Reviewed, UniProtKB/Swiss-Prot Q2QUN2 (LAC24_ORYSJ)

Names and origin

Protein names

Recommended name:
    Laccase-24
    EC=1.10.3.2
Alternative name(s):
    Benzenediol:oxygen oxidoreductase 24
    Urishiol oxidase 24
    Diphenol oxidase 24

Gene names

Name:	LAC24
Ordered Locus Names:	Os12g0258700, LOC_Os12g15680
ORF Names:	OsJ_016843

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	579 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Lignin degradation and detoxification of lignin-derived products By similarity.
Catalytic activity	4 benzenediol + O(2) = 4 benzosemiquinone + 2 H(2)O.
Cofactor	Binds 4 copper ions per monomer By similarity.
Subcellular location	Secreted › extracellular space › apoplastPotential.
Sequence similarities	Belongs to the multicopper oxidase family. Contains 3 plastocyanin-like domains.
Sequence caution	The sequence BAF29536.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Lignin degradation
Cellular component	Apoplast Secreted
Domain	Repeat Signal
Ligand	Copper Metal-binding
Molecular function	Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	lignin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	apoplast Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	copper ion binding Inferred from electronic annotation. Source: InterPro laccase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 579	556	Laccase-24		PRO_0000291911
Regions
	Domain	31 – 148	118	Plastocyanin-like 1
	Domain	159 – 322	164	Plastocyanin-like 2
	Domain	425 – 563	139	Plastocyanin-like 3
Sites
	Metal binding	82	1	Copper 1 By similarity
	Metal binding	84	1	Copper 2 By similarity
	Metal binding	127	1	Copper 2 By similarity
	Metal binding	129	1	Copper 3 By similarity
	Metal binding	480	1	Copper 4 By similarity
	Metal binding	483	1	Copper 1 By similarity
	Metal binding	485	1	Copper 3 By similarity
	Metal binding	542	1	Copper 3 By similarity
	Metal binding	543	1	Copper 4 By similarity
	Metal binding	544	1	Copper 2 By similarity
	Metal binding	548	1	Copper 4 By similarity
Amino acid modifications
	Glycosylation	34	1	N-linked (GlcNAc...) Potential
	Glycosylation	78	1	N-linked (GlcNAc...) Potential
	Glycosylation	110	1	N-linked (GlcNAc...) Potential
	Glycosylation	116	1	N-linked (GlcNAc...) Potential
	Glycosylation	204	1	N-linked (GlcNAc...) Potential
	Glycosylation	209	1	N-linked (GlcNAc...) Potential
	Glycosylation	219	1	N-linked (GlcNAc...) Potential
	Glycosylation	241	1	N-linked (GlcNAc...) Potential
	Glycosylation	312	1	N-linked (GlcNAc...) Potential
	Glycosylation	337	1	N-linked (GlcNAc...) Potential
	Glycosylation	348	1	N-linked (GlcNAc...) Potential
	Glycosylation	398	1	N-linked (GlcNAc...) Potential
	Glycosylation	405	1	N-linked (GlcNAc...) Potential
	Glycosylation	444	1	N-linked (GlcNAc...) Potential
	Glycosylation	462	1	N-linked (GlcNAc...) Potential
	Glycosylation	500	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q2QUN2-1 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: FD10AEC0CC9EB013	FASTA	579	63,459
	10 20 30 40 50 60 MARSWSLLLL PFALALVASV AQAAVVEYTF NVGNLSISQL CQQEMIITAV NGQLPGPTIV 70 80 90 100 110 120 ATEGDTVVVH MVNESPYNMT IHWHGIFQRG TPWADGPAMV TQCPVRPGGN YTYRFNVTGQ 130 140 150 160 170 180 EGTLWWHSHF SFLRATVYGA LIIKPRGGAK AYPFPVPDEE VVVILGEWWK TNVYDLQQRS 190 200 210 220 230 240 LVTGNPAPHA DAYTINGKPG DFYNCSAPNQ THKFELKQNK TYMLRIINAA LNTPLFFKVA 250 260 270 280 290 300 NHSFNVVAAD ACYTKPYKTD VVVISPGQTV DALLVPDAGV AAAVGGRYYM AVIPYNSAVN 310 320 330 340 350 360 AADPSFLYSL TNSTAIVEYG GGPATSPPMV PDMPEYNDTA TAHRFLSNMT ALVPNRVPLA 370 380 390 400 410 420 VDTHMFVTVS MGDTFCGPEQ TMCMPDDKGT IFASSMNNAS FILPNTTSML EAMYKGSIDG 430 440 450 460 470 480 VYTRDFPDTP PIVFDYTADA SDDNATLKHT FKSTKVKTLK YNSTVQMVLQ NTRLVSKESH 490 500 510 520 530 540 PMHLHGFNFF VLAQGFGNYN ETTDPAKFNL VDPQERNTVA VPTGGWAVIR FVADNPGVWF 550 560 570 MHCHFDAHLE FGLGMVFEVQ NGPTQETSLP PPPSDLPQC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The sequence of rice chromosomes 11 and 12, rich in disease resistance genes and recent gene duplications." The rice chromosomes 11 and 12 sequencing consortia BMC Biol. 3:20-20(2005) [PubMed: 16188032] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed: 15685292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	DP000011 Genomic DNA. Translation: ABA97304.1. AP008218 Genomic DNA. Translation: BAF29536.1. Sequence problems. CM000142 Genomic DNA. Translation: EAZ33360.1.
RefSeq	NP_001066517.1.
UniGene	Os.78609
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4351915.
KEGG	osa:4351915.
Organism-specific databases
Gramene	Q2QUN2.
Family and domain databases
InterPro	IPR001117. Cu-oxidase. IPR011706. Cu-oxidase_2. IPR011707. Cu-oxidase_3. IPR002355. Cu_oxidase_Cu_BS. IPR008972. Cupredoxin. IPR017761. Laccase. [Graphical view]
Gene3D	G3DSA:2.60.40.420. Cupredoxin. 3 hits.
Pfam	PF00394. Cu-oxidase. 1 hit. PF07731. Cu-oxidase_2. 1 hit. PF07732. Cu-oxidase_3. 1 hit. [Graphical view]
PROSITE	PS00079. MULTICOPPER_OXIDASE1. False negative. PS00080. MULTICOPPER_OXIDASE2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LAC24_ORYSJ

Accession

Primary (citable) accession number: Q2QUN2
Secondary accession number(s): Q0IP29

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
Last sequence update:	January 24, 2006
Last modified:	November 25, 2008
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)