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Q2QKL5

- PCS3_LOTJA

UniProt

Q2QKL5 - PCS3_LOTJA

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Protein
Glutathione gamma-glutamylcysteinyltransferase 3
Gene
PCS3
Organism
Lotus japonicus (Lotus corniculatus var. japonicus)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Involved in the synthesis of phytochelatins (PC) and homophytochelatins (hPC), the heavy-metal-binding peptides of plants.1 Publication

Catalytic activityi

Glutathione + (Glu(-Cys))(n)-Gly = Gly + (Glu(-Cys))(n+1)-Gly.

Enzyme regulationi

Requires cadmium for activity By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei56 – 561 By similarity
Active sitei162 – 1621 By similarity
Active sitei180 – 1801 By similarity

GO - Molecular functioni

  1. glutathione gamma-glutamylcysteinyltransferase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. phytochelatin biosynthetic process Source: InterPro
  2. response to metal ion Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Ligandi

Cadmium, Metal-binding

Protein family/group databases

MEROPSiC83.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione gamma-glutamylcysteinyltransferase 3 (EC:2.3.2.15)
Alternative name(s):
LjPCS3-7N
Phytochelatin synthase 3
Gene namesi
Name:PCS3
OrganismiLotus japonicus (Lotus corniculatus var. japonicus)
Taxonomic identifieri34305 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeLoteaeLotus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 479479Glutathione gamma-glutamylcysteinyltransferase 3
PRO_0000287215Add
BLAST

Expressioni

Tissue specificityi

Expressed in roots, nodules and leaves.1 Publication

Inductioni

By cadmium.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ2QKL5.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 221221Peptidase C83
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi352 – 37019Cys-rich
Add
BLAST

Sequence similaritiesi

Family and domain databases

InterProiIPR007719. Phytochelatin_synthase.
IPR015407. Phytochelatin_synthase_C.
[Graphical view]
PfamiPF05023. Phytochelatin. 1 hit.
PF09328. Phytochelatin_C. 1 hit.
[Graphical view]
PROSITEiPS51443. PCS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2QKL5-1 [UniParc]FASTAAdd to Basket

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MASAGLYRRV LPSPPAIDFA SPEGKKIFVE ALGQGTMEGF FKLVSYYQTQ    50
SEPAYCGLAT LTVVLNALSI DPGRKWKGPW RWFDDSMLDC CEPLEKIKVQ 100
GITFGKVACL ARCNGAHVEA FRSNESTVSD FRDRVISCCS SEDRHLIVSY 150
HRSGLKQTGE GHFSPIGGYH AERDMVLILD VTRYKYPPHW VPLTLLWDAM 200
NTIDRATGLQ RGYMIISKLK RAPSILYTVS CRHEGWSSVA KFLTENVPLL 250
LKSEDLKDIQ EVLSVVFKSP PSELREFITW IAEVRRQEDG NLTLSEEEKG 300
RLAIKADILE QIRTTTLFKH VTSWLDSQRS RCRTIAKLQD RDMLPELAAG 350
VCCQGACLLT GCCLPGGKCC SQIDVKHLNV DHKNIVTLVS GTVASGSSSE 400
QGVDVLVPLC QMGPEGHCIG MHPSTADVLT VLLLALPLHT WSGIKEEKLC 450
AEVTSLLTTE NLPPLLQEEV RFSLETVLF 479
Length:479
Mass (Da):53,187
Last modified:January 24, 2006 - v1
Checksum:iD9D1E442D74E27C2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ013041 mRNA. Translation: AAY81941.1.
DQ013040 Genomic DNA. Translation: AAY81940.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ013041 mRNA. Translation: AAY81941.1 .
DQ013040 Genomic DNA. Translation: AAY81940.1 .

3D structure databases

ProteinModelPortali Q2QKL5.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C83.002.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR007719. Phytochelatin_synthase.
IPR015407. Phytochelatin_synthase_C.
[Graphical view ]
Pfami PF05023. Phytochelatin. 1 hit.
PF09328. Phytochelatin_C. 1 hit.
[Graphical view ]
PROSITEi PS51443. PCS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Lotus japonicus phytochelatin synthase mRNA (pcs2)."
    Naya L., Ramos J., Becana M.
    Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Root.
  2. "Phytochelatin synthases of the model legume Lotus japonicus. A small multigene family with differential response to cadmium and alternatively spliced variants."
    Ramos J., Clemente M.R., Naya L., Loscos J., Perez-Rontome C., Sato S., Tabata S., Becana M.
    Plant Physiol. 143:1110-1118(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION.
    Strain: cv. Miyakojima MG-20.

Entry informationi

Entry nameiPCS3_LOTJA
AccessioniPrimary (citable) accession number: Q2QKL5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: January 24, 2006
Last modified: February 19, 2014
This is version 31 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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