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Protein

Phosphoglucan, water dikinase, chloroplastic

Gene

GWD3

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Mediates the incorporation of phosphate into starch-like phospho-alpha-glucan, mostly at the C-3 position of glucose units. May be required for starch degradation, suggesting that the phosphate content of starch regulates its degradability (By similarity).By similarity

Catalytic activityi

ATP + (phospho-alpha-glucan) + H2O = AMP + O-phospho-(phospho-alpha-glucan) + phosphate.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei776Tele-phosphohistidine intermediateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Protein family/group databases

CAZyiCBM20. Carbohydrate-Binding Module Family 20.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoglucan, water dikinase, chloroplastic (EC:2.7.9.5)
Gene namesi
Name:GWD3
Synonyms:PWD
Ordered Locus Names:Os12g0297500, LOC_Os12g20150
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
Proteomesi
  • UP000059680 Componenti: Chromosome 12

Subcellular locationi

  • Plastidchloroplast

  • Note: Starch granules during starch mobilization in darkness.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 56ChloroplastSequence analysisAdd BLAST56
ChainiPRO_000024025157 – 1206Phosphoglucan, water dikinase, chloroplasticAdd BLAST1150

Proteomic databases

PaxDbiQ2QTC2.

Expressioni

Gene expression databases

ExpressionAtlasiQ2QTC2. baseline and differential.
GenevisibleiQ2QTC2. OS.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi39947.LOC_Os12g20150.1.

Structurei

3D structure databases

ProteinModelPortaliQ2QTC2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini67 – 168CBM20PROSITE-ProRule annotationAdd BLAST102

Domaini

The N-terminal domain contains the alpha-glucan binding site, the central domain the pyrophosphate/phosphate carrier histidine, and the C-terminal domain the ATP binding site.By similarity

Sequence similaritiesi

Belongs to the PEP-utilizing enzyme family.Curated
Contains 1 CBM20 (carbohydrate binding type-20) domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IIIJ. Eukaryota.
COG0574. LUCA.
HOGENOMiHOG000265182.
InParanoidiQ2QTC2.
KOiK15535.
OMAiGKDKIWE.
OrthoDBiEOG093600ON.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
InterProiIPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR013784. Carb-bd-like_fold.
IPR002044. CBM_fam20.
IPR013783. Ig-like_fold.
IPR002192. PPDK_PEP-bd.
[Graphical view]
PfamiPF00686. CBM_20. 1 hit.
PF01326. PPDK_N. 1 hit.
[Graphical view]
SMARTiSM01065. CBM_2. 1 hit.
[Graphical view]
SUPFAMiSSF49452. SSF49452. 1 hit.
PROSITEiPS51166. CBM20. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q2QTC2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSLRPLETS LSIGGRPRRG LVLPPPGVGA GVLLRRGAMA LPGRRGFACR
60 70 80 90 100
GRSAASAAER TKEKKRRDSS KQPLVHLQVC LEHQVKFGEH VGIIGSTKEL
110 120 130 140 150
GSWEEQVELE WTTNGWVCQL KLPGETLVEF KFVIFLVGGK DKIWEDGNNR
160 170 180 190 200
VVELPKDGKF DIVCHWNRTE EPLELLGTPK FELVGEAEKN TGEDASASVT
210 220 230 240 250
FAPEKVQDIS VVENGDPAPE AESSKFGGQW QGSKTVFMRS NEHLNKEADR
260 270 280 290 300
MWDTTGLDGI ALKLVEGDKA SRNWWRKLEV VRGILSESFD DQSRLGALVY
310 320 330 340 350
SAIYLKWIYT GQISCFEDGG HHRPNKHAEI SRQIFRELEM MYYGKTTSAK
360 370 380 390 400
DVLVIRKIHP FLPSFKSEFT ASVPLTRIRD IAHRNDIPHD LKQEIKHTIQ
410 420 430 440 450
NKLHRNAGPE DLIATEVMLA RITKTPGEYS ETFVEQFTIF YSELKDFFNA
460 470 480 490 500
GSLFEQLESI KESLNESGLE VLSSFVETKR SLDQVDHAED LDKNDTIQIL
510 520 530 540 550
MTTLQSLSSL RSVLMKGLES GLRNDAPDNA IAMRQKWRLC EISLEDYSFV
560 570 580 590 600
LLSRFINTLE ALGGSASLAK DVARNTTLWD TTLDALVIGI NQVSFSGWKT
610 620 630 640 650
DECIAIGNEI LSWKQKGLSE SEGCEDGKYI WSLRLKATLD RARRLTEEYS
660 670 680 690 700
EALLSIFPEK VMVIGKALGI PDNSVRTYTE AEIRAGIVFQ VSKLCTVLQK
710 720 730 740 750
AIREVLGSTG WDVLVPGVAH GTLMRVERIL PGSLPSSVKE PVVLIVDKAD
760 770 780 790 800
GDEEVKAAGD NIVGVILLQE LPHLSHLGVR ARQENVVFVT CEYDDTVTDV
810 820 830 840 850
YLLEGKYIRL EASSINVNLS IVSEKNDNAV STEPNSTGNP FQQKLQNEFS
860 870 880 890 900
LPSDIEMPLQ MSKQKSKSGV NGSFAALELS EASVESAGAK AAACRTLSVL
910 920 930 940 950
ASLSNKVYSD QGVPAAFRVP SGAVIPFGSM EDALKKSGSL ESFTSLLEKI
960 970 980 990 1000
ETAKVENGEV DSLALELQAI ISHLSPPEET IIFLKRIFPQ DVRLIVRSSA
1010 1020 1030 1040 1050
NVEDLAGMSA AGLYDSIPNV SLMDPCAFGA AVGKVWASLY TRRAILSRRA
1060 1070 1080 1090 1100
AGVYQRDATM AVLVQEILQP DLSFVLHTVC PADHDPKVVQ AEVAPGLGET
1110 1120 1130 1140 1150
LASGTRGTPW RLSCNKFDGK VATLAFSNFS EEMVVHNSGP ANGEVIRLTV
1160 1170 1180 1190 1200
DYSKKPLSVD TTFRKQFGQR LAAIGQYLEQ KFGSAQDVEG CLVGKDIFIV

QSRPQP
Length:1,206
Mass (Da):132,895
Last modified:June 13, 2006 - v2
Checksum:i92A1E4D4CFBD54B5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DP000011 Genomic DNA. Translation: ABA97816.2.
AP008218 Genomic DNA. Translation: BAF29632.1.
AP014968 Genomic DNA. Translation: BAT16806.1.
RefSeqiXP_015620009.1. XM_015764523.1.
UniGeneiOs.15116.

Genome annotation databases

EnsemblPlantsiOS12T0297500-01; OS12T0297500-01; OS12G0297500.
GeneIDi4352028.
GrameneiOS12T0297500-01; OS12T0297500-01; OS12G0297500.
KEGGiosa:4352028.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DP000011 Genomic DNA. Translation: ABA97816.2.
AP008218 Genomic DNA. Translation: BAF29632.1.
AP014968 Genomic DNA. Translation: BAT16806.1.
RefSeqiXP_015620009.1. XM_015764523.1.
UniGeneiOs.15116.

3D structure databases

ProteinModelPortaliQ2QTC2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39947.LOC_Os12g20150.1.

Protein family/group databases

CAZyiCBM20. Carbohydrate-Binding Module Family 20.

Proteomic databases

PaxDbiQ2QTC2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS12T0297500-01; OS12T0297500-01; OS12G0297500.
GeneIDi4352028.
GrameneiOS12T0297500-01; OS12T0297500-01; OS12G0297500.
KEGGiosa:4352028.

Phylogenomic databases

eggNOGiENOG410IIIJ. Eukaryota.
COG0574. LUCA.
HOGENOMiHOG000265182.
InParanoidiQ2QTC2.
KOiK15535.
OMAiGKDKIWE.
OrthoDBiEOG093600ON.

Gene expression databases

ExpressionAtlasiQ2QTC2. baseline and differential.
GenevisibleiQ2QTC2. OS.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
InterProiIPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR013784. Carb-bd-like_fold.
IPR002044. CBM_fam20.
IPR013783. Ig-like_fold.
IPR002192. PPDK_PEP-bd.
[Graphical view]
PfamiPF00686. CBM_20. 1 hit.
PF01326. PPDK_N. 1 hit.
[Graphical view]
SMARTiSM01065. CBM_2. 1 hit.
[Graphical view]
SUPFAMiSSF49452. SSF49452. 1 hit.
PROSITEiPS51166. CBM20. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPWD_ORYSJ
AccessioniPrimary (citable) accession number: Q2QTC2
Secondary accession number(s): Q0INT3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: June 13, 2006
Last modified: October 5, 2016
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The reaction takes place in three steps, mediated by a phosphocarrier histidine residue located on the surface of the central domain. The two first partial reactions are catalyzed at an active site located on the C-terminal domain, and the third partial reaction is catalyzed at an active site located on the N-terminal domain. For catalytic turnover, the central domain swivels from the concave surface of the C-terminal domain to that of the N-terminal domain (By similarity).By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.