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Q2R3A1 (CHS1_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chalcone synthase 1

Short name=OsCHS1
EC=2.3.1.74
Alternative name(s):
Naregenin-chalcone synthase
Gene names
Name:CHS1
Synonyms:CHS
Ordered Locus Names:Os11g0530600, LOC_Os11g32650
ORF Names:OsJ_032788
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length398 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin.

Catalytic activity

3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2.

Pathway

Secondary metabolite biosynthesis; flavonoid biosynthesis.

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the chalcone/stilbene synthases family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 398398Chalcone synthase 1
PRO_0000216017

Regions

Region58 – 658Coenzyme A binding By similarity
Region219 – 2202Substrate binding By similarity

Sites

Active site1671Acyl-thioester intermediate By similarity
Binding site2001Substrate By similarity
Binding site3111Coenzyme A; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2R3A1 [UniParc].

Last modified January 24, 2006. Version 1.
Checksum: FD5D8C28FE60FAE3

FASTA39843,265
        10         20         30         40         50         60 
MAAAVTVEEV RRAQRAEGPA TVLAIGTATP ANCVYQADYP DYYFRITKSE HMVELKEKFK 

        70         80         90        100        110        120 
RMCDKSQIRK RYMHLTEEIL QENPNMCAYM APSLDARQDI VVVEVPKLGK AAAQKAIKEW 

       130        140        150        160        170        180 
GQPRSRITHL VFCTTSGVDM PGADYQLAKM LGLRPNVNRL MMYQQGCFAG GTVLRVAKDL 

       190        200        210        220        230        240 
AENNRGARVL AVCSEITAVT FRGPSESHLD SMVGQALFGD GAAAVIVGSD PDEAVERPLF 

       250        260        270        280        290        300 
QMVSASQTIL PDSEGAIDGH LREVGLTFHL LKDVPGLISK NIERALGDAF TPLGISDWNS 

       310        320        330        340        350        360 
IFWVAHPGGP AILDQVEAKV GLDKERMRAT RHVLSEYGNM SSACVLFILD EMRKRSAEDG 

       370        380        390 
HATTGEGMDW GVLFGFGPGL TVETVVLHSV PITAGAAA 

« Hide

References

« Hide 'large scale' references
[1]"Sequence analysis and some characterization of single-copied chalcone synthase from rice."
Minami E., He D., Minami N., Shibuya N.
Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Nipponbare.
Tissue: Leaf.
[2]"Molecular cloning and characterization of chalcone synthase (CHS) gene from rice (Oryza sativa L)."
Ichikawa H., Okano E., Shimizu T., Nakamura S., Kawasaki S., Minami E.
Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Nipponbare.
[3]"The sequence of rice chromosomes 11 and 12, rich in disease resistance genes and recent gene duplications."
The rice chromosomes 11 and 12 sequencing consortia
BMC Biol. 3:20-20(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[4]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[5]"The rice annotation project database (RAP-DB): 2008 update."
The rice annotation project (RAP)
Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: cv. Nipponbare.
[6]"The genomes of Oryza sativa: a history of duplications."
Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. expand/collapse author list , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB000801 mRNA. Translation: BAA19186.2.
AB058397 Genomic DNA. Translation: BAB39764.1.
DP000010 Genomic DNA. Translation: ABA94123.1.
AP008217 Genomic DNA. Translation: BAF28372.1.
CM000148 Genomic DNA. Translation: EAZ18579.1.
RefSeqNP_001068009.1. NM_001074541.1.
UniGeneOs.12416.

3D structure databases

ProteinModelPortalQ2R3A1.
SMRQ2R3A1. Positions 6-392.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ2R3A1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsOS11T0530600-01; OS11T0530600-01; OS11G0530600.
GeneID4350636.
KEGGosa:4350636.

Organism-specific databases

GrameneQ2R3A1.

Phylogenomic databases

eggNOGCOG3424.
KOK00660.
OMAEDGHATT.
ProtClustDBPLN03170.

Enzyme and pathway databases

UniPathwayUPA00154.

Family and domain databases

Gene3D3.40.47.10. 2 hits.
InterProIPR012328. Chalcone/stilbene_synth_C.
IPR018088. Chalcone/stilbene_synthase_AS.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011141. Polyketide_synthase_type-III.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamPF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
[Graphical view]
PIRSFPIRSF000451. PKS_III. 1 hit.
SUPFAMSSF53901. SSF53901. 2 hits.
PROSITEPS00441. CHALCONE_SYNTH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCHS1_ORYSJ
AccessionPrimary (citable) accession number: Q2R3A1
Secondary accession number(s): P48405, P93710, Q9AVC2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: January 24, 2006
Last modified: April 16, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

PATHWAY comments

Index of metabolic and biosynthesis pathways