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Q2PQJ3 (VSP1_BOTJR) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thrombin-like enzyme BjussuSP-1

Short name=SVTLE BjussuSP-1
EC=3.4.21.-
Alternative name(s):
Fibrinogen-clotting enzyme
Jararacussin-I
Snake venom serine protease 1
Short name=SVSP
Thrombin-like enzyme BjussuSP-I
Short name=SVTLE BjussuSP-I
OrganismBothrops jararacussu (Jararacussu)
Taxonomic identifier8726 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeCrotalinaeBothrops

Protein attributes

Sequence length232 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Thrombin-like enzyme that shows clotting activity upon human plasma. Shows specific fibrinogenolytic activity for Aalpha chain (FGA). Hydrolyzes fibrin, BAPNA and TAME, as well as chromogenic artificial substrates of the blood coagulation cascasde: S-27654 for factor X (F10), S-2302 for kallikrein (KLK), factor XIa (F11), and XIIa (F12), and S-2266 for kallikrein and factor XIa (F11). Subcutaneous injection into mice induces a mild edema. Intravenous and intramuscular injection reduce plasma fibrinogen concentration and increase the levels of fibrin(ogen) degradation products. Intramuscular injection also promotes an increase in the expression of proMMP-9, but is unable to activate it. Ref.1 Ref.2 Ref.3

Enzyme regulation

Inhibited by leupeptin, heparin, and 1.10-phenantroline. Ref.1 Ref.2

Subunit structure

Monomer. Ref.2

Subcellular location

Secreted.

Tissue specificity

Expressed by the venom gland.

Post-translational modification

N-glycosylated. Contains sialic acid residues. Deglycosylation reduces in 50% the formation of fibrin clot. Ref.1 Ref.2

Miscellaneous

Does not show hemorrhagic and myotoxic activities (Ref.2), as well as activity on platelet aggregation and plasmin (represented by the chromogenic substrate S-2251) (Ref.1).

Sequence similarities

Belongs to the peptidase S1 family. Snake venom subfamily.

Contains 1 peptidase S1 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 232232Thrombin-like enzyme BjussuSP-1
PRO_0000296362

Regions

Domain1 – 223223Peptidase S1

Sites

Active site401Charge relay system By similarity
Active site851Charge relay system By similarity
Active site1781Charge relay system By similarity

Amino acid modifications

Glycosylation771N-linked (GlcNAc...) Potential
Glycosylation1291N-linked (GlcNAc...) Potential
Disulfide bond7 ↔ 138 Ref.4
Disulfide bond25 ↔ 41 Ref.4
Disulfide bond73 ↔ 230 Ref.4
Disulfide bond117 ↔ 184 Ref.4
Disulfide bond149 ↔ 163 Ref.4
Disulfide bond174 ↔ 199 Ref.4

Secondary structure

................................... 232
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q2PQJ3 [UniParc].

Last modified January 24, 2006. Version 1.
Checksum: 14C6B2DE6A97064D

FASTA23225,163
        10         20         30         40         50         60 
VLGGDECDIN EHPFLAFLYS HGYFCGLTLI NQEWVVTAAH CDSTNFQMQL GVHSKKVLNE 

        70         80         90        100        110        120 
DEQTRNPKEK FICPNKNMSE VLDKDIMLIK LDKPISNSKH IAPLSLPSNP PSVGSVCRIM 

       130        140        150        160        170        180 
GWGSITIPNE TYPDVPYCAN INLVDYEVCQ GAYNGLPAKT TLCAGVLEGG KDTCVGDSGG 

       190        200        210        220        230 
PLICNGQFQG IVSYGAHSCG QGPKPGIYTN VFDYTDWIQR NIAGNTDATC PP 

« Hide

References

[1]"Molecular characterization of BjussuSP-I, a new thrombin-like enzyme with procoagulant and kallikrein-like activity isolated from Bothrops jararacussu snake venom."
Sant'Ana C.D., Bernardes C.P., Izidoro L.F., Mazzi M.V., Soares S.G., Fuly A.L., Zingali R.B., Magro A.J., Braz A.S., Fontes M.R., Stabeli R.G., Sampaio S.V., Soares A.M.
Biochimie 90:500-507(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME REGULATION, GLYCOSYLATION.
Tissue: Venom gland.
[2]"BjussuSP-I: a new thrombin-like enzyme isolated from Bothrops jararacussu snake venom."
Sant'Ana C.D., Ticli F.K., Oliveira L.L., Giglio J.R., Rechia C.G., Fuly A.L., Selistre de Araujo H.S., Franco J.J., Stabeli R.G., Soares A.M., Sampaio S.V.
Comp. Biochem. Physiol. 151:443-454(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-20, FUNCTION, SUBUNIT, ENZYME REGULATION, GLYCOSYLATION, SIALIC ACID.
Tissue: Venom.
[3]"Local and systemic pathophysiological alterations induced by a serine proteinase from the venom of the snake Bothrops jararacussu."
Perez A.V., Saravia P., Rucavado A., Sant'Ana C.D., Soares A.M., Gutierrez J.M.
Toxicon 49:1063-1069(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
Tissue: Venom.
[4]"Crystal structure of jararacussin-I: the highly negatively charged catalytic interface contributes to macromolecular selectivity in snake venom thrombin-like enzymes."
Ullah A., Souza T.A., Zanphorlin L.M., Mariutti R.B., Santana V.S., Murakami M.T., Arni R.K.
Protein Sci. 22:128-132(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS), DISULFIDE BONDS.
Tissue: Venom.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DQ307451 mRNA. Translation: ABC24687.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4GSOX-ray2.60A1-232[»]
ProteinModelPortalQ2PQJ3.
SMRQ2PQJ3. Positions 1-232.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSS01.023.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

HOVERGENHBG013304.

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameVSP1_BOTJR
AccessionPrimary (citable) accession number: Q2PQJ3
Entry history
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: January 24, 2006
Last modified: June 11, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
Annotation programAnimal Toxin Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references