrate; shared with dimeric partnerBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi28 – 33NADPBy similarity6
Nucleotide bindingi51 – 53NADPBy similarity3
Nucleotide bindingi95 – 97NADPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processGluconate utilization, Pentose shunt
LigandNADP

Enzyme and pathway databases

ReactomeiR-OSA-71336. Pentose phosphate pathway (hexose monophosphate shunt).
UniPathwayiUPA00115; UER00410.

Names & Taxonomyi

Protein namesi
Recommended name:
6-phosphogluconate dehydrogenase, decarboxylating 2, chloroplastic (EC:1.1.1.44)
Short name:
OsG6PGH2
Gene namesi
Name:G6PGH2
Ordered Locus Names:Os11g0484500, LOC_Os11g29400
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000059680 Componenti: Chromosome 11

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 12ChloroplastSequence analysisAdd BLAST12
ChainiPRO_000042110213 – 5086-phosphogluconate dehydrogenase, decarboxylating 2, chloroplasticAdd BLAST496

Proteomic databases

PaxDbiQ2R480.
PRIDEiQ2R480.

Expressioni

Inductioni

By drought, cold, high salinity and abscisic acid (ABA) treatments.1 Publication

Gene expression databases

ExpressionAtlasiQ2R480. baseline and differential.
GenevisibleiQ2R480. OS.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi39947.LOC_Os11g29400.1.

Structurei

3D structure databases

ProteinModelPortaliQ2R480.
SMRiQ2R480.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni149 – 151Substrate bindingBy similarity3
Regioni206 – 207Substrate bindingBy similarity2

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2653. Eukaryota.
COG0362. LUCA.
HOGENOMiHOG000255147.
InParanoidiQ2R480.
KOiK00033.
OMAiQALYMGK.
OrthoDBiEOG09360AB8.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
InterProiView protein in InterPro
IPR008927. 6-PGluconate_DH-like_C_sf.
IPR013328. 6PGD_dom2.
IPR006114. 6PGDH_C.
IPR006113. 6PGDH_Gnd/GntZ.
IPR006115. 6PGDH_NADP-bd.
IPR036291. NAD(P)-bd_dom_sf.
IPR006183. Pgluconate_DH.
PfamiView protein in Pfam
PF00393. 6PGD. 1 hit.
PF03446. NAD_binding_2. 1 hit.
PIRSFiPIRSF000109. 6PGD. 1 hit.
PRINTSiPR00076. 6PGDHDRGNASE.
SMARTiView protein in SMART
SM01350. 6PGD. 1 hit.
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00873. gnd. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q2R480-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASPAPAPPA ASSSAAGSAP PPRIGLAGLA TMGQNLALNI AEKGFPISVY
60 70 80 90 100
NRTAAKVDAT VSRAEAEGAL PVLGHRDPRG FVLSLSRPRT VVLLVQAGRA
110 120 130 140 150
VDATIDALVP YLDAGDAIVD GGNEWYQNTE RRIEEAAARG ILYLGMGVSG
160 170 180 190 200
GEEGARNGPS LMPGGHIDAY NNIRDILEKA AAQTEDGACV TFVGPGGAGN
210 220 230 240 250
FVKMVHNGIE YGDMQLIAEA YDVLRRVGGL SNSEIADVFA EWNRGELESF
260 270 280 290 300
LVEITADIFT VADPLDGSGG GGLVDKILDK TGMKGTGKWT VQQAAELAIA
310 320 330 340 350
APTIAASLDG RYLSGLKDER VAAAGVLEAE GMPSGLLETI NVDKKMLVDR
360 370 380 390 400
VRQALYASKI CSYAQGMNLL RAKSVEKGWN LNLAELARIW KGGCIIRAKF
410 420 430 440 450
LDRIKKAYDR NPELANLIVD REFAREMVQR QNAWRWVVAR AVEAGISTPG
460 470 480 490 500
MSASLSYFDT YRCSRLPANL IQAQRDLFGA HTYERIDRPG SFHTEWTKLA

RKSNGAAI
Length:508
Mass (Da):54,300
Last modified:January 24, 2006 - v1
Checksum:iFBF1FAB42278D49B
GO

Sequence cautioni

The sequence AAP33506 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti35N → K in AAP33506 (Ref. 6) Curated1
Sequence conflicti328E → K in AAP33506 (Ref. 6) Curated1
Sequence conflicti503S → T in AK071592 (PubMed:12869764).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DP000010 Genomic DNA. Translation: ABA93694.1.
AP008217 Genomic DNA. Translation: BAF28275.1.
AP014967 Genomic DNA. Translation: BAT14060.1.
AK071592 mRNA. No translation available.
AY278362 mRNA. Translation: AAP33506.2. Different initiation.
RefSeqiXP_015616899.1. XM_015761413.1.
UniGeneiOs.91715.

Genome annotation databases

EnsemblPlantsiOS11T0484500-01; OS11T0484500-01; OS11G0484500.
GeneIDi4350528.
GrameneiOS11T0484500-01; OS11T0484500-01; OS11G0484500.
KEGGiosa:4350528.

Similar proteinsi

Entry informationi

Entry namei6PGD2_ORYSJ
AccessioniPrimary (citable) accession number: Q2R480
Secondary accession number(s): A0A0P0Y2I7, Q7Y248
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 6, 2013
Last sequence update: January 24, 2006
Last modified: November 22, 2017
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families