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Protein

Glutathione gamma-glutamylcysteinyltransferase 3

Gene

PCS3

Organism
Lotus japonicus (Lotus corniculatus var. japonicus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the synthesis of phytochelatins (PC) and homophytochelatins (hPC), the heavy-metal-binding peptides of plants.1 Publication

Catalytic activityi

Glutathione + (Glu(-Cys))(n)-Gly = Gly + (Glu(-Cys))(n+1)-Gly.PROSITE-ProRule annotation

Enzyme regulationi

Requires cadmium for activity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei56 – 561PROSITE-ProRule annotation
Active sitei162 – 1621PROSITE-ProRule annotation
Active sitei180 – 1801PROSITE-ProRule annotation

GO - Molecular functioni

  1. glutathione gamma-glutamylcysteinyltransferase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. phytochelatin biosynthetic process Source: InterPro
  2. response to metal ion Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Ligandi

Cadmium, Metal-binding

Enzyme and pathway databases

BRENDAi2.3.2.15. 3076.

Protein family/group databases

MEROPSiC83.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione gamma-glutamylcysteinyltransferase 3 (EC:2.3.2.15)
Alternative name(s):
LjPCS3-7N
Phytochelatin synthase 3
Gene namesi
Name:PCS3
OrganismiLotus japonicus (Lotus corniculatus var. japonicus)
Taxonomic identifieri34305 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeLoteaeLotus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 479479Glutathione gamma-glutamylcysteinyltransferase 3PRO_0000287215Add
BLAST

Expressioni

Tissue specificityi

Expressed in roots, nodules and leaves.1 Publication

Inductioni

By cadmium.1 Publication

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 221221Peptidase C83PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi352 – 37019Cys-richAdd
BLAST

Sequence similaritiesi

Belongs to the phytochelatin synthase family.PROSITE-ProRule annotation
Contains 1 peptidase C83 domain.PROSITE-ProRule annotation

Family and domain databases

InterProiIPR007719. Phytochelatin_synthase.
IPR015407. Phytochelatin_synthase_C.
[Graphical view]
PfamiPF05023. Phytochelatin. 1 hit.
PF09328. Phytochelatin_C. 1 hit.
[Graphical view]
PROSITEiPS51443. PCS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2QKL5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASAGLYRRV LPSPPAIDFA SPEGKKIFVE ALGQGTMEGF FKLVSYYQTQ
60 70 80 90 100
SEPAYCGLAT LTVVLNALSI DPGRKWKGPW RWFDDSMLDC CEPLEKIKVQ
110 120 130 140 150
GITFGKVACL ARCNGAHVEA FRSNESTVSD FRDRVISCCS SEDRHLIVSY
160 170 180 190 200
HRSGLKQTGE GHFSPIGGYH AERDMVLILD VTRYKYPPHW VPLTLLWDAM
210 220 230 240 250
NTIDRATGLQ RGYMIISKLK RAPSILYTVS CRHEGWSSVA KFLTENVPLL
260 270 280 290 300
LKSEDLKDIQ EVLSVVFKSP PSELREFITW IAEVRRQEDG NLTLSEEEKG
310 320 330 340 350
RLAIKADILE QIRTTTLFKH VTSWLDSQRS RCRTIAKLQD RDMLPELAAG
360 370 380 390 400
VCCQGACLLT GCCLPGGKCC SQIDVKHLNV DHKNIVTLVS GTVASGSSSE
410 420 430 440 450
QGVDVLVPLC QMGPEGHCIG MHPSTADVLT VLLLALPLHT WSGIKEEKLC
460 470
AEVTSLLTTE NLPPLLQEEV RFSLETVLF
Length:479
Mass (Da):53,187
Last modified:January 23, 2006 - v1
Checksum:iD9D1E442D74E27C2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ013041 mRNA. Translation: AAY81941.1.
DQ013040 Genomic DNA. Translation: AAY81940.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ013041 mRNA. Translation: AAY81941.1.
DQ013040 Genomic DNA. Translation: AAY81940.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiC83.002.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi2.3.2.15. 3076.

Family and domain databases

InterProiIPR007719. Phytochelatin_synthase.
IPR015407. Phytochelatin_synthase_C.
[Graphical view]
PfamiPF05023. Phytochelatin. 1 hit.
PF09328. Phytochelatin_C. 1 hit.
[Graphical view]
PROSITEiPS51443. PCS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Lotus japonicus phytochelatin synthase mRNA (pcs2)."
    Naya L., Ramos J., Becana M.
    Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Root.
  2. "Phytochelatin synthases of the model legume Lotus japonicus. A small multigene family with differential response to cadmium and alternatively spliced variants."
    Ramos J., Clemente M.R., Naya L., Loscos J., Perez-Rontome C., Sato S., Tabata S., Becana M.
    Plant Physiol. 143:1110-1118(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION.
    Strain: cv. Miyakojima MG-20.

Entry informationi

Entry nameiPCS3_LOTJA
AccessioniPrimary (citable) accession number: Q2QKL5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 14, 2007
Last sequence update: January 23, 2006
Last modified: March 31, 2015
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.