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Q2PS28 (Q2PS28_9PSED) Unreviewed, UniProtKB/TrEMBL

Last modified March 19, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein names

Trehalulose synthase EMBL ACO05018.1
Gene names
Name:mutB EMBL ABC33903.1
OrganismPseudomonas mesoacidophila EMBL ABC33903.1
Taxonomic identifier265293 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length584 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential EMBL ABC33903.1
Chain24 – 584561sucrose isomerase EMBL ABC33903.1
PRO_5000141116

Regions

Region88 – 914Sucrose binding
Region225 – 2273Sucrose binding
Region353 – 3542Sucrose binding

Sites

Metal binding491Calcium PDB 1ZJA PDB 1ZJB PDB 2PWH PDB 2PWG PDB 2PWF PDB 2PWE PDB 2PWD PDB 4GO8 PDB 4GO9
Metal binding511Calcium PDB 1ZJA PDB 1ZJB PDB 2PWH PDB 2PWG PDB 2PWF PDB 2PWE PDB 2PWD PDB 4GO8 PDB 4GO9
Metal binding531Calcium PDB 1ZJA PDB 1ZJB PDB 2PWH PDB 2PWG PDB 2PWF PDB 2PWE PDB 2PWD PDB 4GO8 PDB 4GO9
Metal binding551Calcium; via carbonyl oxygen PDB 1ZJA PDB 1ZJB PDB 2PWH PDB 2PWG PDB 2PWF PDB 2PWE PDB 2PWD PDB 4GO8 PDB 4GO9
Metal binding571Calcium PDB 1ZJA PDB 1ZJB PDB 2PWH PDB 2PWG PDB 2PWF PDB 2PWE PDB 2PWD PDB 4GO8 PDB 4GO9
Binding site1311Sucrose
Binding site1911Sucrose
Binding site2831Sucrose
Binding site4411Sucrose

Sequences

Sequence LengthMass (Da)Tools
Q2PS28 [UniParc].

Last modified January 24, 2006. Version 1.
Checksum: E268C579C76CF6F7

FASTA58466,792
        10         20         30         40         50         60 
MLMKRLFAAS LMLAFSSVSS VRAEEAVKPG APWWKSAVFY QVYPRSFKDT NGDGIGDFKG 

        70         80         90        100        110        120 
LTEKLDYLKG LGIDAIWINP HYASPNTDNG YDISDYREVM KEYGTMEDFD RLMAELKKRG 

       130        140        150        160        170        180 
MRLMVDVVIN HSSDQHEWFK SSRASKDNPY RDYYFWRDGK DGHEPNNYPS FFGGSAWEKD 

       190        200        210        220        230        240 
PVTGQYYLHY FGRQQPDLNW DTPKLREELY AMLRFWLDKG VSGMRFDTVA TYSKTPGFPD 

       250        260        270        280        290        300 
LTPEQMKNFA EAYTQGPNLH RYLQEMHEKV FDHYDAVTAG EIFGAPLNQV PLFIDSRRKE 

       310        320        330        340        350        360 
LDMAFTFDLI RYDRALDRWH TIPRTLADFR QTIDKVDAIA GEYGWNTFFL GNHDNPRAVS 

       370        380        390        400        410        420 
HFGDDRPQWR EASAKALATV TLTQRGTPFI FQGDELGMTN YPFKTLQDFD DIEVKGFFQD 

       430        440        450        460        470        480 
YVETGKATAE ELLTNVALTS RDNARTPFQW DDSANAGFTT GKPWLKVNPN YTEINAAREI 

       490        500        510        520        530        540 
GDPKSVYSFY RNLISIRHET PALSTGSYRD IDPSNADVYA YTRSQDGETY LVVVNFKAEP 

       550        560        570        580 
RSFTLPDGMH IAETLIESSS PAAPAAGAAS LELQPWQSGI YKVK 

« Hide

References

[1]"Expression, purification, crystallization and preliminary X-ray crystallographic studies of the trehalulose synthase MutB from Pseudomonas mesoacidophila MX-45."
Ravaud S., Watzlawick H., Haser R., Mattes R., Aghajari N.
Acta Crystallogr. F 61:100-103(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 28-584 IN COMPLEX WITH CALCIUM.
[2]"Characterization of the highly efficient sucrose isomerase from Pantoea dispersa UQ68J and cloning of the sucrose isomerase gene."
Wu L., Birch R.G.
Appl. Environ. Microbiol. 71:1581-1590(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: MX-45 EMBL ABC33903.1.
[3]Goh S.L., Stiller J., Birch R.G.
Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: MX-45 EMBL ABC33903.1.
[4]"Trehalulose synthase native and carbohydrate complexed structures provide insights into sucrose isomerization."
Ravaud S., Robert X., Watzlawick H., Haser R., Mattes R., Aghajari N.
J. Biol. Chem. 282:28126-28136(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS) OF 28-584 IN COMPLEX WITH CALCIUM AND SUCROSE.
[5]"Gene cloning, protein characterization, and alteration of product selectivity for the trehalulose hydrolase and trehalulose synthase from "Pseudomonas mesoacidophila" MX-45."
Watzlawick H., Mattes R.
Appl. Environ. Microbiol. 75:7026-7036(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: MX-45 EMBL ACO05018.1.
[6]"Crystal structure of MUTB A258V mutant in complex with TRIS."
Lipski A., Watzlawick H., Ravaud S., Robert X., Haser R., Mattes R., Aghajari N.
Submitted (AUG-2012) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS) OF 28-584 IN COMPLEX WITH CALCIUM.
[7]"CRYSTAL STRUCTURE of the TREHALULOSE SYNTHASE MUTB, MUTANT D415N, in COMPLEX with TRIS."
Lipski A., Watzlawick H., Ravaud S., Robert X., Haser R., Mattes R., Aghajari N.
Submitted (AUG-2012) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) OF 28-584 IN COMPLEX WITH CALCIUM.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DQ304536 Genomic DNA. Translation: ABC33903.1.
FJ792641 Genomic DNA. Translation: ACO05018.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZJAX-ray1.60A/B28-584[»]
1ZJBX-ray1.80A/B28-584[»]
2PWDX-ray1.80A/B28-584[»]
2PWEX-ray2.00A/B29-584[»]
2PWFX-ray1.80A/B/C/D29-584[»]
2PWGX-ray2.20A/B29-584[»]
2PWHX-ray2.00A/B29-584[»]
4GO8X-ray2.15A/B28-584[»]
4GO9X-ray2.20A/B28-584[»]
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.60.40.1180. 1 hit.
3.20.20.80. 2 hits.
InterProIPR022567. DUF3459.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF11941. DUF3459. 1 hit.
[Graphical view]
SMARTSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceQ2PS28.

Entry information

Entry nameQ2PS28_9PSED
AccessionPrimary (citable) accession number: Q2PS28
Entry history
Integrated into UniProtKB/TrEMBL: January 24, 2006
Last sequence update: January 24, 2006
Last modified: March 19, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)