Q2PS28 (Q2PS28_9PSED) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Sucrose isomerase EMBL ABC33903.1

Submitted name:
Trehalulose synthase EMBL ACO05018.1

Gene names

Name:

mutB EMBL ABC33903.1

Organism

Pseudomonas mesoacidophila EMBL ABC33903.1

Taxonomic identifier

265293 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	584 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

Ontologies

Keywords
Domain	Signal EMBL ABC33903.1
Ligand	Calcium PDB 1ZJA PDB 1ZJB PDB 2PWH PDB 2PWG PDB 2PWF PDB 2PWE PDB 2PWD Metal-binding PDB 1ZJA PDB 1ZJB PDB 2PWH PDB 2PWG PDB 2PWF PDB 2PWE PDB 2PWD
Molecular function	Isomerase EMBL ABC33903.1
Technical term	3D-structure PDB 4GIN PDB 1ZJA PDB 1ZJB PDB 2PWH PDB 2PWG PDB 2PWF PDB 2PWE PDB 2PWD PDB 4GIA PDB 4H2C PDB 4GI6 PDB 4GI8 PDB 4GI9
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	isomerase activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential EMBL ABC33903.1
	Chain	24 – 584	561	sucrose isomerase EMBL ABC33903.1		PRO_5000141116
Regions
	Region	88 – 91	4	Sucrose binding PDB 2PWE
	Region	225 – 227	3	Sucrose binding PDB 2PWE
	Region	353 – 354	2	Sucrose binding PDB 2PWE
Sites
	Metal binding	49	1	Calcium PDB 1ZJA PDB 1ZJB PDB 2PWH PDB 2PWG PDB 2PWF PDB 2PWE PDB 2PWD
	Metal binding	51	1	Calcium PDB 1ZJA PDB 1ZJB PDB 2PWH PDB 2PWG PDB 2PWF PDB 2PWE PDB 2PWD
	Metal binding	53	1	Calcium PDB 1ZJA PDB 1ZJB PDB 2PWH PDB 2PWG PDB 2PWF PDB 2PWE PDB 2PWD
	Metal binding	55	1	Calcium; via carbonyl oxygen PDB 1ZJA PDB 1ZJB PDB 2PWH PDB 2PWG PDB 2PWF PDB 2PWE PDB 2PWD
	Metal binding	57	1	Calcium PDB 1ZJA PDB 1ZJB PDB 2PWH PDB 2PWG PDB 2PWF PDB 2PWE PDB 2PWD
	Binding site	131	1	Sucrose PDB 2PWE
	Binding site	191	1	Sucrose PDB 2PWE
	Binding site	283	1	Sucrose PDB 2PWE
	Binding site	441	1	Sucrose PDB 2PWE

Sequences

	Sequence		Length	Mass (Da)
	Q2PS28 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: E268C579C76CF6F7	FASTA	584	66,792
	10 20 30 40 50 60 MLMKRLFAAS LMLAFSSVSS VRAEEAVKPG APWWKSAVFY QVYPRSFKDT NGDGIGDFKG 70 80 90 100 110 120 LTEKLDYLKG LGIDAIWINP HYASPNTDNG YDISDYREVM KEYGTMEDFD RLMAELKKRG 130 140 150 160 170 180 MRLMVDVVIN HSSDQHEWFK SSRASKDNPY RDYYFWRDGK DGHEPNNYPS FFGGSAWEKD 190 200 210 220 230 240 PVTGQYYLHY FGRQQPDLNW DTPKLREELY AMLRFWLDKG VSGMRFDTVA TYSKTPGFPD 250 260 270 280 290 300 LTPEQMKNFA EAYTQGPNLH RYLQEMHEKV FDHYDAVTAG EIFGAPLNQV PLFIDSRRKE 310 320 330 340 350 360 LDMAFTFDLI RYDRALDRWH TIPRTLADFR QTIDKVDAIA GEYGWNTFFL GNHDNPRAVS 370 380 390 400 410 420 HFGDDRPQWR EASAKALATV TLTQRGTPFI FQGDELGMTN YPFKTLQDFD DIEVKGFFQD 430 440 450 460 470 480 YVETGKATAE ELLTNVALTS RDNARTPFQW DDSANAGFTT GKPWLKVNPN YTEINAAREI 490 500 510 520 530 540 GDPKSVYSFY RNLISIRHET PALSTGSYRD IDPSNADVYA YTRSQDGETY LVVVNFKAEP 550 560 570 580 RSFTLPDGMH IAETLIESSS PAAPAAGAAS LELQPWQSGI YKVK

References

[1]	"Expression, purification, crystallization and preliminary X-ray crystallographic studies of the trehalulose synthase MutB from Pseudomonas mesoacidophila MX-45." Ravaud S., Watzlawick H., Haser R., Mattes R., Aghajari N. Acta Crystallogr. F 61:100-103(2005) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 28-584 IN COMPLEX WITH CALCIUM.
[2]	"Characterization of the highly efficient sucrose isomerase from Pantoea dispersa UQ68J and cloning of the sucrose isomerase gene." Wu L., Birch R.G. Appl. Environ. Microbiol. 71:1581-1590(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: MX-45 EMBL ABC33903.1.
[3]	Goh S.L., Stiller J., Birch R.G. Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: MX-45 EMBL ABC33903.1.
[4]	"Trehalulose synthase native and carbohydrate complexed structures provide insights into sucrose isomerization." Ravaud S., Robert X., Watzlawick H., Haser R., Mattes R., Aghajari N. J. Biol. Chem. 282:28126-28136(2007) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS) OF 28-584 IN COMPLEX WITH CALCIUM AND SUCROSE.
[5]	"Gene cloning, protein characterization, and alteration of product selectivity for the trehalulose hydrolase and trehalulose synthase from "Pseudomonas mesoacidophila" MX-45." Watzlawick H., Mattes R. Appl. Environ. Microbiol. 75:7026-7036(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: MX-45 EMBL ACO05018.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

DQ304536 Genomic DNA. Translation: ABC33903.1.
FJ792641 Genomic DNA. Translation: ACO05018.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ZJA	X-ray	1.60	A/B	28-584	[»]
1ZJB	X-ray	1.80	A/B	28-584	[»]
2PWD	X-ray	1.80	A/B	28-584	[»]
2PWE	X-ray	2.00	A/B	29-584	[»]
2PWF	X-ray	1.80	A/B/C/D	29-584	[»]
2PWG	X-ray	2.20	A/B	29-584	[»]
2PWH	X-ray	2.00	A/B	29-584	[»]

ModBase

Search...

Protein family/group databases

CAZy

GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

2.60.40.1180. 1 hit.
3.20.20.80. 2 hits.

InterPro

IPR022567. DUF3459.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]

PANTHER

PTHR10357. PTHR10357. 1 hit.

Pfam

PF00128. Alpha-amylase. 1 hit.
PF11941. DUF3459. 1 hit.
[Graphical view]

SMART

SM00642. Aamy. 1 hit.
[Graphical view]

SUPFAM

SSF51445. Glyco_hydro_cat. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

Q2PS28.

Entry information

Entry name

Q2PS28_9PSED

Accession

Primary (citable) accession number: Q2PS28

Entry history

Integrated into UniProtKB/TrEMBL:	January 24, 2006
Last sequence update:	January 24, 2006
Last modified:	May 1, 2013
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)