Q2QEI3 (IOD1_XENLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type I iodothyronine deiodinase
EC=1.97.1.10

Alternative name(s):
5DI
DIOI
Type 1 DI
Type-I 5'-deiodinase

Gene names

Name:

dio1

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	252 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into T3 (3,5,3'-triiodothyronine) and of T3 into T2 (3,3'-diiodothyronine) By similarity.
Catalytic activity	3,5,3'-triiodo-L-thyronine + iodide + A + H+ = L-thyroxine + AH2.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein By similarity.
Sequence similarities	Belongs to the iodothyronine deiodinase family.

Ontologies

Keywords
Biological process	Thyroid hormones biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Coding sequence diversity	Selenocysteine
Domain	Transmembrane Transmembrane helix
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological_process	hormone biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	thyroxine 5'-deiodinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 252	252	Type I iodothyronine deiodinase		PRO_0000318637
Regions
	Transmembrane	18 – 38	21	Helical; Potential
Sites
	Active site	130	1
Amino acid modifications
	Non-standard residue	130	1	Selenocysteine

Sequences

	Sequence		Length	Mass (Da)
	Q2QEI3 [UniParc]. Last modified February 26, 2008. Version 2. Checksum: 026288D0E636D131	FASTA	252	29,187
	10 20 30 40 50 60 MESLLQTIKL MLRYIQKALI LFFLFLYVVV GKVLMFLFPQ TMASVLKSRF EISGVHDPKF 70 80 90 100 110 120 QYEDWGPTFF TYKFLRSVLE IMWMRLEDEA FVGHSAPNTP VVDLSGELHH IWDYLQGTRP 130 140 150 160 170 180 LVLSFGSCTU PPFLFRLGEF NKLVNEFNSI ADFLIIYIDE AHAADEWALK NNLHIKKHRS 190 200 210 220 230 240 LQDRLAAAKR LMEESPSCPV VLDTMSNLCS AKYAALPERL YILQEGKIIY KGKMGPWGYK 250 PEEVCSVLEK KK

References

[1]

"Characterization of recombinant Xenopus laevis type I iodothyronine deiodinase: substitution of a proline residue in the catalytic center by serine (Pro132Ser) restores sensitivity to 6-propyl-2-thiouracil." Kuiper G.G., Klootwijk W., Morvan Dubois G., Destree O., Darras V.M., Van der Geyten S., Demeneix B., Visser T.J. Endocrinology 147:3519-3529(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ098656 mRNA. Translation: AAZ43088.1.
RefSeq	NP_001089136.1. NM_001095667.1.
UniGene	Xl.17649.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	733447.
KEGG	xla:733447.
Organism-specific databases
CTD	1733.
Xenbase	XB-GENE-979948. dio1.
Phylogenomic databases
HOVERGEN	HBG000099.
KO	K01562.
Family and domain databases
Gene3D	3.40.30.10. 1 hit.
InterPro	IPR000643. Iodothyronine_deiodinase. IPR008261. Iodothyronine_deiodinase_AS. IPR012336. Thioredoxin-like_fold. [Graphical view]
PANTHER	PTHR11781. PTHR11781. 1 hit.
Pfam	PF00837. T4_deiodinase. 1 hit. [Graphical view]
PIRSF	PIRSF001330. IOD. 1 hit.
SUPFAM	SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS01205. T4_DEIODINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IOD1_XENLA

Accession

Primary (citable) accession number: Q2QEI3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	February 26, 2008
Last modified:	March 6, 2013
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program