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Protein

Junctophilin-2

Gene

Jph2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Junctophilins contribute to the formation of junctional membrane complexes (JMCs) which link the plasma membrane with the endoplasmic or sarcoplasmic reticulum in excitable cells. Provides a structural foundation for functional cross-talk between the cell surface and intracellular calcium release channels. JPH2 is necessary for proper intracellular Ca2+ signaling in cardiac myocytes via its involvement in ryanodine receptor-mediated calcium ion release. Contributes to the construction of skeletal muscle triad junctions (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Names & Taxonomyi

Protein namesi
Recommended name:
Junctophilin-2
Short name:
JP-2
Alternative name(s):
Junctophilin type 2
Gene namesi
Name:Jph2Imported
Synonyms:Jp2By similarity
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi1305196. Jph2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 670CytoplasmicSequence analysisAdd BLAST670
Transmembranei671 – 691Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane, Sarcoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002594031 – 692Junctophilin-2Add BLAST692

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei162PhosphoserineCombined sources1
Modified residuei165PhosphoserineBy similarity1
Modified residuei440PhosphoserineCombined sources1
Modified residuei442PhosphoserineCombined sources1
Modified residuei462PhosphoserineCombined sources1
Modified residuei470PhosphothreonineBy similarity1
Modified residuei479PhosphoserineCombined sources1
Modified residuei483PhosphothreonineCombined sources1
Modified residuei527PhosphoserineCombined sources1
Modified residuei533PhosphoserineCombined sources1
Modified residuei589PhosphoserineBy similarity1
Modified residuei593PhosphoserineCombined sources1
Modified residuei604PhosphoserineCombined sources1
Modified residuei609PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation on Ser-165, probably by PKC, affects RYR1-mediated calcium ion release, interaction with TRPC3, and skeletal muscle myotubule development.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ2PS20.
PRIDEiQ2PS20.

PTM databases

iPTMnetiQ2PS20.
PhosphoSitePlusiQ2PS20.

Expressioni

Gene expression databases

BgeeiENSRNOG00000008170.

Interactioni

Protein-protein interaction databases

IntActiQ2PS20. 2 interactors.
STRINGi10116.ENSRNOP00000010938.

Structurei

3D structure databases

ProteinModelPortaliQ2PS20.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati14 – 36MORN 1Sequence analysisAdd BLAST23
Repeati38 – 59MORN 2Sequence analysisAdd BLAST22
Repeati60 – 79MORN 3Sequence analysisAdd BLAST20
Repeati82 – 104MORN 4Sequence analysisAdd BLAST23
Repeati106 – 128MORN 5Sequence analysisAdd BLAST23
Repeati129 – 151MORN 6Sequence analysisAdd BLAST23
Repeati285 – 307MORN 7Sequence analysisAdd BLAST23
Repeati308 – 330MORN 8Sequence analysisAdd BLAST23

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi3 – 142Gly-richSequence analysisAdd BLAST140
Compositional biasi367 – 402Ala-richSequence analysisAdd BLAST36
Compositional biasi446 – 629Pro-richSequence analysisAdd BLAST184

Domaini

The MORN (membrane occupation and recognition nexus) repeats contribute to the plasma membrane binding, by interacting with phospholipids. Has affinity for phosphatidylserine, and phosphorylated phosphatidylinositols including PtdIns3P, PtdIns4P, PtdIns5P, PtdIns(3,5)P2 and PtdIns(3,4,5)P3 (By similarity).By similarity

Sequence similaritiesi

Belongs to the junctophilin family.Sequence analysis
Contains 8 MORN repeats.Sequence analysis

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0231. Eukaryota.
COG4642. LUCA.
GeneTreeiENSGT00730000110639.
HOGENOMiHOG000264244.
HOVERGENiHBG031648.
InParanoidiQ2PS20.
KOiK19530.
OMAiHERETPR.
OrthoDBiEOG091G03WX.
PhylomeDBiQ2PS20.
TreeFamiTF317210.

