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Q2PS28

- Q2PS28_9PSED

UniProt

Q2PS28 - Q2PS28_9PSED

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Protein
Submitted name: Sucrose isomerase
Gene
mutB
Organism
Pseudomonas mesoacidophila
Status
Unreviewed - Annotation score: 2 out of 5 - Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi49 – 491CalciumImported
Metal bindingi51 – 511CalciumImported
Metal bindingi53 – 531CalciumImported
Metal bindingi55 – 551Calcium; via carbonyl oxygenImported
Metal bindingi57 – 571CalciumImported
Binding sitei131 – 1311Sucrose
Binding sitei191 – 1911Sucrose
Binding sitei283 – 2831Sucrose
Binding sitei441 – 4411Sucrose

GO - Molecular functioni

  1. isomerase activity Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

IsomeraseImported

Keywords - Ligandi

CalciumImported, Metal-bindingImported

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Submitted name:
Sucrose isomeraseImported
Submitted name:
Trehalulose synthaseImported
Gene namesi
Name:mutBImported
OrganismiPseudomonas mesoacidophilaImported
Taxonomic identifieri265293 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323 PredictedImported
Add
BLAST
Chaini24 – 584561sucrose isomeraseImported
PRO_5000141116Add
BLAST

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZJAX-ray1.60A/B28-584[»]
1ZJBX-ray1.80A/B28-584[»]
2PWDX-ray1.80A/B28-584[»]
2PWEX-ray2.00A/B29-584[»]
2PWFX-ray1.80A/B/C/D29-584[»]
2PWGX-ray2.20A/B29-584[»]
2PWHX-ray2.00A/B29-584[»]
4GO8X-ray2.15A/B28-584[»]
4GO9X-ray2.20A/B28-584[»]

Miscellaneous databases

EvolutionaryTraceiQ2PS28.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni88 – 914Sucrose binding
Regioni225 – 2273Sucrose binding
Regioni353 – 3542Sucrose binding

Keywords - Domaini

SignalImported

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR022567. DUF3459.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF11941. DUF3459. 1 hit.
[Graphical view]
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q2PS28-1 [UniParc]FASTAAdd to Basket

« Hide

MLMKRLFAAS LMLAFSSVSS VRAEEAVKPG APWWKSAVFY QVYPRSFKDT    50
NGDGIGDFKG LTEKLDYLKG LGIDAIWINP HYASPNTDNG YDISDYREVM 100
KEYGTMEDFD RLMAELKKRG MRLMVDVVIN HSSDQHEWFK SSRASKDNPY 150
RDYYFWRDGK DGHEPNNYPS FFGGSAWEKD PVTGQYYLHY FGRQQPDLNW 200
DTPKLREELY AMLRFWLDKG VSGMRFDTVA TYSKTPGFPD LTPEQMKNFA 250
EAYTQGPNLH RYLQEMHEKV FDHYDAVTAG EIFGAPLNQV PLFIDSRRKE 300
LDMAFTFDLI RYDRALDRWH TIPRTLADFR QTIDKVDAIA GEYGWNTFFL 350
GNHDNPRAVS HFGDDRPQWR EASAKALATV TLTQRGTPFI FQGDELGMTN 400
YPFKTLQDFD DIEVKGFFQD YVETGKATAE ELLTNVALTS RDNARTPFQW 450
DDSANAGFTT GKPWLKVNPN YTEINAAREI GDPKSVYSFY RNLISIRHET 500
PALSTGSYRD IDPSNADVYA YTRSQDGETY LVVVNFKAEP RSFTLPDGMH 550
IAETLIESSS PAAPAAGAAS LELQPWQSGI YKVK 584
Length:584
Mass (Da):66,792
Last modified:January 24, 2006 - v1
Checksum:iE268C579C76CF6F7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ304536 Genomic DNA. Translation: ABC33903.1.
FJ792641 Genomic DNA. Translation: ACO05018.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ304536 Genomic DNA. Translation: ABC33903.1 .
FJ792641 Genomic DNA. Translation: ACO05018.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1ZJA X-ray 1.60 A/B 28-584 [» ]
1ZJB X-ray 1.80 A/B 28-584 [» ]
2PWD X-ray 1.80 A/B 28-584 [» ]
2PWE X-ray 2.00 A/B 29-584 [» ]
2PWF X-ray 1.80 A/B/C/D 29-584 [» ]
2PWG X-ray 2.20 A/B 29-584 [» ]
2PWH X-ray 2.00 A/B 29-584 [» ]
4GO8 X-ray 2.15 A/B 28-584 [» ]
4GO9 X-ray 2.20 A/B 28-584 [» ]
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei Q2PS28.

Family and domain databases

Gene3Di 2.60.40.1180. 1 hit.
3.20.20.80. 2 hits.
InterProi IPR022567. DUF3459.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10357. PTHR10357. 1 hit.
Pfami PF00128. Alpha-amylase. 1 hit.
PF11941. DUF3459. 1 hit.
[Graphical view ]
SMARTi SM00642. Aamy. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Expression, purification, crystallization and preliminary X-ray crystallographic studies of the trehalulose synthase MutB from Pseudomonas mesoacidophila MX-45."
    Ravaud S., Watzlawick H., Haser R., Mattes R., Aghajari N.
    Acta Crystallogr. F 61:100-103(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 28-584 IN COMPLEX WITH CALCIUM.
  2. "Characterization of the highly efficient sucrose isomerase from Pantoea dispersa UQ68J and cloning of the sucrose isomerase gene."
    Wu L., Birch R.G.
    Appl. Environ. Microbiol. 71:1581-1590(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: MX-45Imported.
  3. Goh S.L., Stiller J., Birch R.G.
    Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: MX-45Imported.
  4. "Trehalulose synthase native and carbohydrate complexed structures provide insights into sucrose isomerization."
    Ravaud S., Robert X., Watzlawick H., Haser R., Mattes R., Aghajari N.
    J. Biol. Chem. 282:28126-28136(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS) OF 28-584 IN COMPLEX WITH CALCIUM AND SUCROSE.
  5. "Gene cloning, protein characterization, and alteration of product selectivity for the trehalulose hydrolase and trehalulose synthase from "Pseudomonas mesoacidophila" MX-45."
    Watzlawick H., Mattes R.
    Appl. Environ. Microbiol. 75:7026-7036(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: MX-45Imported.
  6. "Crystal structure of MUTB A258V mutant in complex with TRIS."
    Lipski A., Watzlawick H., Ravaud S., Robert X., Haser R., Mattes R., Aghajari N.
    Submitted (AUG-2012) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS) OF 28-584 IN COMPLEX WITH CALCIUM.
  7. "CRYSTAL STRUCTURE of the TREHALULOSE SYNTHASE MUTB, MUTANT D415N, in COMPLEX with TRIS."
    Lipski A., Watzlawick H., Ravaud S., Robert X., Haser R., Mattes R., Aghajari N.
    Submitted (AUG-2012) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) OF 28-584 IN COMPLEX WITH CALCIUM.
  8. Watzlawick H., Mattes R.
    Submitted (JUN-2014) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: MX-45Imported.

Entry informationi

Entry nameiQ2PS28_9PSED
AccessioniPrimary (citable) accession number: Q2PS28
Entry historyi
Integrated into UniProtKB/TrEMBL: January 24, 2006
Last sequence update: January 24, 2006
Last modified: September 3, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureImported

External Data

Dasty 3

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