Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q2QDF6 (DET2_GOSHI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 28. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Steroid 5-alpha-reductase DET2

Short name=GhDET2
EC=1.3.1.22
Gene names
Name:DET2
OrganismGossypium hirsutum (Upland cotton) (Gossypium mexicanum)
Taxonomic identifier3635 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsMalvalesMalvaceaeMalvoideaeGossypium

Protein attributes

Sequence length258 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in a reduction step in the biosynthesis of the plant steroid, brassinolide. Promotes cotton fibers (seed trichomes) initiation and elongation. Ref.2

Catalytic activity

A 3-oxo-5-alpha-steroid + NADP+ = a 3-oxo-Delta(4)-steroid + NADPH. Ref.2

Pathway

Plant hormone biosynthesis; brassinosteroid biosynthesis.

Subcellular location

Membrane; Multi-pass membrane protein Potential.

Tissue specificity

Accumulates in fibers (seed trichomes) during both their initiation and elongation phases. Also present in roots, hypocotyls, leaves, flowers and ovules, and barely in cotyledons. Ref.1 Ref.2

Disruption phenotype

Impaired fiber initiation and elongation. Ref.2

Sequence similarities

Belongs to the steroid 5-alpha reductase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 258258Steroid 5-alpha-reductase DET2
PRO_0000418516

Regions

Transmembrane8 – 2821Helical; Potential
Transmembrane49 – 6921Helical; Potential
Transmembrane77 – 9721Helical; Potential
Transmembrane109 – 12921Helical; Potential
Transmembrane144 – 16421Helical; Potential
Transmembrane201 – 22121Helical; Potential

Sequences

Sequence LengthMass (Da)Tools
Q2QDF6 [UniParc].

Last modified January 24, 2006. Version 1.
Checksum: 17489CA9E27964B2

FASTA25830,421
        10         20         30         40         50         60 
MASDQTLFHY CLLTLYIIAL PTWISLYFLQ APYGKHNRPG WGPTISPPLA WFLMESPTLW 

        70         80         90        100        110        120 
LTFFLFPSGQ HFYNPKSFLL ISPFLFHYFN RTVLYPLRLA RNTTQTRGFP VSVAFMAFGF 

       130        140        150        160        170        180 
NLLNGYLQAR WVSHYKDDYE NEELFWWRFL AGLLIFVVGM WVNVRADKVL VGLKKQGDGG 

       190        200        210        220        230        240 
YKIPRGGLFE LVSCPNYFGE IMEWFGWAVM TWSWVGFGFF LYTCANLMPR ARATRLWYLE 

       250 
KFKDDYPKDR KAVIPFIY 

« Hide

References

[1]"Transcriptome profiling, molecular biological, and physiological studies reveal a major role for ethylene in cotton fiber cell elongation."
Shi Y.-H., Zhu S.-W., Mao X.-Z., Feng J.-X., Qin Y.-M., Zhang L., Cheng J., Wei L.-P., Wang Z.-Y., Zhu Y.-X.
Plant Cell 18:651-664(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Strain: cv. Xuzhou142.
[2]"GhDET2, a steroid 5alpha-reductase, plays an important role in cotton fiber cell initiation and elongation."
Luo M., Xiao Y., Li X., Lu X., Deng W., Li D., Hou L., Hu M., Li Y., Pei Y.
Plant J. 51:419-430(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, CATALYTIC ACTIVITY, TISSUE SPECIFICITY.
Strain: cv. Xuzhou142.
Tissue: Fiber.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DQ116446 mRNA. Translation: AAZ83346.1.
AY141136 mRNA. Translation: AAN28012.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00381.

Family and domain databases

InterProIPR016636. 3-oxo-5-alpha-steroid_4-DH.
IPR001104. 3-oxo-5_a-steroid_4-DH_C.
[Graphical view]
PfamPF02544. Steroid_dh. 1 hit.
[Graphical view]
PIRSFPIRSF015596. 5_alpha-SR2. 1 hit.
PROSITEPS50244. S5A_REDUCTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDET2_GOSHI
AccessionPrimary (citable) accession number: Q2QDF6
Secondary accession number(s): Q8H1T2
Entry history
Integrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: January 24, 2006
Last modified: April 16, 2014
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways