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Q2PS28

- Q2PS28_9PSED

UniProt

Q2PS28 - Q2PS28_9PSED

Protein
Submitted name:

Sucrose isomerase

Gene

mutB

Organism
Pseudomonas mesoacidophila
Status
Unreviewed - Annotation score: 1 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 57 (01 Oct 2014)
      Sequence version 1 (24 Jan 2006)
      Previous versions | rss
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    Functioni

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi49 – 491CalciumImported
    Metal bindingi51 – 511CalciumImported
    Metal bindingi53 – 531CalciumImported
    Metal bindingi55 – 551Calcium; via carbonyl oxygenImported
    Metal bindingi57 – 571CalciumImported
    Binding sitei131 – 1311Sucrose
    Binding sitei191 – 1911Sucrose
    Binding sitei283 – 2831Sucrose
    Binding sitei441 – 4411Sucrose

    GO - Molecular functioni

    1. isomerase activity Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    IsomeraseImported

    Keywords - Ligandi

    CalciumImported, Metal-bindingImported

    Protein family/group databases

    CAZyiGH13. Glycoside Hydrolase Family 13.

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    Sucrose isomeraseImported
    Submitted name:
    Trehalulose synthaseImported
    Gene namesi
    Name:mutBImported
    OrganismiPseudomonas mesoacidophilaImported
    Taxonomic identifieri265293 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323 PotentialImportedAdd
    BLAST
    Chaini24 – 584561sucrose isomeraseImportedPRO_5000141116Add
    BLAST

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1ZJAX-ray1.60A/B28-584[»]
    1ZJBX-ray1.80A/B28-584[»]
    2PWDX-ray1.80A/B28-584[»]
    2PWEX-ray2.00A/B29-584[»]
    2PWFX-ray1.80A/B/C/D29-584[»]
    2PWGX-ray2.20A/B29-584[»]
    2PWHX-ray2.00A/B29-584[»]
    4GO8X-ray2.15A/B28-584[»]
    4GO9X-ray2.20A/B28-584[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ2PS28.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni88 – 914Sucrose binding
    Regioni225 – 2273Sucrose binding
    Regioni353 – 3542Sucrose binding

    Keywords - Domaini

    SignalImported

    Family and domain databases

    Gene3Di2.60.40.1180. 1 hit.
    3.20.20.80. 2 hits.
    InterProiIPR022567. DUF3459.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR006589. Glyco_hydro_13_sub_cat_dom.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10357. PTHR10357. 1 hit.
    PfamiPF00128. Alpha-amylase. 1 hit.
    PF11941. DUF3459. 1 hit.
    [Graphical view]
    SMARTiSM00642. Aamy. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q2PS28-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLMKRLFAAS LMLAFSSVSS VRAEEAVKPG APWWKSAVFY QVYPRSFKDT    50
    NGDGIGDFKG LTEKLDYLKG LGIDAIWINP HYASPNTDNG YDISDYREVM 100
    KEYGTMEDFD RLMAELKKRG MRLMVDVVIN HSSDQHEWFK SSRASKDNPY 150
    RDYYFWRDGK DGHEPNNYPS FFGGSAWEKD PVTGQYYLHY FGRQQPDLNW 200
    DTPKLREELY AMLRFWLDKG VSGMRFDTVA TYSKTPGFPD LTPEQMKNFA 250
    EAYTQGPNLH RYLQEMHEKV FDHYDAVTAG EIFGAPLNQV PLFIDSRRKE 300
    LDMAFTFDLI RYDRALDRWH TIPRTLADFR QTIDKVDAIA GEYGWNTFFL 350
    GNHDNPRAVS HFGDDRPQWR EASAKALATV TLTQRGTPFI FQGDELGMTN 400
    YPFKTLQDFD DIEVKGFFQD YVETGKATAE ELLTNVALTS RDNARTPFQW 450
    DDSANAGFTT GKPWLKVNPN YTEINAAREI GDPKSVYSFY RNLISIRHET 500
    PALSTGSYRD IDPSNADVYA YTRSQDGETY LVVVNFKAEP RSFTLPDGMH 550
    IAETLIESSS PAAPAAGAAS LELQPWQSGI YKVK 584
    Length:584
    Mass (Da):66,792
    Last modified:January 24, 2006 - v1
    Checksum:iE268C579C76CF6F7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ304536 Genomic DNA. Translation: ABC33903.1.
    FJ792641 Genomic DNA. Translation: ACO05018.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ304536 Genomic DNA. Translation: ABC33903.1 .
    FJ792641 Genomic DNA. Translation: ACO05018.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1ZJA X-ray 1.60 A/B 28-584 [» ]
    1ZJB X-ray 1.80 A/B 28-584 [» ]
    2PWD X-ray 1.80 A/B 28-584 [» ]
    2PWE X-ray 2.00 A/B 29-584 [» ]
    2PWF X-ray 1.80 A/B/C/D 29-584 [» ]
    2PWG X-ray 2.20 A/B 29-584 [» ]
    2PWH X-ray 2.00 A/B 29-584 [» ]
    4GO8 X-ray 2.15 A/B 28-584 [» ]
    4GO9 X-ray 2.20 A/B 28-584 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH13. Glycoside Hydrolase Family 13.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei Q2PS28.

