Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q2PQJ3

- VSP1_BOTJR

UniProt

Q2PQJ3 - VSP1_BOTJR

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Thrombin-like enzyme BjussuSP-1

Gene
N/A
Organism
Bothrops jararacussu (Jararacussu)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Thrombin-like enzyme that shows clotting activity upon human plasma. Shows specific fibrinogenolytic activity for Aalpha chain (FGA). Hydrolyzes fibrin, BAPNA and TAME, as well as chromogenic artificial substrates of the blood coagulation cascasde: S-27654 for factor X (F10), S-2302 for kallikrein (KLK), factor XIa (F11), and XIIa (F12), and S-2266 for kallikrein and factor XIa (F11). Subcutaneous injection into mice induces a mild edema. Intravenous and intramuscular injection reduce plasma fibrinogen concentration and increase the levels of fibrin(ogen) degradation products. Intramuscular injection also promotes an increase in the expression of proMMP-9, but is unable to activate it.3 Publications

Enzyme regulationi

Inhibited by leupeptin, heparin, and 1.10-phenantroline.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei40 – 401Charge relay systemBy similarity
Active sitei85 – 851Charge relay systemBy similarity
Active sitei178 – 1781Charge relay systemBy similarity

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Blood coagulation cascade activating toxin, Fibrinolytic toxin, Hemostasis impairing toxin, Hydrolase, Protease, Serine protease, Toxin

Keywords - Ligandi

Sialic acid

Protein family/group databases

MEROPSiS01.023.

Names & Taxonomyi

Protein namesi
Recommended name:
Thrombin-like enzyme BjussuSP-1 (EC:3.4.21.-)
Short name:
SVTLE BjussuSP-1
Alternative name(s):
Fibrinogen-clotting enzyme
Jararacussin-I
Snake venom serine protease 1
Short name:
SVSP
Thrombin-like enzyme BjussuSP-I
Short name:
SVTLE BjussuSP-I
OrganismiBothrops jararacussu (Jararacussu)
Taxonomic identifieri8726 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeCrotalinaeBothrops

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 232232Thrombin-like enzyme BjussuSP-1PRO_0000296362Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi7 ↔ 1381 PublicationPROSITE-ProRule annotation
Disulfide bondi25 ↔ 411 PublicationPROSITE-ProRule annotation
Disulfide bondi73 ↔ 2301 PublicationPROSITE-ProRule annotation
Glycosylationi77 – 771N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi117 ↔ 1841 PublicationPROSITE-ProRule annotation
Glycosylationi129 – 1291N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi149 ↔ 1631 PublicationPROSITE-ProRule annotation
Disulfide bondi174 ↔ 1991 PublicationPROSITE-ProRule annotation

Post-translational modificationi

N-glycosylated. Contains sialic acid residues. Deglycosylation reduces in 50% the formation of fibrin clot.2 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

Expressed by the venom gland.

Interactioni

Subunit structurei

Monomer.1 Publication

Structurei

Secondary structure

1
232
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni9 – 113
Beta strandi15 – 2915
Beta strandi31 – 377
Helixi39 – 413
Beta strandi47 – 515
Beta strandi63 – 653
Beta strandi67 – 726
Beta strandi87 – 937
Beta strandi116 – 1238
Beta strandi126 – 1294
Beta strandi137 – 1437
Helixi147 – 1504
Beta strandi161 – 1655
Beta strandi181 – 1844
Beta strandi187 – 1948
Beta strandi206 – 2105
Helixi211 – 2144
Helixi215 – 2239

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4GSOX-ray2.60A1-232[»]
ProteinModelPortaliQ2PQJ3.
SMRiQ2PQJ3. Positions 1-232.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 223223Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family. Snake venom subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Phylogenomic databases

HOVERGENiHBG013304.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2PQJ3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
VLGGDECDIN EHPFLAFLYS HGYFCGLTLI NQEWVVTAAH CDSTNFQMQL
60 70 80 90 100
GVHSKKVLNE DEQTRNPKEK FICPNKNMSE VLDKDIMLIK LDKPISNSKH
110 120 130 140 150
IAPLSLPSNP PSVGSVCRIM GWGSITIPNE TYPDVPYCAN INLVDYEVCQ
160 170 180 190 200
GAYNGLPAKT TLCAGVLEGG KDTCVGDSGG PLICNGQFQG IVSYGAHSCG
210 220 230
QGPKPGIYTN VFDYTDWIQR NIAGNTDATC PP
Length:232
Mass (Da):25,163
Last modified:January 24, 2006 - v1
Checksum:i14C6B2DE6A97064D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ307451 mRNA. Translation: ABC24687.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ307451 mRNA. Translation: ABC24687.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4GSO X-ray 2.60 A 1-232 [» ]
ProteinModelPortali Q2PQJ3.
SMRi Q2PQJ3. Positions 1-232.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi S01.023.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

HOVERGENi HBG013304.

Family and domain databases

InterProi IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00089. Trypsin. 1 hit.
[Graphical view ]
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular characterization of BjussuSP-I, a new thrombin-like enzyme with procoagulant and kallikrein-like activity isolated from Bothrops jararacussu snake venom."
    Sant'Ana C.D., Bernardes C.P., Izidoro L.F., Mazzi M.V., Soares S.G., Fuly A.L., Zingali R.B., Magro A.J., Braz A.S., Fontes M.R., Stabeli R.G., Sampaio S.V., Soares A.M.
    Biochimie 90:500-507(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME REGULATION, GLYCOSYLATION.
    Tissue: Venom gland.
  2. Cited for: PROTEIN SEQUENCE OF 1-20, FUNCTION, SUBUNIT, ENZYME REGULATION, GLYCOSYLATION, SIALIC ACID.
    Tissue: Venom.
  3. "Local and systemic pathophysiological alterations induced by a serine proteinase from the venom of the snake Bothrops jararacussu."
    Perez A.V., Saravia P., Rucavado A., Sant'Ana C.D., Soares A.M., Gutierrez J.M.
    Toxicon 49:1063-1069(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Tissue: Venom.
  4. "Crystal structure of jararacussin-I: the highly negatively charged catalytic interface contributes to macromolecular selectivity in snake venom thrombin-like enzymes."
    Ullah A., Souza T.A., Zanphorlin L.M., Mariutti R.B., Santana V.S., Murakami M.T., Arni R.K.
    Protein Sci. 22:128-132(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS), DISULFIDE BONDS.
    Tissue: Venom.

Entry informationi

Entry nameiVSP1_BOTJR
AccessioniPrimary (citable) accession number: Q2PQJ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: January 24, 2006
Last modified: October 29, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Miscellaneous

Does not show hemorrhagic and myotoxic activities (PubMed:17466550), as well as activity on platelet aggregation and plasmin (represented by the chromogenic substrate S-2251) (PubMed:17996740).2 Publications

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3