Family and domain databases

InterProiIPR017191. Junctophilin.
IPR003409. MORN.
[Graphical view]
PfamiPF02493. MORN. 8 hits.
[Graphical view]
PIRSFiPIRSF037387. Junctophilin. 1 hit.
SMARTiSM00698. MORN. 6 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2PS20-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGGRFDFDD GGAYCGGWEG GKAHGHGLCT GPKGQGEYSG SWNFGFEVAG
60 70 80 90 100
VYTWPSGNTF EGYWSQGKRH GLGIETKGRW LYKGEWTHGF KGRYGIRQST
110 120 130 140 150
NSGAKYEGTW NNGLQDGYGT ETYADGGTYQ GQFTNGMRHG YGVRQSVPYG
160 170 180 190 200
MAVVVRSPLR TSLSSLRSEH SNGTVAPDSP AADGPTLPLP PVPRGGFALS
210 220 230 240 250
LLATAEAARP PGLFTRGALL GRLRRSESRT SLGSQRSRLS FLKSELSSGA
260 270 280 290 300
SDAASTGSLA EGAEGPDDAA APFDADIDAT TTETYMGEWK NDKRSGFGVS
310 320 330 340 350
ERSSGLRYEG EWLDNLRHGY GRTTLPDGHR EEGKYRHNVL VKGTKRRVLP
360 370 380 390 400
LKSNKVRQKV EHGVEGAQRA AAIARQKAEI AASRTSHAKA KAEAAEQAAL
410 420 430 440 450
AANQESNIAR TLAKELAPDF YQPGPEYQKR RLLQEILENS ESLLEPRERG
460 470 480 490 500
PGTGLPERPR ESPQLHERET PQPEGGPPSP AGTPPQPKRP RPGSSKDGLL
510 520 530 540 550
SPGAWNGEPG GEGSRPATPS DGAGRRSPAR PASEHMAIEA LQPPPAPSRE
560 570 580 590 600
PEVALYRGYH SYAVRTGPPE PPPLEDEPEP EPEVPRSDSE PPSPVSATVQ
610 620 630 640 650
EEESPAPRSR VPAKPATLEP KPIVPKAEPK AKARKTEARG LSKAGAKKKG
660 670 680 690
RKEVAQEAEA EVEVEEVPNT VLICMVILLN IGLAILFVHL LT
Length:692
Mass (Da):74,259
Last modified:January 24, 2006 - v1
Checksum:iB24055782462322F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ304564 mRNA. Translation: ABC02402.1.
RefSeqiNP_001033063.1. NM_001037974.1.
XP_006235588.1. XM_006235526.3.
UniGeneiRn.6206.

Genome annotation databases

EnsembliENSRNOT00000010939; ENSRNOP00000010938; ENSRNOG00000008170.
GeneIDi296345.
KEGGirno:296345.
UCSCiRGD:1305196. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ304564 mRNA. Translation: ABC02402.1.
RefSeqiNP_001033063.1. NM_001037974.1.
XP_006235588.1. XM_006235526.3.
UniGeneiRn.6206.

3D structure databases

ProteinModelPortaliQ2PS20.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ2PS20. 2 interactors.
STRINGi10116.ENSRNOP00000010938.

PTM databases

iPTMnetiQ2PS20.
PhosphoSitePlusiQ2PS20.

Proteomic databases

PaxDbiQ2PS20.
PRIDEiQ2PS20.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000010939; ENSRNOP00000010938; ENSRNOG00000008170.
GeneIDi296345.
KEGGirno:296345.
UCSCiRGD:1305196. rat.

Organism-specific databases

CTDi57158.
RGDi1305196. Jph2.

Phylogenomic databases

eggNOGiKOG0231. Eukaryota.
COG4642. LUCA.
GeneTreeiENSGT00730000110639.
HOGENOMiHOG000264244.
HOVERGENiHBG031648.
InParanoidiQ2PS20.
KOiK19530.
OMAiHERETPR.
OrthoDBiEOG091G03WX.
PhylomeDBiQ2PS20.
TreeFamiTF317210.

Miscellaneous databases

PROiQ2PS20.

Gene expression databases

BgeeiENSRNOG00000008170.

Family and domain databases

InterProiIPR017191. Junctophilin.
IPR003409. MORN.
[Graphical view]
PfamiPF02493. MORN. 8 hits.
[Graphical view]
PIRSFiPIRSF037387. Junctophilin. 1 hit.
SMARTiSM00698. MORN. 6 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiJPH2_RAT
AccessioniPrimary (citable) accession number: Q2PS20
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: January 24, 2006
Last modified: November 2, 2016
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.