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Protein

Putative bifunctional dihydrofolate reductase-thymidylate synthase

Gene

Os12g0446900

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Bifunctional enzyme. Involved in de novo dTMP biosynthesis. Key enzyme in folate metabolism. Can play two different roles depending on the source of dihydrofolate: de novo synthesis of tetrahydrofolate or recycling of the dihydrofolate released as one of the end products of the TS catalyzed reaction. Catalyzes an essential reaction for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP (By similarity).By similarity

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.
5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate.
Proteins known to be involved in this subpathway in this organism are:
  1. Bifunctional dihydrofolate reductase-thymidylate synthase, Bifunctional dihydrofolate reductase-thymidylate synthase (Os11g0484400), Bifunctional dihydrofolate reductase-thymidylate synthase (OsJ_33908), Bifunctional dihydrofolate reductase-thymidylate synthase (Os12g0446900), Putative bifunctional dihydrofolate reductase-thymidylate synthase (Os12g0446900)
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei16SubstrateBy similarity1
Binding sitei103Substrate; via carbonyl oxygenBy similarity1
Binding sitei124SubstrateBy similarity1
Binding sitei231dUMPBy similarity1
Active sitei376By similarity1
Binding sitei377dUMPBy similarity1
Binding sitei407dUMPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi2 – 8NADPBy similarity7
Nucleotide bindingi40 – 42NADPBy similarity3
Nucleotide bindingi61 – 64NADPBy similarity4
Nucleotide bindingi104 – 111NADPBy similarity8
Nucleotide bindingi395 – 399dUMPBy similarity5
Nucleotide bindingi437 – 439dUMPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Multifunctional enzyme, Oxidoreductase, Transferase
Biological processNucleotide biosynthesis, One-carbon metabolism
LigandNADP

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158

Names & Taxonomyi

Protein namesi
Recommended name:
Putative bifunctional dihydrofolate reductase-thymidylate synthase
Short name:
DHFR-TS
Including the following 2 domains:
Dihydrofolate reductase (EC:1.5.1.3)
Thymidylate synthase (EC:2.1.1.45)
Gene namesi
Ordered Locus Names:Os12g0446900, LOC_Os12g26060
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000059680 Componenti: Chromosome 12

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002474851 – 494Putative bifunctional dihydrofolate reductase-thymidylate synthaseAdd BLAST494

Proteomic databases

PRIDEiQ2QRX6

Expressioni

Gene expression databases

ExpressionAtlasiQ2QRX6 differential
GenevisibleiQ2QRX6 OS

Structurei

3D structure databases

ProteinModelPortaliQ2QRX6
SMRiQ2QRX6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 167DHFRAdd BLAST167

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni170 – 494Thymidylate synthaseAdd BLAST325

Sequence similaritiesi

In the N-terminal section; belongs to the dihydrofolate reductase family.Curated
In the C-terminal section; belongs to the thymidylate synthase family.Curated

Phylogenomic databases

InParanoidiQ2QRX6

Family and domain databases

CDDicd00209 DHFR, 1 hit
cd00351 TS_Pyrimidine_HMase, 1 hit
Gene3Di3.30.572.10, 1 hit
3.40.430.10, 1 hit
HAMAPiMF_00008 Thymidy_synth_bact, 1 hit
InterProiView protein in InterPro
IPR024072 DHFR-like_dom_sf
IPR012262 DHFR-TS
IPR017925 DHFR_CS
IPR001796 DHFR_dom
IPR023451 Thymidate_synth/dCMP_Mease
IPR036926 Thymidate_synth/dCMP_Mease_sf
IPR000398 Thymidylate_synthase
IPR020940 Thymidylate_synthase_AS
PANTHERiPTHR11548:SF7 PTHR11548:SF7, 1 hit
PfamiView protein in Pfam
PF00186 DHFR_1, 1 hit
PF00303 Thymidylat_synt, 1 hit
PIRSFiPIRSF000389 DHFR-TS, 1 hit
PRINTSiPR00108 THYMDSNTHASE
SUPFAMiSSF53597 SSF53597, 1 hit
SSF55831 SSF55831, 1 hit
TIGRFAMsiTIGR03284 thym_sym, 1 hit
PROSITEiView protein in PROSITE
PS00075 DHFR_1, 1 hit
PS51330 DHFR_2, 1 hit
PS00091 THYMIDYLATE_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q2QRX6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGIGKDGTLP WKLPGDLKFF KDITVTTSDP SKKNAVVMGR KTWESIPLKF
60 70 80 90 100
RPLPGRLNVI LTRSGSFDFA TAENVVICGS LDSALQLLAT TPYCLTVEKT
110 120 130 140 150
FIIGGGEILR QSLNAPACEA IHLTDIESSI ECDTFIPPID LSMFHPWYSS
160 170 180 190 200
FPVVENGIKH SFISFVRVTK SIAEANDSSG KELTGNDSKK VKFEIENFSF
210 220 230 240 250
LPKMIFERHE EYQYLNLVQD IIRNGAKKND RTGTGTVSKF GCQMRFNLRR
260 270 280 290 300
NFPLLTTKRV FWRGVLEELL WFISGSTNAK VLQEKGIHIW DGNASRQYLD
310 320 330 340 350
SIGLTQREEG DLGPVYGFQW RHFGAEYTDM HADYVGKGFD QLMDVIDKIK
360 370 380 390 400
NNPDDRRIIL SAWNPTDLKK MALPPCHMFA QFYVENGELS CQMYQRSADM
410 420 430 440 450
GLGVPFNIAS YSLLTCMIAQ VCDLSPGDFV HVIGDAHVYR THVEALEEQM
460 470 480 490
RKQPKPFPIL KINPVKKDID SFVTSDFKLV RYDPHHKIEM KMAV
Length:494
Mass (Da):55,977
Last modified:July 11, 2006 - v2
Checksum:i1F7F2D5162109A1B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DP000011 Genomic DNA Translation: ABA98037.2
AP008218 Genomic DNA No translation available.
AP014968 Genomic DNA No translation available.

Genome annotation databases

EnsemblPlantsiOs12t0446900-01; Os12t0446900-01; Os12g0446900
GrameneiOs12t0446900-01; Os12t0446900-01; Os12g0446900

Similar proteinsi

Entry informationi

Entry nameiDRTS_ORYSJ
AccessioniPrimary (citable) accession number: Q2QRX6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: July 11, 2006
Last modified: May 23, 2018
This is version 81 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

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