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Q2QRX6

- DRTS_ORYSJ

UniProt

Q2QRX6 - DRTS_ORYSJ

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Protein

Putative bifunctional dihydrofolate reductase-thymidylate synthase

Gene

Os12g0446900

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Bifunctional enzyme. Involved in de novo dTMP biosynthesis. Key enzyme in folate metabolism. Can play two different roles depending on the source of dihydrofolate: de novo synthesis of tetrahydrofolate or recycling of the dihydrofolate released as one of the end products of the TS catalyzed reaction. Catalyzes an essential reaction for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP (By similarity).By similarity

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.
5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei16 – 161SubstrateBy similarity
Binding sitei103 – 1031Substrate; via carbonyl oxygenBy similarity
Binding sitei124 – 1241SubstrateBy similarity
Binding sitei231 – 2311dUMPBy similarity
Active sitei376 – 3761By similarity
Binding sitei377 – 3771dUMPBy similarity
Binding sitei407 – 4071dUMPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi2 – 87NADPBy similarity
Nucleotide bindingi40 – 423NADPBy similarity
Nucleotide bindingi61 – 644NADPBy similarity
Nucleotide bindingi104 – 1118NADPBy similarity
Nucleotide bindingi395 – 3995dUMPBy similarity
Nucleotide bindingi437 – 4393dUMPBy similarity

GO - Molecular functioni

  1. dihydrofolate reductase activity Source: UniProtKB-EC
  2. thymidylate synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. dTMP biosynthetic process Source: InterPro
  2. glycine biosynthetic process Source: InterPro
  3. one-carbon metabolic process Source: UniProtKB-KW
  4. tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Oxidoreductase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis, One-carbon metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative bifunctional dihydrofolate reductase-thymidylate synthase
Short name:
DHFR-TS
Including the following 2 domains:
Dihydrofolate reductase (EC:1.5.1.3)
Thymidylate synthase (EC:2.1.1.45)
Gene namesi
Ordered Locus Names:Os12g0446900, LOC_Os12g26060
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 12

Organism-specific databases

GrameneiQ2QRX6.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 494494Putative bifunctional dihydrofolate reductase-thymidylate synthasePRO_0000247485Add
BLAST

Expressioni

Gene expression databases

ExpressionAtlasiQ2QRX6. baseline.

Structurei

3D structure databases

ProteinModelPortaliQ2QRX6.
SMRiQ2QRX6. Positions 209-494.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 167167DHFRAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni170 – 494325Thymidylate synthaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the dihydrofolate reductase family.Curated
In the C-terminal section; belongs to the thymidylate synthase family.Curated

Phylogenomic databases

eggNOGiCOG0262.
InParanoidiQ2QRX6.

Family and domain databases

Gene3Di3.30.572.10. 1 hit.
3.40.430.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact.
InterProiIPR024072. DHFR-like_dom.
IPR012262. DHFR-TS.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
IPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00186. DHFR_1. 1 hit.
PF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF000389. DHFR-TS. 1 hit.
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF53597. SSF53597. 1 hit.
SSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 1 hit.
PROSITEiPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2QRX6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGIGKDGTLP WKLPGDLKFF KDITVTTSDP SKKNAVVMGR KTWESIPLKF
60 70 80 90 100
RPLPGRLNVI LTRSGSFDFA TAENVVICGS LDSALQLLAT TPYCLTVEKT
110 120 130 140 150
FIIGGGEILR QSLNAPACEA IHLTDIESSI ECDTFIPPID LSMFHPWYSS
160 170 180 190 200
FPVVENGIKH SFISFVRVTK SIAEANDSSG KELTGNDSKK VKFEIENFSF
210 220 230 240 250
LPKMIFERHE EYQYLNLVQD IIRNGAKKND RTGTGTVSKF GCQMRFNLRR
260 270 280 290 300
NFPLLTTKRV FWRGVLEELL WFISGSTNAK VLQEKGIHIW DGNASRQYLD
310 320 330 340 350
SIGLTQREEG DLGPVYGFQW RHFGAEYTDM HADYVGKGFD QLMDVIDKIK
360 370 380 390 400
NNPDDRRIIL SAWNPTDLKK MALPPCHMFA QFYVENGELS CQMYQRSADM
410 420 430 440 450
GLGVPFNIAS YSLLTCMIAQ VCDLSPGDFV HVIGDAHVYR THVEALEEQM
460 470 480 490
RKQPKPFPIL KINPVKKDID SFVTSDFKLV RYDPHHKIEM KMAV
Length:494
Mass (Da):55,977
Last modified:July 11, 2006 - v2
Checksum:i1F7F2D5162109A1B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DP000011 Genomic DNA. Translation: ABA98037.2.
AP008218 Genomic DNA. No translation available.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DP000011 Genomic DNA. Translation: ABA98037.2 .
AP008218 Genomic DNA. No translation available.

3D structure databases

ProteinModelPortali Q2QRX6.
SMRi Q2QRX6. Positions 209-494.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

Gramenei Q2QRX6.

Phylogenomic databases

eggNOGi COG0262.
InParanoidi Q2QRX6.

Enzyme and pathway databases

UniPathwayi UPA00077 ; UER00158 .

Gene expression databases

ExpressionAtlasi Q2QRX6. baseline.

Family and domain databases

Gene3Di 3.30.572.10. 1 hit.
3.40.430.10. 1 hit.
HAMAPi MF_00008. Thymidy_synth_bact.
InterProi IPR024072. DHFR-like_dom.
IPR012262. DHFR-TS.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
IPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view ]
Pfami PF00186. DHFR_1. 1 hit.
PF00303. Thymidylat_synt. 1 hit.
[Graphical view ]
PIRSFi PIRSF000389. DHFR-TS. 1 hit.
PRINTSi PR00108. THYMDSNTHASE.
SUPFAMi SSF53597. SSF53597. 1 hit.
SSF55831. SSF55831. 1 hit.
TIGRFAMsi TIGR03284. thym_sym. 1 hit.
PROSITEi PS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The sequence of rice chromosomes 11 and 12, rich in disease resistance genes and recent gene duplications."
    The rice chromosomes 11 and 12 sequencing consortia
    BMC Biol. 3:20-20(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.

Entry informationi

Entry nameiDRTS_ORYSJ
AccessioniPrimary (citable) accession number: Q2QRX6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: July 11, 2006
Last modified: October 29, 2014
This is version 57 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3