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Protein

Diaminopimelate epimerase, chloroplastic

Gene

DAPF

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.

Pathwayi: L-lysine biosynthesis via DAP pathway

This protein is involved in step 1 of the subpathway that synthesizes DL-2,6-diaminopimelate from LL-2,6-diaminopimelate.
Proteins known to be involved in this subpathway in this organism are:
  1. Diaminopimelate epimerase, chloroplastic (DAPF)
This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes DL-2,6-diaminopimelate from LL-2,6-diaminopimelate, the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei142By similarity1
Active sitei297By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Amino-acid biosynthesis, Lysine biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00034; UER00025.

Names & Taxonomyi

Protein namesi
Recommended name:
Diaminopimelate epimerase, chloroplastic (EC:5.1.1.7)
Short name:
DAP epimerase
Gene namesi
Name:DAPF
Ordered Locus Names:Os12g0567200, LOC_Os12g37960
ORF Names:OsJ_035115
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
Proteomesi
  • UP000059680 Componenti: Chromosome 12

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 44ChloroplastSequence analysisAdd BLAST44
ChainiPRO_000030718145 – 354Diaminopimelate epimerase, chloroplasticAdd BLAST310

Proteomic databases

PaxDbiQ2QNF7.

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os12g37960.1.

Structurei

3D structure databases

ProteinModelPortaliQ2QNF7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi55 – 59Poly-Ala5

Sequence similaritiesi

Belongs to the diaminopimelate epimerase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IHJB. Eukaryota.
COG0253. LUCA.
InParanoidiQ2QNF7.
KOiK01778.

Family and domain databases

HAMAPiMF_00197. DAP_epimerase. 1 hit.
InterProiIPR018510. DAP_epimerase_AS.
IPR001653. DAP_epimerase_DapF.
[Graphical view]
PANTHERiPTHR31689. PTHR31689. 1 hit.
PfamiPF01678. DAP_epimerase. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00652. DapF. 1 hit.
PROSITEiPS01326. DAP_EPIMERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q2QNF7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSATAAATA TIAAAAAKLA ATPAPAPSRR RLTLRGNPTA RRCVAAMAVS
60 70 80 90 100
TPRSAAAAAF LERRESERAL HFVKYQGLGN DFIMVDNRDS AVPKVTPEEA
110 120 130 140 150
AKLCDRNFGV GADGVIFVMP GVNGADYTMR IFNSDGSEPE MCGNGVRCFA
160 170 180 190 200
RFIAELENLQ GTHSFKIHTG AGLIIPEIQN DGKVKVDMGQ PILSGPDIPT
210 220 230 240 250
KLPSTKNEAV VQADLAVDGS TWQVTCVSMG NPHCVTFGTK ELKVLHVDDL
260 270 280 290 300
KLSDIGPKFE HHEMFPARTN TEFVEVLSRS HLKMRVWERG AGATLACGTG
310 320 330 340 350
ACAVVVAAVL EGRAERKCVV DLPGGPLEIE WREDDNHIYM TGPAEAVFYG

SAVH
Length:354
Mass (Da):37,830
Last modified:January 24, 2006 - v1
Checksum:iD8511696965BD8AD
GO

Sequence cautioni

The sequence BAF30079 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DP000011 Genomic DNA. Translation: ABA99594.1.
AP008218 Genomic DNA. Translation: BAF30079.2. Sequence problems.
AP014968 Genomic DNA. No translation available.
CM000149 Genomic DNA. Translation: EAZ20906.1.
AK242455 mRNA. No translation available.
RefSeqiXP_015620224.1. XM_015764738.1.
XP_015620225.1. XM_015764739.1.
UniGeneiOs.21433.

Genome annotation databases

GeneIDi4352558.
KEGGiosa:4352558.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DP000011 Genomic DNA. Translation: ABA99594.1.
AP008218 Genomic DNA. Translation: BAF30079.2. Sequence problems.
AP014968 Genomic DNA. No translation available.
CM000149 Genomic DNA. Translation: EAZ20906.1.
AK242455 mRNA. No translation available.
RefSeqiXP_015620224.1. XM_015764738.1.
XP_015620225.1. XM_015764739.1.
UniGeneiOs.21433.

3D structure databases

ProteinModelPortaliQ2QNF7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39947.LOC_Os12g37960.1.

Proteomic databases

PaxDbiQ2QNF7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi4352558.
KEGGiosa:4352558.

Phylogenomic databases

eggNOGiENOG410IHJB. Eukaryota.
COG0253. LUCA.
InParanoidiQ2QNF7.
KOiK01778.

Enzyme and pathway databases

UniPathwayiUPA00034; UER00025.

Family and domain databases

HAMAPiMF_00197. DAP_epimerase. 1 hit.
InterProiIPR018510. DAP_epimerase_AS.
IPR001653. DAP_epimerase_DapF.
[Graphical view]
PANTHERiPTHR31689. PTHR31689. 1 hit.
PfamiPF01678. DAP_epimerase. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00652. DapF. 1 hit.
PROSITEiPS01326. DAP_EPIMERASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDAPF_ORYSJ
AccessioniPrimary (citable) accession number: Q2QNF7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: January 24, 2006
Last modified: November 2, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.