Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q2QDF6

- DET2_GOSHI

UniProt

Q2QDF6 - DET2_GOSHI

Protein

Steroid 5-alpha-reductase DET2

Gene

DET2

Organism
Gossypium hirsutum (Upland cotton) (Gossypium mexicanum)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 29 (01 Oct 2014)
      Sequence version 1 (24 Jan 2006)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Involved in a reduction step in the biosynthesis of the plant steroid, brassinolide. Promotes cotton fibers (seed trichomes) initiation and elongation.1 Publication

    Catalytic activityi

    A 3-oxo-5-alpha-steroid + NADP+ = a 3-oxo-Delta(4)-steroid + NADPH.1 Publication

    Pathwayi

    GO - Molecular functioni

    1. 3-oxo-5-alpha-steroid 4-dehydrogenase activity Source: InterPro
    2. cholestenone 5-alpha-reductase activity Source: UniProtKB-EC
    3. progesterone 5-alpha-reductase activity Source: UniProtKB

    GO - Biological processi

    1. brassinosteroid biosynthetic process Source: UniProtKB-UniPathway
    2. oxidation-reduction process Source: UniProtKB
    3. seed trichome elongation Source: UniProtKB
    4. seed trichome initiation Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    UniPathwayiUPA00381.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Steroid 5-alpha-reductase DET2 (EC:1.3.1.22)
    Short name:
    GhDET2
    Gene namesi
    Name:DET2
    OrganismiGossypium hirsutum (Upland cotton) (Gossypium mexicanum)
    Taxonomic identifieri3635 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsMalvalesMalvaceaeMalvoideaeGossypium

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: InterPro
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Impaired fiber initiation and elongation.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 258258Steroid 5-alpha-reductase DET2PRO_0000418516Add
    BLAST

    Expressioni

    Tissue specificityi

    Accumulates in fibers (seed trichomes) during both their initiation and elongation phases. Also present in roots, hypocotyls, leaves, flowers and ovules, and barely in cotyledons.2 Publications

    Structurei

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei8 – 2821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei49 – 6921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei77 – 9721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei109 – 12921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei144 – 16421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei201 – 22121HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the steroid 5-alpha reductase family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Family and domain databases

    InterProiIPR016636. 3-oxo-5-alpha-steroid_4-DH.
    IPR001104. 3-oxo-5_a-steroid_4-DH_C.
    [Graphical view]
    PfamiPF02544. Steroid_dh. 1 hit.
    [Graphical view]
    PIRSFiPIRSF015596. 5_alpha-SR2. 1 hit.
    PROSITEiPS50244. S5A_REDUCTASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q2QDF6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASDQTLFHY CLLTLYIIAL PTWISLYFLQ APYGKHNRPG WGPTISPPLA    50
    WFLMESPTLW LTFFLFPSGQ HFYNPKSFLL ISPFLFHYFN RTVLYPLRLA 100
    RNTTQTRGFP VSVAFMAFGF NLLNGYLQAR WVSHYKDDYE NEELFWWRFL 150
    AGLLIFVVGM WVNVRADKVL VGLKKQGDGG YKIPRGGLFE LVSCPNYFGE 200
    IMEWFGWAVM TWSWVGFGFF LYTCANLMPR ARATRLWYLE KFKDDYPKDR 250
    KAVIPFIY 258
    Length:258
    Mass (Da):30,421
    Last modified:January 24, 2006 - v1
    Checksum:i17489CA9E27964B2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ116446 mRNA. Translation: AAZ83346.1.
    AY141136 mRNA. Translation: AAN28012.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ116446 mRNA. Translation: AAZ83346.1 .
    AY141136 mRNA. Translation: AAN28012.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00381 .

    Family and domain databases

    InterProi IPR016636. 3-oxo-5-alpha-steroid_4-DH.
    IPR001104. 3-oxo-5_a-steroid_4-DH_C.
    [Graphical view ]
    Pfami PF02544. Steroid_dh. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF015596. 5_alpha-SR2. 1 hit.
    PROSITEi PS50244. S5A_REDUCTASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Transcriptome profiling, molecular biological, and physiological studies reveal a major role for ethylene in cotton fiber cell elongation."
      Shi Y.-H., Zhu S.-W., Mao X.-Z., Feng J.-X., Qin Y.-M., Zhang L., Cheng J., Wei L.-P., Wang Z.-Y., Zhu Y.-X.
      Plant Cell 18:651-664(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Strain: cv. Xuzhou142.
    2. "GhDET2, a steroid 5alpha-reductase, plays an important role in cotton fiber cell initiation and elongation."
      Luo M., Xiao Y., Li X., Lu X., Deng W., Li D., Hou L., Hu M., Li Y., Pei Y.
      Plant J. 51:419-430(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, CATALYTIC ACTIVITY, TISSUE SPECIFICITY.
      Strain: cv. Xuzhou142.
      Tissue: Fiber.

    Entry informationi

    Entry nameiDET2_GOSHI
    AccessioniPrimary (citable) accession number: Q2QDF6
    Secondary accession number(s): Q8H1T2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 5, 2012
    Last sequence update: January 24, 2006
    Last modified: October 1, 2014
    This is version 29 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3