    Family and domain databases

    Gene3Di 2.60.40.1180. 1 hit.
    3.20.20.80. 2 hits.
    InterProi IPR022567. DUF3459.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR006589. Glyco_hydro_13_sub_cat_dom.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10357. PTHR10357. 1 hit.
    Pfami PF00128. Alpha-amylase. 1 hit.
    PF11941. DUF3459. 1 hit.
    [Graphical view ]
    SMARTi SM00642. Aamy. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Expression, purification, crystallization and preliminary X-ray crystallographic studies of the trehalulose synthase MutB from Pseudomonas mesoacidophila MX-45."
      Ravaud S., Watzlawick H., Haser R., Mattes R., Aghajari N.
      Acta Crystallogr. F 61:100-103(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 28-584 IN COMPLEX WITH CALCIUM.
    2. "Characterization of the highly efficient sucrose isomerase from Pantoea dispersa UQ68J and cloning of the sucrose isomerase gene."
      Wu L., Birch R.G.
      Appl. Environ. Microbiol. 71:1581-1590(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: MX-45Imported.
    3. Goh S.L., Stiller J., Birch R.G.
      Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: MX-45Imported.
    4. "Trehalulose synthase native and carbohydrate complexed structures provide insights into sucrose isomerization."
      Ravaud S., Robert X., Watzlawick H., Haser R., Mattes R., Aghajari N.
      J. Biol. Chem. 282:28126-28136(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS) OF 28-584 IN COMPLEX WITH CALCIUM.
    5. "Gene cloning, protein characterization, and alteration of product selectivity for the trehalulose hydrolase and trehalulose synthase from "Pseudomonas mesoacidophila" MX-45."
      Watzlawick H., Mattes R.
      Appl. Environ. Microbiol. 75:7026-7036(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: MX-45Imported.
    6. "Crystal structure of MUTB A258V mutant in complex with TRIS."
      Lipski A., Watzlawick H., Ravaud S., Robert X., Haser R., Mattes R., Aghajari N.
      Submitted (AUG-2012) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS) OF 28-584 IN COMPLEX WITH CALCIUM.
    7. "CRYSTAL STRUCTURE of the TREHALULOSE SYNTHASE MUTB, MUTANT D415N, in COMPLEX with TRIS."
      Lipski A., Watzlawick H., Ravaud S., Robert X., Haser R., Mattes R., Aghajari N.
      Submitted (AUG-2012) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) OF 28-584 IN COMPLEX WITH CALCIUM.
    8. Watzlawick H., Mattes R.
      Submitted (JUN-2014) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: MX-45Imported.

    Entry informationi

    Entry nameiQ2PS28_9PSED
    AccessioniPrimary (citable) accession number: Q2PS28
    Entry historyi
    Integrated into UniProtKB/TrEMBL: January 24, 2006
    Last sequence update: January 24, 2006
    Last modified: October 1, 2014
    This is version 57 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported

    External Data

    Dasty 3