Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q2QDF6

- DET2_GOSHI

UniProt

Q2QDF6 - DET2_GOSHI

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Steroid 5-alpha-reductase DET2

Gene

DET2

Organism
Gossypium hirsutum (Upland cotton) (Gossypium mexicanum)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Involved in a reduction step in the biosynthesis of the plant steroid, brassinolide. Promotes cotton fibers (seed trichomes) initiation and elongation.1 Publication

Catalytic activityi

A 3-oxo-5-alpha-steroid + NADP+ = a 3-oxo-Delta(4)-steroid + NADPH.1 Publication

Pathwayi

GO - Molecular functioni

  1. 3-oxo-5-alpha-steroid 4-dehydrogenase activity Source: InterPro
  2. cholestenone 5-alpha-reductase activity Source: UniProtKB-EC
  3. progesterone 5-alpha-reductase activity Source: UniProtKB

GO - Biological processi

  1. brassinosteroid biosynthetic process Source: UniProtKB-UniPathway
  2. oxidation-reduction process Source: UniProtKB
  3. seed trichome elongation Source: UniProtKB
  4. seed trichome initiation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00381.

Names & Taxonomyi

Protein namesi
Recommended name:
Steroid 5-alpha-reductase DET2 (EC:1.3.1.22)
Short name:
GhDET2
Gene namesi
Name:DET2
OrganismiGossypium hirsutum (Upland cotton) (Gossypium mexicanum)
Taxonomic identifieri3635 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsMalvalesMalvaceaeMalvoideaeGossypium

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: InterPro
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Impaired fiber initiation and elongation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 258258Steroid 5-alpha-reductase DET2PRO_0000418516Add
BLAST

Expressioni

Tissue specificityi

Accumulates in fibers (seed trichomes) during both their initiation and elongation phases. Also present in roots, hypocotyls, leaves, flowers and ovules, and barely in cotyledons.2 Publications

Structurei

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei8 – 2821HelicalSequence AnalysisAdd
BLAST
Transmembranei49 – 6921HelicalSequence AnalysisAdd
BLAST
Transmembranei77 – 9721HelicalSequence AnalysisAdd
BLAST
Transmembranei109 – 12921HelicalSequence AnalysisAdd
BLAST
Transmembranei144 – 16421HelicalSequence AnalysisAdd
BLAST
Transmembranei201 – 22121HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the steroid 5-alpha reductase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR016636. 3-oxo-5-alpha-steroid_4-DH.
IPR001104. 3-oxo-5_a-steroid_4-DH_C.
[Graphical view]
PfamiPF02544. Steroid_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF015596. 5_alpha-SR2. 1 hit.
PROSITEiPS50244. S5A_REDUCTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2QDF6 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASDQTLFHY CLLTLYIIAL PTWISLYFLQ APYGKHNRPG WGPTISPPLA
60 70 80 90 100
WFLMESPTLW LTFFLFPSGQ HFYNPKSFLL ISPFLFHYFN RTVLYPLRLA
110 120 130 140 150
RNTTQTRGFP VSVAFMAFGF NLLNGYLQAR WVSHYKDDYE NEELFWWRFL
160 170 180 190 200
AGLLIFVVGM WVNVRADKVL VGLKKQGDGG YKIPRGGLFE LVSCPNYFGE
210 220 230 240 250
IMEWFGWAVM TWSWVGFGFF LYTCANLMPR ARATRLWYLE KFKDDYPKDR

KAVIPFIY
Length:258
Mass (Da):30,421
Last modified:January 24, 2006 - v1
Checksum:i17489CA9E27964B2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ116446 mRNA. Translation: AAZ83346.1.
AY141136 mRNA. Translation: AAN28012.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ116446 mRNA. Translation: AAZ83346.1 .
AY141136 mRNA. Translation: AAN28012.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00381 .

Family and domain databases

InterProi IPR016636. 3-oxo-5-alpha-steroid_4-DH.
IPR001104. 3-oxo-5_a-steroid_4-DH_C.
[Graphical view ]
Pfami PF02544. Steroid_dh. 1 hit.
[Graphical view ]
PIRSFi PIRSF015596. 5_alpha-SR2. 1 hit.
PROSITEi PS50244. S5A_REDUCTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Transcriptome profiling, molecular biological, and physiological studies reveal a major role for ethylene in cotton fiber cell elongation."
    Shi Y.-H., Zhu S.-W., Mao X.-Z., Feng J.-X., Qin Y.-M., Zhang L., Cheng J., Wei L.-P., Wang Z.-Y., Zhu Y.-X.
    Plant Cell 18:651-664(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: cv. Xuzhou142.
  2. "GhDET2, a steroid 5alpha-reductase, plays an important role in cotton fiber cell initiation and elongation."
    Luo M., Xiao Y., Li X., Lu X., Deng W., Li D., Hou L., Hu M., Li Y., Pei Y.
    Plant J. 51:419-430(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, CATALYTIC ACTIVITY, TISSUE SPECIFICITY.
    Strain: cv. Xuzhou142.
    Tissue: Fiber.

Entry informationi

Entry nameiDET2_GOSHI
AccessioniPrimary (citable) accession number: Q2QDF6
Secondary accession number(s): Q8H1T2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: January 24, 2006
Last modified: October 1, 2014
This is version 29 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3