Q2QDF6 (DET2_GOSHI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Steroid 5-alpha-reductase DET2
Short name=GhDET2
EC=1.3.1.22

Gene names

Name:

DET2

Organism

Gossypium hirsutum (Upland cotton) (Gossypium mexicanum)

Taxonomic identifier

3635 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Malvales › Malvaceae › Malvoideae › Gossypium

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in a reduction step in the biosynthesis of the plant steroid, brassinolide. Promotes cotton fibers (seed trichomes) initiation and elongation. Ref.2
Catalytic activity	A 3-oxo-5-alpha-steroid + NADP+ = a 3-oxo-Delta(4)-steroid + NADPH. Ref.2
Pathway	Plant hormone biosynthesis; brassinosteroid biosynthesis.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Tissue specificity	Accumulates in fibers (seed trichomes) during both their initiation and elongation phases. Also present in roots, hypocotyls, leaves, flowers and ovules, and barely in cotyledons. Ref.1 Ref.2
Disruption phenotype	Impaired fiber initiation and elongation. Ref.2
Sequence similarities	Belongs to the steroid 5-alpha reductase family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological_process	brassinosteroid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway seed trichome elongation Inferred from mutant phenotype Ref.2. Source: UniProtKB seed trichome initiation Inferred from mutant phenotype Ref.2. Source: UniProtKB
Cellular_component	cytoplasm Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	3-oxo-5-alpha-steroid 4-dehydrogenase activity Inferred from electronic annotation. Source: InterPro cholestenone 5-alpha-reductase activity Inferred from electronic annotation. Source: EC progesterone 5-alpha-reductase activity Inferred from direct assay Ref.2. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 258	258	Steroid 5-alpha-reductase DET2		PRO_0000418516
Regions
	Transmembrane	8 – 28	21	Helical; Potential
	Transmembrane	49 – 69	21	Helical; Potential
	Transmembrane	77 – 97	21	Helical; Potential
	Transmembrane	109 – 129	21	Helical; Potential
	Transmembrane	144 – 164	21	Helical; Potential
	Transmembrane	201 – 221	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q2QDF6 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: 17489CA9E27964B2	FASTA	258	30,421
	10 20 30 40 50 60 MASDQTLFHY CLLTLYIIAL PTWISLYFLQ APYGKHNRPG WGPTISPPLA WFLMESPTLW 70 80 90 100 110 120 LTFFLFPSGQ HFYNPKSFLL ISPFLFHYFN RTVLYPLRLA RNTTQTRGFP VSVAFMAFGF 130 140 150 160 170 180 NLLNGYLQAR WVSHYKDDYE NEELFWWRFL AGLLIFVVGM WVNVRADKVL VGLKKQGDGG 190 200 210 220 230 240 YKIPRGGLFE LVSCPNYFGE IMEWFGWAVM TWSWVGFGFF LYTCANLMPR ARATRLWYLE 250 KFKDDYPKDR KAVIPFIY

References

[1]	"Transcriptome profiling, molecular biological, and physiological studies reveal a major role for ethylene in cotton fiber cell elongation." Shi Y.-H., Zhu S.-W., Mao X.-Z., Feng J.-X., Qin Y.-M., Zhang L., Cheng J., Wei L.-P., Wang Z.-Y., Zhu Y.-X. Plant Cell 18:651-664(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Strain: cv. Xuzhou142.
[2]	"GhDET2, a steroid 5alpha-reductase, plays an important role in cotton fiber cell initiation and elongation." Luo M., Xiao Y., Li X., Lu X., Deng W., Li D., Hou L., Hu M., Li Y., Pei Y. Plant J. 51:419-430(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, CATALYTIC ACTIVITY, TISSUE SPECIFICITY. Strain: cv. Xuzhou142. Tissue: Fiber.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ116446 mRNA. Translation: AAZ83346.1. AY141136 mRNA. Translation: AAN28012.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00381.
Family and domain databases
InterPro	IPR016636. 3-oxo-5-alpha-steroid_4-DH. IPR001104. 3-oxo-5_a-steroid_4-DH_C. [Graphical view]
Pfam	PF02544. Steroid_dh. 1 hit. [Graphical view]
PIRSF	PIRSF015596. 5_alpha-SR2. 1 hit.
PROSITE	PS50244. S5A_REDUCTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DET2_GOSHI

Accession

Primary (citable) accession number: Q2QDF6
Secondary accession number(s): Q8H1T2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2012
Last sequence update:	January 24, 2006
Last modified:	February 6, 2013
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program