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Protein

CBL-interacting protein kinase 32

Gene

CIPK32

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

CIPK serine-threonine protein kinases interact with CBL proteins. Binding of a CBL protein to the regulatory NAF domain of CIPK protein lead to the activation of the kinase in a calcium-dependent manner (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mn2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei42 – 421ATPPROSITE-ProRule annotation
Active sitei136 – 1361Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi19 – 279ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Manganese, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
CBL-interacting protein kinase 32 (EC:2.7.11.1)
Alternative name(s):
OsCIPK32
Gene namesi
Name:CIPK32
Ordered Locus Names:Os12g0132200, LOC_Os12g03810
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
Proteomesi
  • UP000059680 Componenti: Chromosome 12, cultivar: Nipponbare

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 438438CBL-interacting protein kinase 32PRO_0000338389Add
BLAST

Proteomic databases

PaxDbiQ2QY53.

Expressioni

Gene expression databases

ExpressionAtlasiQ2QY53. baseline.
GenevisibleiQ2QY53. OS.

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os12g03810.2.

Structurei

3D structure databases

ProteinModelPortaliQ2QY53.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini13 – 268256Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini305 – 32925NAFPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni154 – 18330Activation loopBy similarityAdd
BLAST
Regioni335 – 36430PPIBy similarityAdd
BLAST

Domaini

The activation loop within the kinase domain is the target of phosphorylation/activation by upstream protein kinases. The PPI motif mediates the interaction with the ABI (abscisic acid-insensitive) phosphatases (By similarity).By similarity

Sequence similaritiesi

Contains 1 NAF domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0583. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000233016.
InParanoidiQ2QY53.

Family and domain databases

Gene3Di3.30.310.80. 1 hit.
InterProiIPR028375. KA1/Ssp2_C.
IPR011009. Kinase-like_dom.
IPR018451. NAF/FISL_domain.
IPR004041. NAF_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF03822. NAF. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF103243. SSF103243. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50816. NAF. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2QY53-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTTKVKRRV GKYELGRTIG EGTFAKVKFA RDTETGDPVA IKILDKEKVL
60 70 80 90 100
KHKMVEQIKR EISTMKLIKH PNVVRIYEVM GSKTKIYIVL EYVTGGELFD
110 120 130 140 150
TIVNHGRMRE DEARRYFQQL INAVDYCHSR GVYHRDLKPE NLLLDSYGNL
160 170 180 190 200
KVSDFGLSAL SQQIKDDGLL HTTCGTPNYV APEVLEDQGY DGAMADLWSC
210 220 230 240 250
GVILFVLLAG YLPFEDSNLM TLYKKISNAE FTFPPWTSFP AKRLLTRILD
260 270 280 290 300
PNPMTRVTIP EILEDEWFKK GYKRPEFDEK YDTTLDDVYA VFNDSEEHHV
310 320 330 340 350
TEKKEEPEAL NAFELISMSA GLNLGNLFDS EQEFKRETRF TSKCPPKEIV
360 370 380 390 400
RKIEEAAKPL GFDVQKKNYK LRLEKVKAGR KGNLNVATEI LQVAPSLHMV
410 420 430
EVRKAKGDTL EFHKFYKNLS RTLKDVVWKS DDLQNQLS
Length:438
Mass (Da):50,338
Last modified:July 11, 2006 - v2
Checksum:iB023935447DE97CF
GO

Sequence cautioni

The sequence AK111675 differs from that shown. Reason: Frameshift at position 256. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DP000011 Genomic DNA. Translation: ABA95716.2.
AP008218 Genomic DNA. Translation: BAF29098.1.
AP014968 Genomic DNA. No translation available.
AK111675 mRNA. No translation available.
RefSeqiXP_015619859.1. XM_015764373.1.
XP_015619860.1. XM_015764374.1.
UniGeneiOs.10274.

Genome annotation databases

GeneIDi4351419.
KEGGiosa:4351419.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DP000011 Genomic DNA. Translation: ABA95716.2.
AP008218 Genomic DNA. Translation: BAF29098.1.
AP014968 Genomic DNA. No translation available.
AK111675 mRNA. No translation available.
RefSeqiXP_015619859.1. XM_015764373.1.
XP_015619860.1. XM_015764374.1.
UniGeneiOs.10274.

3D structure databases

ProteinModelPortaliQ2QY53.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39947.LOC_Os12g03810.2.

Proteomic databases

PaxDbiQ2QY53.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi4351419.
KEGGiosa:4351419.

Phylogenomic databases

eggNOGiKOG0583. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000233016.
InParanoidiQ2QY53.

Gene expression databases

ExpressionAtlasiQ2QY53. baseline.
GenevisibleiQ2QY53. OS.

Family and domain databases

Gene3Di3.30.310.80. 1 hit.
InterProiIPR028375. KA1/Ssp2_C.
IPR011009. Kinase-like_dom.
IPR018451. NAF/FISL_domain.
IPR004041. NAF_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF03822. NAF. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF103243. SSF103243. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50816. NAF. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The sequence of rice chromosomes 11 and 12, rich in disease resistance genes and recent gene duplications."
    The rice chromosomes 11 and 12 sequencing consortia
    BMC Biol. 3:20-20(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  4. Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  5. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Nipponbare.
  6. "Calcium sensors and their interacting protein kinases: genomics of the Arabidopsis and rice CBL-CIPK signaling networks."
    Kolukisaoglu U., Weinl S., Blazevic D., Batistic O., Kudla J.
    Plant Physiol. 134:43-58(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiCIPKW_ORYSJ
AccessioniPrimary (citable) accession number: Q2QY53
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: July 11, 2006
Last modified: May 11, 2016
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.