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Protein

AP-4 complex subunit mu-1

Gene

Ap4m1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the adaptor protein complex 4 (AP-4). Adaptor protein complexes are vesicle coat components involved both in vesicle formation and cargo selection. They control the vesicular transport of proteins in different trafficking pathways. AP-4 forms a non clathrin-associated coat on vesicles departing the trans-Golgi network (TGN) and may be involved in the targeting of proteins from the trans-Golgi network (TGN) to the endosomal-lysosomal system. It is also involved in protein sorting to the basolateral membrane in epithelial cells and the proper asymmetric localization of somatodendritic proteins in neurons. Within AP-4, the mu-type subunit AP4M1 is directly involved in the recognition and binding of tyrosine-based sorting signals found in the cytoplasmic part of cargos (By similarity). The adaptor protein complex 4 (AP-4) may also recognize other types of sorting signal (PubMed:14572453).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-432720. Lysosome Vesicle Biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
AP-4 complex subunit mu-1Curated
Alternative name(s):
AP-4 adaptor complex mu subunit
Adaptor-related protein complex 4 subunit mu-1
Mu subunit of AP-4
Mu-adaptin-related protein 2
Short name:
mu-ARP2
Mu4-adaptin
Short name:
mu4
Gene namesi
Name:Ap4m1Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi1310233. Ap4m1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endosome, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002297501 – 453AP-4 complex subunit mu-1CuratedAdd BLAST453

Proteomic databases

PaxDbiQ2PWT8.

Expressioni

Tissue specificityi

High levels in the olfactory bulb, the cerebral cortex, the granule and Purkinje cell layers of the cerebellar cortex and the CA3 region of the hippocampus. Low levels found in molecular layer of cerebellum.1 Publication

Gene expression databases

BgeeiENSRNOG00000001353.

Interactioni

Subunit structurei

Adaptor protein complex 4 (AP-4) is a heterotetramer composed of two large adaptins (epsilon-type subunit AP4E1 and beta-type subunit AP4B1), a medium adaptin (mu-type subunit AP4M1) and a small adaptin (sigma-type AP4S1). Interacts with tyrosine-based sorting signals on the cytoplasmic tail of cargo proteins such as APP, LAMP2 and NAGPA (By similarity). Interacts with the C-terminal domain of GRID2 (PubMed:14572453). Interacts with GRIA1 and GRIA2; the interaction is indirect via CACNG3. Interacts with CACNG3; CACNG3 associates GRIA1 and GRIA2 with the adaptor protein complex 4 (AP-4) to target them to the somatodendritic compartment of neurons (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000001827.

Structurei

3D structure databases

ProteinModelPortaliQ2PWT8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini184 – 452MHDPROSITE-ProRule annotationAdd BLAST269

Sequence similaritiesi

Belongs to the adaptor complexes medium subunit family.Sequence analysis

Phylogenomic databases

eggNOGiKOG0937. Eukaryota.
ENOG410XPFS. LUCA.
GeneTreeiENSGT00530000062779.
HOGENOMiHOG000173245.
HOVERGENiHBG102734.
InParanoidiQ2PWT8.
KOiK12402.
OMAiIHIRHSG.
OrthoDBiEOG091G0ISV.
PhylomeDBiQ2PWT8.
TreeFamiTF329745.

Family and domain databases

InterProiView protein in InterPro
IPR036168. AP2_Mu_C_sf.
IPR022775. AP_mu_sigma_su.
IPR001392. Clathrin_mu.
IPR018240. Clathrin_mu_CS.
IPR011012. Longin-like_dom_sf.
IPR028565. MHD.
PfamiView protein in Pfam
PF00928. Adap_comp_sub. 1 hit.
PF01217. Clat_adaptor_s. 1 hit.
PIRSFiPIRSF005992. Clathrin_mu. 1 hit.
PRINTSiPR00314. CLATHRINADPT.
SUPFAMiSSF49447. SSF49447. 1 hit.
SSF64356. SSF64356. 1 hit.
PROSITEiView protein in PROSITE
PS00991. CLAT_ADAPTOR_M_2. 1 hit.
PS51072. MHD. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2PWT8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MISQFFILSS KGDPLIYKDF RGDSGGRDVA ELFYRKLTGL PGGESPVVMY
60 70 80 90 100
HDDRHFIHIR HSGLYLVATT SENVSPFSLL ELLSRLATLL GDYCGSLNEG
110 120 130 140 150
TISRNVALVY ELLDEVLDYG YVQTTSTDML RNFIQTEAAV SKPFSLFDLS
160 170 180 190 200
SVGLFGAETQ QNRVAPSSAA SRPVLSSRSD QSQKNEVFLD VVERLSVLIA
210 220 230 240 250
SNGSLLKVDV QGEIRLKSFL PSSSEICIGL TEEFCVGKSE LRGYGPGIRV
260 270 280 290 300
DEVSFHSSVN LDEFESHRIL HLQPPQGELT VMRYQLSDDL PSPLPFRLFP
310 320 330 340 350
SVQWDQGSGR LQVYLKLRCD LPPKSQALNI HLHLPLPRGV VSLSQELSSP
360 370 380 390 400
DQKAELGEGA LHWDLPRVQG GSQLSGLFQM DVPGLQGPPS RGPSPSAPPL
410 420 430 440 450
GLGPASLSFE LPRHTCSGLQ VRFLRLSFSA CGNANPHKWV RHLSHSNAYV

IRI
Length:453
Mass (Da):49,853
Last modified:January 24, 2006 - v1
Checksum:i9605A72F134568FC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ298439 mRNA. Translation: ABC02084.1.
RefSeqiNP_001033066.1. NM_001037977.1.
UniGeneiRn.154558.

Genome annotation databases

EnsembliENSRNOT00000001827; ENSRNOP00000001827; ENSRNOG00000001353.
GeneIDi304344.
KEGGirno:304344.
UCSCiRGD:1310233. rat.

Similar proteinsi

Entry informationi

Entry nameiAP4M1_RAT
AccessioniPrimary (citable) accession number: Q2PWT8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: January 24, 2006
Last modified: November 22, 2017
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families