Q2QSB9 (AMPL1_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative leucine aminopeptidase 1
EC=3.4.11.1

Alternative name(s):
Leucyl aminopeptidase 1
Short name=LAP 1
Proline aminopeptidase 1
EC=3.4.11.5
Prolyl aminopeptidase 1

Gene names

Ordered Locus Names:

Os12g0434400, LOC_Os12g24650

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	542 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity.
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. Release of N-terminal proline from a peptide.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Subunit structure	Homohexamer Probable.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Zinc
Molecular function	Aminopeptidase Hydrolase Protease
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 542	542	Putative leucine aminopeptidase 1		PRO_0000247502
Sites
	Active site	323	1	Potential
	Active site	400	1	Potential
	Metal binding	294	1	Zinc 2 By similarity
	Metal binding	299	1	Zinc 1 By similarity
	Metal binding	299	1	Zinc 2 By similarity
	Metal binding	336	1	Zinc 2 By similarity
	Metal binding	396	1	Zinc 1 By similarity
	Metal binding	398	1	Zinc 1 By similarity
	Metal binding	398	1	Zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2QSB9 [UniParc]. Last modified July 11, 2006. Version 2. Checksum: BD6D2ED97D7CEB69	FASTA	542	57,142
	10 20 30 40 50 60 MPNVVDPPQI SFAAKDMDLT EWEGDILAVL VTETDVSKAT SSSSRFTNAA AALAKLDGEL 70 80 90 100 110 120 GGLLSEASAE EEFAGRAGQS VALRLPTAPG LHGFKRVCLV GVGNNMPSSA AACRSTGETI 130 140 150 160 170 180 AAVAKSAQAR SAAVALASPP PGWVQGEDLR LNAAAAVASG VVLGLHEDRR YKSDSKKVHL 190 200 210 220 230 240 KQVDLIGFGS GQEMGRKLQY ANHVSSAVIF AKELVNSPAN VLTPAVLAEE ASNIASSYSD 250 260 270 280 290 300 VLTATILDEE KCRELKMGSY LAVAAASANP PHFIHLCYKP PGGNVKRKLA IVGKGLTFDR 310 320 330 340 350 360 FYLSLDNLLI VTKFVCIGGY NIKIGAVTTI ELMKKDMGGS AALFGAAKAL GQIKPPGVEV 370 380 390 400 410 420 HFISAACENM ISGTGMRPGD IVTASNGKTI EVDNTDAEGR LTLADALVYA CKLGVDKIID 430 440 450 460 470 480 LATLTGYCRI ALGPSIAGIL TPSDELDKEV AAAYEASGEK FWRLPLEESY WEQMKSSVAD 490 500 510 520 530 540 MLNTGSPLGG AITAGLFLKQ FVDEKVKWMH VDMAGPVWNY KKQEATGFGV STLVEWVLIN SS

References

[1]	"The sequence of rice chromosomes 11 and 12, rich in disease resistance genes and recent gene duplications." The rice chromosomes 11 and 12 sequencing consortia BMC Biol. 3:20-20(2005) [PubMed: 16188032] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DP000011 Genomic DNA. Translation: ABA97701.2.
RefSeq	NP_001066684.1. NM_001073216.1.
UniGene	Os.79677.
3D structure databases
ProteinModelPortal	Q2QSB9.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2QSB9.
Protein family/group databases
MEROPS	M17.002.
Proteomic databases
PRIDE	Q2QSB9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os12g24650.1; LOC_Os12g24650.1; LOC_Os12g24650.
GeneID	4352123.
KEGG	osa:4352123.
Phylogenomic databases
GeneTree	EPGT00070000029661.
OMA	FISAACE.
PhylomeDB	Q2QSB9.
ProtClustDB	CLSN2683023.
Family and domain databases
InterPro	IPR000819. Peptidase_M17_C. IPR023042. Peptidase_M17_cytosol_amino. IPR008283. Peptidase_M17_N. [Graphical view]
KO	K01255.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPL1_ORYSJ

Accession

Primary (citable) accession number: Q2QSB9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	July 11, 2006
Last modified:	November 16, 2011
This is version 40 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program