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Q2QRX6 (DRTS_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative bifunctional dihydrofolate reductase-thymidylate synthase

Short name=DHFR-TS

Including the following 2 domains:

  1. Dihydrofolate reductase
    EC=1.5.1.3
  2. Thymidylate synthase
    EC=2.1.1.45
Gene names
Ordered Locus Names:Os12g0446900, LOC_Os12g26060
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length494 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Bifunctional enzyme. Involved in de novo dTMP biosynthesis. Key enzyme in folate metabolism. Can play two different roles depending on the source of dihydrofolate: de novo synthesis of tetrahydrofolate or recycling of the dihydrofolate released as one of the end products of the TS catalyzed reaction. Catalyzes an essential reaction for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP By similarity. HAMAP-Rule MF_00008

Catalytic activity

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH. HAMAP-Rule MF_00008

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP-Rule MF_00008

Pathway

Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1. HAMAP-Rule MF_00008

Sequence similarities

In the N-terminal section; belongs to the dihydrofolate reductase family.

In the C-terminal section; belongs to the thymidylate synthase family.

Contains 1 DHFR (dihydrofolate reductase) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 494494Putative bifunctional dihydrofolate reductase-thymidylate synthase HAMAP-Rule MF_00008
PRO_0000247485

Regions

Domain1 – 167167DHFR
Nucleotide binding2 – 87NADP By similarity
Nucleotide binding40 – 423NADP By similarity
Nucleotide binding61 – 644NADP By similarity
Nucleotide binding104 – 1118NADP By similarity
Nucleotide binding395 – 3995dUMP By similarity
Nucleotide binding437 – 4393dUMP By similarity
Region170 – 494325Thymidylate synthase HAMAP-Rule MF_00008

Sites

Active site3761 By similarity
Binding site161Substrate By similarity
Binding site1031Substrate; via carbonyl oxygen By similarity
Binding site1241Substrate By similarity
Binding site2311dUMP By similarity
Binding site3771dUMP By similarity
Binding site4071dUMP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2QRX6 [UniParc].

Last modified July 11, 2006. Version 2.
Checksum: 1F7F2D5162109A1B

FASTA49455,977
        10         20         30         40         50         60 
MGIGKDGTLP WKLPGDLKFF KDITVTTSDP SKKNAVVMGR KTWESIPLKF RPLPGRLNVI 

        70         80         90        100        110        120 
LTRSGSFDFA TAENVVICGS LDSALQLLAT TPYCLTVEKT FIIGGGEILR QSLNAPACEA 

       130        140        150        160        170        180 
IHLTDIESSI ECDTFIPPID LSMFHPWYSS FPVVENGIKH SFISFVRVTK SIAEANDSSG 

       190        200        210        220        230        240 
KELTGNDSKK VKFEIENFSF LPKMIFERHE EYQYLNLVQD IIRNGAKKND RTGTGTVSKF 

       250        260        270        280        290        300 
GCQMRFNLRR NFPLLTTKRV FWRGVLEELL WFISGSTNAK VLQEKGIHIW DGNASRQYLD 

       310        320        330        340        350        360 
SIGLTQREEG DLGPVYGFQW RHFGAEYTDM HADYVGKGFD QLMDVIDKIK NNPDDRRIIL 

       370        380        390        400        410        420 
SAWNPTDLKK MALPPCHMFA QFYVENGELS CQMYQRSADM GLGVPFNIAS YSLLTCMIAQ 

       430        440        450        460        470        480 
VCDLSPGDFV HVIGDAHVYR THVEALEEQM RKQPKPFPIL KINPVKKDID SFVTSDFKLV 

       490 
RYDPHHKIEM KMAV 

« Hide

References

[1]"The sequence of rice chromosomes 11 and 12, rich in disease resistance genes and recent gene duplications."
The rice chromosomes 11 and 12 sequencing consortia
BMC Biol. 3:20-20(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DP000011 Genomic DNA. Translation: ABA98037.2.

3D structure databases

ProteinModelPortalQ2QRX6.
SMRQ2QRX6. Positions 209-494.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

GrameneQ2QRX6.

Phylogenomic databases

eggNOGCOG0262.

Enzyme and pathway databases

UniPathwayUPA00077; UER00158.

Family and domain databases

Gene3D3.30.572.10. 1 hit.
3.40.430.10. 1 hit.
HAMAPMF_00008. Thymidy_synth_bact.
InterProIPR024072. DHFR-like_dom.
IPR012262. DHFR-TS.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
IPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamPF00186. DHFR_1. 1 hit.
PF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PIRSFPIRSF000389. DHFR-TS. 1 hit.
PRINTSPR00108. THYMDSNTHASE.
SUPFAMSSF53597. SSF53597. 1 hit.
SSF55831. SSF55831. 1 hit.
TIGRFAMsTIGR03284. thym_sym. 1 hit.
PROSITEPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDRTS_ORYSJ
AccessionPrimary (citable) accession number: Q2QRX6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: July 11, 2006
Last modified: February 19, 2014
This is version 54 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

PATHWAY comments

Index of metabolic and biosynthesis pathways