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Q2QKL5 (PCS3_LOTJA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 31. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione gamma-glutamylcysteinyltransferase 3

EC=2.3.2.15
Alternative name(s):
LjPCS3-7N
Phytochelatin synthase 3
Gene names
Name:PCS3
OrganismLotus japonicus (Lotus corniculatus var. japonicus)
Taxonomic identifier34305 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeLoteaeLotus

Protein attributes

Sequence length479 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in the synthesis of phytochelatins (PC) and homophytochelatins (hPC), the heavy-metal-binding peptides of plants. Ref.2

Catalytic activity

Glutathione + (Glu(-Cys))(n)-Gly = Gly + (Glu(-Cys))(n+1)-Gly.

Enzyme regulation

Requires cadmium for activity By similarity.

Tissue specificity

Expressed in roots, nodules and leaves. Ref.2

Induction

By cadmium. Ref.2

Sequence similarities

Belongs to the phytochelatin synthase family.

Contains 1 peptidase C83 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 479479Glutathione gamma-glutamylcysteinyltransferase 3
PRO_0000287215

Regions

Domain1 – 221221Peptidase C83
Compositional bias352 – 37019Cys-rich

Sites

Active site561 By similarity
Active site1621 By similarity
Active site1801 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2QKL5 [UniParc].

Last modified January 24, 2006. Version 1.
Checksum: D9D1E442D74E27C2

FASTA47953,187
        10         20         30         40         50         60 
MASAGLYRRV LPSPPAIDFA SPEGKKIFVE ALGQGTMEGF FKLVSYYQTQ SEPAYCGLAT 

        70         80         90        100        110        120 
LTVVLNALSI DPGRKWKGPW RWFDDSMLDC CEPLEKIKVQ GITFGKVACL ARCNGAHVEA 

       130        140        150        160        170        180 
FRSNESTVSD FRDRVISCCS SEDRHLIVSY HRSGLKQTGE GHFSPIGGYH AERDMVLILD 

       190        200        210        220        230        240 
VTRYKYPPHW VPLTLLWDAM NTIDRATGLQ RGYMIISKLK RAPSILYTVS CRHEGWSSVA 

       250        260        270        280        290        300 
KFLTENVPLL LKSEDLKDIQ EVLSVVFKSP PSELREFITW IAEVRRQEDG NLTLSEEEKG 

       310        320        330        340        350        360 
RLAIKADILE QIRTTTLFKH VTSWLDSQRS RCRTIAKLQD RDMLPELAAG VCCQGACLLT 

       370        380        390        400        410        420 
GCCLPGGKCC SQIDVKHLNV DHKNIVTLVS GTVASGSSSE QGVDVLVPLC QMGPEGHCIG 

       430        440        450        460        470 
MHPSTADVLT VLLLALPLHT WSGIKEEKLC AEVTSLLTTE NLPPLLQEEV RFSLETVLF 

« Hide

References

[1]"Lotus japonicus phytochelatin synthase mRNA (pcs2)."
Naya L., Ramos J., Becana M.
Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Root.
[2]"Phytochelatin synthases of the model legume Lotus japonicus. A small multigene family with differential response to cadmium and alternatively spliced variants."
Ramos J., Clemente M.R., Naya L., Loscos J., Perez-Rontome C., Sato S., Tabata S., Becana M.
Plant Physiol. 143:1110-1118(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION.
Strain: cv. Miyakojima MG-20.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DQ013041 mRNA. Translation: AAY81941.1.
DQ013040 Genomic DNA. Translation: AAY81940.1.

3D structure databases

ProteinModelPortalQ2QKL5.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSC83.002.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR007719. Phytochelatin_synthase.
IPR015407. Phytochelatin_synthase_C.
[Graphical view]
PfamPF05023. Phytochelatin. 1 hit.
PF09328. Phytochelatin_C. 1 hit.
[Graphical view]
PROSITEPS51443. PCS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePCS3_LOTJA
AccessionPrimary (citable) accession number: Q2QKL5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: January 24, 2006
Last modified: February 19, 2014
This is version 31 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries