Q2QNF7 (DAPF_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Diaminopimelate epimerase, chloroplastic
Short name=DAP epimerase
EC=5.1.1.7

Gene names

Name:	DAPF
Ordered Locus Names:	Os12g0567200, LOC_Os12g37960
ORF Names:	OsJ_035115

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; DL-2,6-diaminopimelate from LL-2,6-diaminopimelate: step 1/1.
Subcellular location	Plastid › chloroplast Potential.
Sequence similarities	Belongs to the diaminopimelate epimerase family.
Sequence caution	The sequence BAF30079.2 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Lysine biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	lysine biosynthetic process via diaminopimelate Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	diaminopimelate epimerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 44	44	Chloroplast Potential
	Chain	45 – 354	310	Diaminopimelate epimerase, chloroplastic		PRO_0000307181
Regions
	Compositional bias	55 – 59	5	Poly-Ala
Sites
	Active site	142	1	By similarity
	Active site	297	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2QNF7 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: D8511696965BD8AD	FASTA	354	37,830
	10 20 30 40 50 60 MSSATAAATA TIAAAAAKLA ATPAPAPSRR RLTLRGNPTA RRCVAAMAVS TPRSAAAAAF 70 80 90 100 110 120 LERRESERAL HFVKYQGLGN DFIMVDNRDS AVPKVTPEEA AKLCDRNFGV GADGVIFVMP 130 140 150 160 170 180 GVNGADYTMR IFNSDGSEPE MCGNGVRCFA RFIAELENLQ GTHSFKIHTG AGLIIPEIQN 190 200 210 220 230 240 DGKVKVDMGQ PILSGPDIPT KLPSTKNEAV VQADLAVDGS TWQVTCVSMG NPHCVTFGTK 250 260 270 280 290 300 ELKVLHVDDL KLSDIGPKFE HHEMFPARTN TEFVEVLSRS HLKMRVWERG AGATLACGTG 310 320 330 340 350 ACAVVVAAVL EGRAERKCVV DLPGGPLEIE WREDDNHIYM TGPAEAVFYG SAVH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The sequence of rice chromosomes 11 and 12, rich in disease resistance genes and recent gene duplications." The rice chromosomes 11 and 12 sequencing consortia BMC Biol. 3:20-20(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[5]	"Oryza sativa full-length cDNA." The rice full-length cDNA consortium Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 340-354. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DP000011 Genomic DNA. Translation: ABA99594.1. AP008218 Genomic DNA. Translation: BAF30079.2. Sequence problems. CM000149 Genomic DNA. Translation: EAZ20906.1. AK242455 mRNA. No translation available.
RefSeq	NP_001067060.2. NM_001073592.2.
UniGene	Os.21433.
3D structure databases
HSSP	HSSP built from PDB template 2GKE based on UniProtKB P44859.
ProteinModelPortal	Q2QNF7.
ModBase	Search...
Proteomic databases
PRIDE	Q2QNF7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os12g37960.1; LOC_Os12g37960.1; LOC_Os12g37960. LOC_Os12g37960.2; LOC_Os12g37960.2; LOC_Os12g37960.
GeneID	4352558.
KEGG	osa:4352558.
Organism-specific databases
Gramene	Q2QNF7.
Phylogenomic databases
eggNOG	COG0253.
KO	K01778.
OMA	VPHAVIF.
ProtClustDB	PLN02536.
Enzyme and pathway databases
UniPathway	UPA00034; UER00025.
Gene expression databases
ArrayExpress	Q2QNF7.
Family and domain databases
InterPro	IPR018510. DAP_epimerase_AS. IPR001653. DAP_epimerase_DapF. [Graphical view]
Pfam	PF01678. DAP_epimerase. 2 hits. [Graphical view]
TIGRFAMs	TIGR00652. DapF. 1 hit.
PROSITE	PS01326. DAP_EPIMERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPF_ORYSJ

Accession

Primary (citable) accession number: Q2QNF7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 23, 2007
Last sequence update:	January 24, 2006
Last modified:	April 3, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program