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Protein

Steroid 5-alpha-reductase DET2

Gene

DET2

Organism
Gossypium hirsutum (Upland cotton) (Gossypium mexicanum)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in a reduction step in the biosynthesis of the plant steroid, brassinolide. Promotes cotton fibers (seed trichomes) initiation and elongation.1 Publication

Catalytic activityi

A 3-oxo-5-alpha-steroid + NADP+ = a 3-oxo-Delta(4)-steroid + NADPH.1 Publication

Pathway: brassinosteroid biosynthesis

This protein is involved in the pathway brassinosteroid biosynthesis, which is part of Plant hormone biosynthesis.
View all proteins of this organism that are known to be involved in the pathway brassinosteroid biosynthesis and in Plant hormone biosynthesis.

GO - Molecular functioni

GO - Biological processi

  • brassinosteroid biosynthetic process Source: UniProtKB-UniPathway
  • oxidation-reduction process Source: UniProtKB
  • seed trichome elongation Source: UniProtKB
  • seed trichome initiation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00381.

Names & Taxonomyi

Protein namesi
Recommended name:
Steroid 5-alpha-reductase DET2 (EC:1.3.1.22)
Short name:
GhDET2
Gene namesi
Name:DET2
OrganismiGossypium hirsutum (Upland cotton) (Gossypium mexicanum)
Taxonomic identifieri3635 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsMalvalesMalvaceaeMalvoideaeGossypium

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei8 – 2821HelicalSequence AnalysisAdd
BLAST
Transmembranei49 – 6921HelicalSequence AnalysisAdd
BLAST
Transmembranei77 – 9721HelicalSequence AnalysisAdd
BLAST
Transmembranei109 – 12921HelicalSequence AnalysisAdd
BLAST
Transmembranei144 – 16421HelicalSequence AnalysisAdd
BLAST
Transmembranei201 – 22121HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Impaired fiber initiation and elongation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 258258Steroid 5-alpha-reductase DET2PRO_0000418516Add
BLAST

Expressioni

Tissue specificityi

Accumulates in fibers (seed trichomes) during both their initiation and elongation phases. Also present in roots, hypocotyls, leaves, flowers and ovules, and barely in cotyledons.2 Publications

Family & Domainsi

Sequence similaritiesi

Belongs to the steroid 5-alpha reductase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR016636. 3-oxo-5-alpha-steroid_4-DH.
IPR001104. 3-oxo-5_a-steroid_4-DH_C.
[Graphical view]
PfamiPF02544. Steroid_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF015596. 5_alpha-SR2. 1 hit.
PROSITEiPS50244. S5A_REDUCTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2QDF6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASDQTLFHY CLLTLYIIAL PTWISLYFLQ APYGKHNRPG WGPTISPPLA
60 70 80 90 100
WFLMESPTLW LTFFLFPSGQ HFYNPKSFLL ISPFLFHYFN RTVLYPLRLA
110 120 130 140 150
RNTTQTRGFP VSVAFMAFGF NLLNGYLQAR WVSHYKDDYE NEELFWWRFL
160 170 180 190 200
AGLLIFVVGM WVNVRADKVL VGLKKQGDGG YKIPRGGLFE LVSCPNYFGE
210 220 230 240 250
IMEWFGWAVM TWSWVGFGFF LYTCANLMPR ARATRLWYLE KFKDDYPKDR

KAVIPFIY
Length:258
Mass (Da):30,421
Last modified:January 24, 2006 - v1
Checksum:i17489CA9E27964B2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ116446 mRNA. Translation: AAZ83346.1.
AY141136 mRNA. Translation: AAN28012.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ116446 mRNA. Translation: AAZ83346.1.
AY141136 mRNA. Translation: AAN28012.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00381.

Family and domain databases

InterProiIPR016636. 3-oxo-5-alpha-steroid_4-DH.
IPR001104. 3-oxo-5_a-steroid_4-DH_C.
[Graphical view]
PfamiPF02544. Steroid_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF015596. 5_alpha-SR2. 1 hit.
PROSITEiPS50244. S5A_REDUCTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Transcriptome profiling, molecular biological, and physiological studies reveal a major role for ethylene in cotton fiber cell elongation."
    Shi Y.-H., Zhu S.-W., Mao X.-Z., Feng J.-X., Qin Y.-M., Zhang L., Cheng J., Wei L.-P., Wang Z.-Y., Zhu Y.-X.
    Plant Cell 18:651-664(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: cv. Xuzhou142.
  2. "GhDET2, a steroid 5alpha-reductase, plays an important role in cotton fiber cell initiation and elongation."
    Luo M., Xiao Y., Li X., Lu X., Deng W., Li D., Hou L., Hu M., Li Y., Pei Y.
    Plant J. 51:419-430(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, CATALYTIC ACTIVITY, TISSUE SPECIFICITY.
    Strain: cv. Xuzhou142.
    Tissue: Fiber.

Entry informationi

Entry nameiDET2_GOSHI
AccessioniPrimary (citable) accession number: Q2QDF6
Secondary accession number(s): Q8H1T2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: January 24, 2006
Last modified: October 1, 2014
This is version 29 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.