Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q2PS28

- Q2PS28_9PSED

UniProt

Q2PS28 - Q2PS28_9PSED

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Submitted name:

Sucrose isomerase

Gene

mutB

Organism
Pseudomonas mesoacidophila
Status
Unreviewed - Annotation score: 1 out of 5- Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi49 – 491CalciumImported
Metal bindingi51 – 511CalciumImported
Metal bindingi53 – 531CalciumImported
Metal bindingi55 – 551Calcium; via carbonyl oxygenImported
Metal bindingi57 – 571CalciumImported

GO - Molecular functioni

  1. isomerase activity Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

IsomeraseImported

Keywords - Ligandi

CalciumImported, Metal-bindingImported

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Submitted name:
Sucrose isomeraseImported
Submitted name:
Trehalulose synthaseImported
Gene namesi
Name:mutBImported
OrganismiPseudomonas mesoacidophilaImported
Taxonomic identifieri265293 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323 PotentialImportedAdd
BLAST
Chaini24 – 584561sucrose isomeraseImportedPRO_5000141116Add
BLAST

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZJAX-ray1.60A/B28-584[»]
1ZJBX-ray1.80A/B28-584[»]
2PWDX-ray1.80A/B28-584[»]
2PWEX-ray2.00A/B29-584[»]
2PWFX-ray1.80A/B/C/D29-584[»]
2PWGX-ray2.20A/B29-584[»]
2PWHX-ray2.00A/B29-584[»]
4GO8X-ray2.15A/B28-584[»]
4GO9X-ray2.20A/B28-584[»]
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ2PS28.

Family & Domainsi

Keywords - Domaini

SignalImported

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR022567. DUF3459.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF11941. DUF3459. 1 hit.
[Graphical view]
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q2PS28-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLMKRLFAAS LMLAFSSVSS VRAEEAVKPG APWWKSAVFY QVYPRSFKDT
60 70 80 90 100
NGDGIGDFKG LTEKLDYLKG LGIDAIWINP HYASPNTDNG YDISDYREVM
110 120 130 140 150
KEYGTMEDFD RLMAELKKRG MRLMVDVVIN HSSDQHEWFK SSRASKDNPY
160 170 180 190 200
RDYYFWRDGK DGHEPNNYPS FFGGSAWEKD PVTGQYYLHY FGRQQPDLNW
210 220 230 240 250
DTPKLREELY AMLRFWLDKG VSGMRFDTVA TYSKTPGFPD LTPEQMKNFA
260 270 280 290 300
EAYTQGPNLH RYLQEMHEKV FDHYDAVTAG EIFGAPLNQV PLFIDSRRKE
310 320 330 340 350
LDMAFTFDLI RYDRALDRWH TIPRTLADFR QTIDKVDAIA GEYGWNTFFL
360 370 380 390 400
GNHDNPRAVS HFGDDRPQWR EASAKALATV TLTQRGTPFI FQGDELGMTN
410 420 430 440 450
YPFKTLQDFD DIEVKGFFQD YVETGKATAE ELLTNVALTS RDNARTPFQW
460 470 480 490 500
DDSANAGFTT GKPWLKVNPN YTEINAAREI GDPKSVYSFY RNLISIRHET
510 520 530 540 550
PALSTGSYRD IDPSNADVYA YTRSQDGETY LVVVNFKAEP RSFTLPDGMH
560 570 580
IAETLIESSS PAAPAAGAAS LELQPWQSGI YKVK
Length:584
Mass (Da):66,792
Last modified:January 24, 2006 - v1
Checksum:iE268C579C76CF6F7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ304536 Genomic DNA. Translation: ABC33903.1.
FJ792641 Genomic DNA. Translation: ACO05018.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ304536 Genomic DNA. Translation: ABC33903.1 .
FJ792641 Genomic DNA. Translation: ACO05018.1 .

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1ZJA X-ray 1.60 A/B 28-584 [» ]
1ZJB X-ray 1.80 A/B 28-584 [» ]
2PWD X-ray 1.80 A/B 28-584 [» ]
2PWE X-ray 2.00 A/B 29-584 [» ]
2PWF X-ray 1.80 A/B/C/D 29-584 [» ]
2PWG X-ray 2.20 A/B 29-584 [» ]
2PWH X-ray 2.00 A/B 29-584 [» ]
4GO8 X-ray 2.15 A/B 28-584 [» ]
4GO9 X-ray 2.20 A/B 28-584 [» ]
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei Q2PS28.

Family and domain databases

Gene3Di 2.60.40.1180. 1 hit.
3.20.20.80. 2 hits.
InterProi IPR022567. DUF3459.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10357. PTHR10357. 1 hit.
Pfami PF00128. Alpha-amylase. 1 hit.
PF11941. DUF3459. 1 hit.
[Graphical view ]
SMARTi SM00642. Aamy. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Expression, purification, crystallization and preliminary X-ray crystallographic studies of the trehalulose synthase MutB from Pseudomonas mesoacidophila MX-45."
    Ravaud S., Watzlawick H., Haser R., Mattes R., Aghajari N.
    Acta Crystallogr. F 61:100-103(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 28-584 IN COMPLEX WITH CALCIUM.
  2. "Characterization of the highly efficient sucrose isomerase from Pantoea dispersa UQ68J and cloning of the sucrose isomerase gene."
    Wu L., Birch R.G.
    Appl. Environ. Microbiol. 71:1581-1590(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: MX-45Imported.
  3. Goh S.L., Stiller J., Birch R.G.
    Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: MX-45Imported.
  4. "Trehalulose synthase native and carbohydrate complexed structures provide insights into sucrose isomerization."
    Ravaud S., Robert X., Watzlawick H., Haser R., Mattes R., Aghajari N.
    J. Biol. Chem. 282:28126-28136(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS) OF 28-584 IN COMPLEX WITH CALCIUM.
  5. "Gene cloning, protein characterization, and alteration of product selectivity for the trehalulose hydrolase and trehalulose synthase from "Pseudomonas mesoacidophila" MX-45."
    Watzlawick H., Mattes R.
    Appl. Environ. Microbiol. 75:7026-7036(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: MX-45Imported.
  6. "Crystal structure of MUTB A258V mutant in complex with TRIS."
    Lipski A., Watzlawick H., Ravaud S., Robert X., Haser R., Mattes R., Aghajari N.
    Submitted (AUG-2012) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS) OF 28-584 IN COMPLEX WITH CALCIUM.
  7. "CRYSTAL STRUCTURE of the TREHALULOSE SYNTHASE MUTB, MUTANT D415N, in COMPLEX with TRIS."
    Lipski A., Watzlawick H., Ravaud S., Robert X., Haser R., Mattes R., Aghajari N.
    Submitted (AUG-2012) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) OF 28-584 IN COMPLEX WITH CALCIUM.
  8. Watzlawick H., Mattes R.
    Submitted (JUN-2014) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: MX-45Imported.

Entry informationi

Entry nameiQ2PS28_9PSED
AccessioniPrimary (citable) accession number: Q2PS28
Entry historyi
Integrated into UniProtKB/TrEMBL: January 24, 2006
Last sequence update: January 24, 2006
Last modified: November 26, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureImported

External Data

Dasty 3