Q2QWT4 (DPNP_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3'(2'),5'-bisphosphate nucleotidase
EC=3.1.3.7

Alternative name(s):
3'(2'),5-bisphosphonucleoside 3'(2')-phosphohydrolase
DPNPase

Gene names

Ordered Locus Names:

Os12g0183300, LOC_Os12g08280

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	358 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Converts adenosine 3'-phosphate 5'-phosphosulfate (PAPS) to adenosine 5'-phosphosulfate (APS) and 3'(2')-phosphoadenosine 5'- phosphate (PAP) to AMP. Regulates the flux of sulfur in the sulfur-activation pathway by converting PAPS to APS By similarity.
Catalytic activity	Adenosine 3',5'-bisphosphate + H2O = adenosine 5'-phosphate + phosphate.
Cofactor	Magnesium By similarity.
Enzyme regulation	Inhibited by Ca2+, Li+, and Na+ and activated by K+ By similarity.
Sequence similarities	Belongs to the inositol monophosphatase family.

Ontologies

Keywords
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	sulfur compound metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	3'(2'),5'-bisphosphate nucleotidase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 358	358	3'(2'),5'-bisphosphate nucleotidase		PRO_0000142535
Regions
	Region	142 – 145	4	Substrate binding By similarity
Sites
	Metal binding	78	1	Magnesium 1 By similarity
	Metal binding	140	1	Magnesium 1 By similarity
	Metal binding	140	1	Magnesium 2 By similarity
	Metal binding	142	1	Magnesium 1; via carbonyl oxygen By similarity
	Metal binding	143	1	Magnesium 2 By similarity
	Metal binding	292	1	Magnesium 2 By similarity
	Binding site	78	1	Substrate By similarity
	Binding site	292	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2QWT4 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: EFE813C18BEDB5FD	FASTA	358	37,480
	10 20 30 40 50 60 MSQAAGNPYA AELAAAKKAV TLAARLCQAV QKDILQSGVQ SKADQSPVTV ADYGSQILVS 70 80 90 100 110 120 LVLKMEAPAS SSFSMVAEED SEELRKEGAE EILENITELV NETIVDDGTY SIYFSKEGIL 130 140 150 160 170 180 SAIDDGKSEG GPSGRHWVLD PIDGTKGFLR GDQYAIALAL LDEGKVVLGV LACPNLSLGS 190 200 210 220 230 240 IGNLNGGSSG DQVGALFSAT IGCGAEVESL QGSPAQKISV CSIDNPVEAS FFESYEGAHS 250 260 270 280 290 300 LRDLTGSIAE KLGVQAPPVR IDSQAKYGAL ARGDGAIYLR FPHKGYREKI WDHAAGSIVV 310 320 330 340 350 TEAGGLVTDA SGNDLDFSKG RFLDLDTGII ATNKQLMPSL LKAVQDAIKE QNQAASPL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The sequence of rice chromosomes 11 and 12, rich in disease resistance genes and recent gene duplications." The rice chromosomes 11 and 12 sequencing consortia BMC Biol. 3:20-20(2005) [PubMed: 16188032] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed: 18089549] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 159-358. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DP000011 Genomic DNA. Translation: ABA95973.1. AP008218 Genomic DNA. Translation: BAF29345.1. AK058937 mRNA. No translation available.
RefSeq	NP_001066326.1. NM_001072858.1.
UniGene	Os.37640.
3D structure databases
ProteinModelPortal	Q2QWT4.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2QWT4.
Proteomic databases
PRIDE	Q2QWT4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os12g08280.1; LOC_Os12g08280.1; LOC_Os12g08280.
GeneID	4351686.
KEGG	osa:4351686.
Organism-specific databases
Gramene	Q2QWT4.
Phylogenomic databases
GeneTree	EPGT00070000029602.
HOGENOM	HBG599916.
OMA	HAAGCIV.
PhylomeDB	Q2QWT4.
ProtClustDB	CLSN2698784.
Family and domain databases
InterPro	IPR006239. Bisphos_HAL2. IPR020583. Inositol_monoP_metal-BS. IPR000760. Inositol_monophosphatase. IPR020550. Inositol_monophosphatase_CS. [Graphical view]
KO	K15422.
PANTHER	PTHR20854. Inositol_P. 1 hit.
Pfam	PF00459. Inositol_P. 1 hit. [Graphical view]
PRINTS	PR00377. IMPHPHTASES.
TIGRFAMs	TIGR01330. Bisphos_HAL2. 1 hit.
PROSITE	PS00629. IMP_1. 1 hit. PS00630. IMP_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DPNP_ORYSJ

Accession

Primary (citable) accession number: Q2QWT4
Secondary accession number(s): Q40639

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 24, 2007
Last sequence update:	January 24, 2006
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program