Reviewed,
UniProtKB/Swiss-Prot Q2QMG2 (MCCA_ORYSJ)
Last modified
January 19, 2010.
Version 40.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
Customize display | text xml rdf/xml gff fasta |
Names and origin
| Protein names | Recommended name: Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial Short name=MCCase subunit alpha EC=6.4.1.4 Alternative name(s): 3-methylcrotonyl-CoA carboxylase 1 3-methylcrotonyl-CoA:carbon dioxide ligase subunit alpha | ||||
| Gene names |
| ||||
| Organism | Oryza sativa subsp. japonica (Rice) | ||||
| Taxonomic identifier | 39947 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza |
Protein attributes
| Sequence length | 737 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Catalytic activity | ATP + 3-methylcrotonoyl-CoA + HCO3- = ADP + phosphate + 3-methylglutaconyl-CoA. |
| Cofactor | Biotin. Binds 2 manganese ions per subunit By similarity. |
| Pathway | |
| Subunit structure | Probably a heterodimer composed of biotin-containing alpha subunits and beta subunits By similarity. |
| Subcellular location | Mitochondrion matrix Probable. |
| Induction | Induced during senescence. Ref.3 |
| Sequence similarities | Contains 1 ATP-grasp domain. Contains 1 biotin carboxylation domain. Contains 1 biotinyl-binding domain. |
| Sequence caution | The sequence ABA99832.2 differs from that shown. Reason: Erroneous gene model prediction. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Mitochondrion |
| Domain | Transit peptide |
| Ligand | ATP-binding Biotin Manganese Metal-binding Nucleotide-binding |
| Molecular function | Ligase |
| Gene Ontology (GO) | |
| Biological process | metabolic process Inferred from electronic annotation. Source: InterPro |
| Cellular component | mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW biotin bindingInferred from electronic annotation. Source: InterPro manganese ion bindingInferred from electronic annotation. Source: UniProtKB-KW methylcrotonoyl-CoA carboxylase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – 33 | 33 | Mitochondrion Potential | ||||||
| Chain | 34 – 737 | 704 | Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial | PRO_0000247483 | |||||
Regions | |||||||||
| Domain | 38 – 485 | 448 | Biotin carboxylation | ||||||
| Domain | 157 – 355 | 199 | ATP-grasp | ||||||
| Domain | 664 – 731 | 68 | Biotinyl-binding | ||||||
| Nucleotide binding | 185 – 246 | 62 | ATP By similarity | ||||||
| Compositional bias | 624 – 635 | 12 | His-rich | ||||||
Sites | |||||||||
| Active site | 330 | 1 | By similarity | ||||||
| Metal binding | 312 | 1 | Manganese 1 By similarity | ||||||
| Metal binding | 326 | 1 | Manganese 1 By similarity | ||||||
| Metal binding | 326 | 1 | Manganese 2 By similarity | ||||||
| Metal binding | 328 | 1 | Manganese 2 By similarity | ||||||
| Binding site | 153 | 1 | ATP By similarity | ||||||
| Binding site | 237 | 1 | ATP By similarity | ||||||
| Binding site | 272 | 1 | ATP By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 698 | 1 | N6-biotinyllysine By similarity | ||||||
Sequences
| ||||||||||||||||||
References
| « Hide 'large scale' references | |
| [1] | "The sequence of rice chromosomes 11 and 12, rich in disease resistance genes and recent gene duplications." The rice chromosomes 11 and 12 sequencing consortia BMC Biol. 3:20-20(2005) [PubMed: 16188032] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare. |
| [2] | "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare. |
| [3] | "Leaf senescence in rice plants: cloning and characterization of senescence up-regulated genes." Lee R.-H., Wang C.-H., Huang L.-T., Chen S.-C.G. J. Exp. Bot. 52:1117-1121(2001) [PubMed: 11432928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 535-737, INDUCTION. Strain: cv. Tainung 67. Tissue: Leaf. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | DP000011 Genomic DNA. Translation: ABA99831.2. DP000011 Genomic DNA. Translation: ABA99832.2. Sequence problems. DP000011 Genomic DNA. Translation: ABA99833.1. AK121511 mRNA. No translation available. AF251074 mRNA. Translation: AAL65397.1. |
| RefSeq | NP_001067226.1. |
| UniGene | Os.19628 |
3D structure databases | |
| SMR | Q2QMG2. Positions 39-488, 664-731. |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 4352741. |
| KEGG | osa:4352741. |
Organism-specific databases | |
| Gramene | Q8W2G0. |
Family and domain databases | |
| InterPro | IPR011761. ATP-grasp. IPR013816. ATP_grasp_subdomain_2. IPR001882. Biotin_BS. IPR011764. Biotin_carboxylation_dom. IPR005482. Biotin_COase_C. IPR000089. Biotin_lipoyl. IPR005479. CarbamoylP_synth_lsu_ATP-bd. IPR005481. CarbamoylP_synth_lsu_N. IPR013817. Pre-ATP_grasp. IPR016185. PreATP-grasp-like. IPR011054. Rudment_hybrid_motif. IPR011053. Single_hybrid_motif. [Graphical view] |
| Gene3D | G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit. |
| Pfam | PF02785. Biotin_carb_C. 1 hit. PF00364. Biotin_lipoyl. 1 hit. PF00289. CPSase_L_chain. 1 hit. PF02786. CPSase_L_D2. 1 hit. [Graphical view] |
| SMART | SM00878. Biotin_carb_C. 1 hit. [Graphical view] |
| PROSITE | PS50975. ATP_GRASP. 1 hit. PS50979. BC. 1 hit. PS00188. BIOTIN. 1 hit. PS50968. BIOTINYL_LIPOYL. 1 hit. PS00866. CPSASE_1. 1 hit. PS00867. CPSASE_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | MCCA_ORYSJ | ||||||||
| Accession | Primary (citable) accession number: Q2QMG2 Secondary accession number(s): Q2QMG3, Q2QMG4, Q8W2G0 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||
Relevant documents
| Oryza sativa (rice) Index of Oryza sativa entries and their corresponding gene designations |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


