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Q2PQJ3

- VSP1_BOTJR

UniProt

Q2PQJ3 - VSP1_BOTJR

Protein

Thrombin-like enzyme BjussuSP-1

Gene
N/A
Organism
Bothrops jararacussu (Jararacussu)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 38 (01 Oct 2014)
      Sequence version 1 (24 Jan 2006)
      Previous versions | rss
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    Functioni

    Thrombin-like enzyme that shows clotting activity upon human plasma. Shows specific fibrinogenolytic activity for Aalpha chain (FGA). Hydrolyzes fibrin, BAPNA and TAME, as well as chromogenic artificial substrates of the blood coagulation cascasde: S-27654 for factor X (F10), S-2302 for kallikrein (KLK), factor XIa (F11), and XIIa (F12), and S-2266 for kallikrein and factor XIa (F11). Subcutaneous injection into mice induces a mild edema. Intravenous and intramuscular injection reduce plasma fibrinogen concentration and increase the levels of fibrin(ogen) degradation products. Intramuscular injection also promotes an increase in the expression of proMMP-9, but is unable to activate it.3 Publications

    Enzyme regulationi

    Inhibited by leupeptin, heparin, and 1.10-phenantroline.2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei40 – 401Charge relay systemBy similarity
    Active sitei85 – 851Charge relay systemBy similarity
    Active sitei178 – 1781Charge relay systemBy similarity

    GO - Molecular functioni

    1. serine-type endopeptidase activity Source: InterPro

    Keywords - Molecular functioni

    Blood coagulation cascade activating toxin, Fibrinolytic toxin, Hemostasis impairing toxin, Hydrolase, Protease, Serine protease, Toxin

    Keywords - Ligandi

    Sialic acid

    Protein family/group databases

    MEROPSiS01.023.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thrombin-like enzyme BjussuSP-1 (EC:3.4.21.-)
    Short name:
    SVTLE BjussuSP-1
    Alternative name(s):
    Fibrinogen-clotting enzyme
    Jararacussin-I
    Snake venom serine protease 1
    Short name:
    SVSP
    Thrombin-like enzyme BjussuSP-I
    Short name:
    SVTLE BjussuSP-I
    OrganismiBothrops jararacussu (Jararacussu)
    Taxonomic identifieri8726 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeCrotalinaeBothrops

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 232232Thrombin-like enzyme BjussuSP-1PRO_0000296362Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi7 ↔ 1381 PublicationPROSITE-ProRule annotation
    Disulfide bondi25 ↔ 411 PublicationPROSITE-ProRule annotation
    Disulfide bondi73 ↔ 2301 PublicationPROSITE-ProRule annotation
    Glycosylationi77 – 771N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi117 ↔ 1841 PublicationPROSITE-ProRule annotation
    Glycosylationi129 – 1291N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi149 ↔ 1631 PublicationPROSITE-ProRule annotation
    Disulfide bondi174 ↔ 1991 PublicationPROSITE-ProRule annotation

    Post-translational modificationi

    N-glycosylated. Contains sialic acid residues. Deglycosylation reduces in 50% the formation of fibrin clot.2 Publications

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Expressioni

    Tissue specificityi

    Expressed by the venom gland.

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Structurei

    Secondary structure

    1
    232
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni9 – 113
    Beta strandi15 – 2915
    Beta strandi31 – 377
    Helixi39 – 413
    Beta strandi47 – 515
    Beta strandi63 – 653
    Beta strandi67 – 726
    Beta strandi87 – 937
    Beta strandi116 – 1238
    Beta strandi126 – 1294
    Beta strandi137 – 1437
    Helixi147 – 1504
    Beta strandi161 – 1655
    Beta strandi181 – 1844
    Beta strandi187 – 1948
    Beta strandi206 – 2105
    Helixi211 – 2144
    Helixi215 – 2239

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4GSOX-ray2.60A1-232[»]
    ProteinModelPortaliQ2PQJ3.
    SMRiQ2PQJ3. Positions 1-232.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 223223Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family. Snake venom subfamily.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Phylogenomic databases

    HOVERGENiHBG013304.

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q2PQJ3-1 [UniParc]FASTAAdd to Basket

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    VLGGDECDIN EHPFLAFLYS HGYFCGLTLI NQEWVVTAAH CDSTNFQMQL    50
    GVHSKKVLNE DEQTRNPKEK FICPNKNMSE VLDKDIMLIK LDKPISNSKH 100
    IAPLSLPSNP PSVGSVCRIM GWGSITIPNE TYPDVPYCAN INLVDYEVCQ 150
    GAYNGLPAKT TLCAGVLEGG KDTCVGDSGG PLICNGQFQG IVSYGAHSCG 200
    QGPKPGIYTN VFDYTDWIQR NIAGNTDATC PP 232
    Length:232
    Mass (Da):25,163
    Last modified:January 24, 2006 - v1
    Checksum:i14C6B2DE6A97064D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ307451 mRNA. Translation: ABC24687.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ307451 mRNA. Translation: ABC24687.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4GSO X-ray 2.60 A 1-232 [» ]
    ProteinModelPortali Q2PQJ3.
    SMRi Q2PQJ3. Positions 1-232.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi S01.023.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    HOVERGENi HBG013304.

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization of BjussuSP-I, a new thrombin-like enzyme with procoagulant and kallikrein-like activity isolated from Bothrops jararacussu snake venom."
      Sant'Ana C.D., Bernardes C.P., Izidoro L.F., Mazzi M.V., Soares S.G., Fuly A.L., Zingali R.B., Magro A.J., Braz A.S., Fontes M.R., Stabeli R.G., Sampaio S.V., Soares A.M.
      Biochimie 90:500-507(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME REGULATION, GLYCOSYLATION.
      Tissue: Venom gland.
    2. Cited for: PROTEIN SEQUENCE OF 1-20, FUNCTION, SUBUNIT, ENZYME REGULATION, GLYCOSYLATION, SIALIC ACID.
      Tissue: Venom.
    3. "Local and systemic pathophysiological alterations induced by a serine proteinase from the venom of the snake Bothrops jararacussu."
      Perez A.V., Saravia P., Rucavado A., Sant'Ana C.D., Soares A.M., Gutierrez J.M.
      Toxicon 49:1063-1069(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
      Tissue: Venom.
    4. "Crystal structure of jararacussin-I: the highly negatively charged catalytic interface contributes to macromolecular selectivity in snake venom thrombin-like enzymes."
      Ullah A., Souza T.A., Zanphorlin L.M., Mariutti R.B., Santana V.S., Murakami M.T., Arni R.K.
      Protein Sci. 22:128-132(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS), DISULFIDE BONDS.
      Tissue: Venom.

    Entry informationi

    Entry nameiVSP1_BOTJR
    AccessioniPrimary (citable) accession number: Q2PQJ3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 24, 2007
    Last sequence update: January 24, 2006
    Last modified: October 1, 2014
    This is version 38 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programAnimal Toxin Annotation Program
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Does not show hemorrhagic and myotoxic activities (PubMed:17466550), as well as activity on platelet aggregation and plasmin (represented by the chromogenic substrate S-2251) (PubMed:17996740).2 Publications

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3