Q2PQJ3 (VSP1_BOTJR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Thrombin-like enzyme BjussuSP-1 Short name=SVTLE BjussuSP-1 EC=3.4.21.- Alternative name(s): Fibrinogen-clotting enzyme Snake venom serine protease 1 Short name=SVSP Thrombin-like enzyme BjussuSP-I Short name=SVTLE BjussuSP-I
Organism	Bothrops jararacussu (Jararacussu)
Taxonomic identifier	8726 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Sauropsida › Sauria › Lepidosauria › Squamata › Scleroglossa › Serpentes › Colubroidea › Viperidae › Crotalinae › Bothrops

Protein attributes

Sequence length	232 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Thrombin-like enzyme that shows clotting activity upon human plasma. Shows specific fibrinogenolytic activity for Aalpha chain (FGA). Hydrolyzes fibrin, BAPNA and TAME, as well as chromogenic artificial substrates of the blood coagulation cascasde: S-27654 for factor X (F10), S-2302 for kallikrein (KLK), factor XIa (F11), and XIIa (F12), and S-2266 for kallikrein and factor XIa (F11). Subcutaneous injection into mice induces a mild edema. Intravenous and intramuscular injection reduce plasma fibrinogen concentration and increase the levels of fibrin(ogen) degradation products. Intramuscular injection also promotes an increase in the expression of proMMP-9, but is unable to activate it. Ref.1 Ref.2 Ref.3
Enzyme regulation	Inhibited by PMSF, leupeptin, heparin, and 1.10-phenantroline. Ref.1 Ref.2
Subunit structure	Monomer. Ref.2
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Post-translational modification	N-glycosylated. Contains sialic acid residues. Deglycosylation reduces in 50% the formation of fibrin clot. Ref.1 Ref.2
Miscellaneous	Does not show hemorrhagic and myotoxic activities (Ref.2), as well as activity on platelet aggregation and plasmin (represented by the chromogenic substrate S-2251) (Ref.1).
Sequence similarities	Belongs to the peptidase S1 family. Snake venom subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Inflammatory response
Cellular component	Secreted
Ligand	Sialic acid
Molecular function	Blood coagulation cascade activating toxin Fibrinolytic toxin Hemostasis impairing toxin Hydrolase Protease Serine protease Toxin
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological_process	inflammatory response Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 232

232

Thrombin-like enzyme BjussuSP-1

PRO_0000296362

Regions

Domain

1 – 223

223

Peptidase S1

Sites

Active site

40

1

Charge relay system By similarity

Active site

85

1

Charge relay system By similarity

Active site

178

1

Charge relay system By similarity

Amino acid modifications

Glycosylation

77

1

N-linked (GlcNAc...) Potential

Glycosylation

129

1

N-linked (GlcNAc...) Potential

Disulfide bond

7 ↔ 138

By similarity

Disulfide bond

25 ↔ 41

By similarity

Disulfide bond

73 ↔ 230

By similarity

Disulfide bond

117 ↔ 184

By similarity

Disulfide bond

149 ↔ 163

By similarity

Disulfide bond

174 ↔ 199

By similarity

Secondary structure

1

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232

Helix Strand Turn

Details...

Turn

9 – 11

3

Beta strand

15 – 29

15

Beta strand

31 – 37

7

Helix

39 – 41

3

Beta strand

47 – 51

5

Beta strand

63 – 65

3

Beta strand

67 – 72

6

Beta strand

87 – 93

7

Beta strand

116 – 123

8

Beta strand

126 – 129

4

Beta strand

137 – 143

7

Helix

147 – 150

4

Beta strand

161 – 165

5

Beta strand

181 – 184

4

Beta strand

187 – 194

8

Beta strand

206 – 210

5

Helix

211 – 214

4

Helix

215 – 223

9

Sequences

	Sequence		Length	Mass (Da)
	Q2PQJ3 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: 14C6B2DE6A97064D	FASTA	232	25,163
	10 20 30 40 50 60 VLGGDECDIN EHPFLAFLYS HGYFCGLTLI NQEWVVTAAH CDSTNFQMQL GVHSKKVLNE 70 80 90 100 110 120 DEQTRNPKEK FICPNKNMSE VLDKDIMLIK LDKPISNSKH IAPLSLPSNP PSVGSVCRIM 130 140 150 160 170 180 GWGSITIPNE TYPDVPYCAN INLVDYEVCQ GAYNGLPAKT TLCAGVLEGG KDTCVGDSGG 190 200 210 220 230 PLICNGQFQG IVSYGAHSCG QGPKPGIYTN VFDYTDWIQR NIAGNTDATC PP

References

[1]	"Molecular characterization of BjussuSP-I, a new thrombin-like enzyme with procoagulant and kallikrein-like activity isolated from Bothrops jararacussu snake venom." Sant'Ana C.D., Bernardes C.P., Izidoro L.F., Mazzi M.V., Soares S.G., Fuly A.L., Zingali R.B., Magro A.J., Braz A.S., Fontes M.R., Stabeli R.G., Sampaio S.V., Soares A.M. Biochimie 90:500-507(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME REGULATION, GLYCOSYLATION. Tissue: Venom gland.
[2]	"BjussuSP-I: a new thrombin-like enzyme isolated from Bothrops jararacussu snake venom." Sant' Ana C.D., Ticli F.K., Oliveira L.L., Giglio J.R., Rechia C.G., Fuly A.L., Selistre de Araujo H.S., Franco J.J., Stabeli R.G., Soares A.M., Sampaio S.V. Comp. Biochem. Physiol. 151:443-454(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-20, FUNCTION, SUBUNIT, ENZYME REGULATION, GLYCOSYLATION. Tissue: Venom.
[3]	"Local and systemic pathophysiological alterations induced by a serine proteinase from the venom of the snake Bothrops jararacussu." Perez A.V., Saravia P., Rucavado A., Sant'Ana C.D., Soares A.M., Gutierrez J.M. Toxicon 49:1063-1069(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION. Tissue: Venom.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

DQ307451 mRNA. Translation: ABC24687.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4GSO	X-ray	2.60	A	1-232	[»]

ProteinModelPortal

Q2PQJ3.

SMR

Q2PQJ3. Positions 1-232.

ModBase

Search...

Protein family/group databases

MEROPS

S01.023.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Phylogenomic databases

HOVERGEN

HBG013304.

Family and domain databases

InterPro

IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]

Pfam

PF00089. Trypsin. 1 hit.
[Graphical view]

PRINTS

PR00722. CHYMOTRYPSIN.

SMART

SM00020. Tryp_SPc. 1 hit.
[Graphical view]

SUPFAM

SSF50494. Pept_Ser_Cys. 1 hit.

PROSITE

PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

VSP1_BOTJR

Accession

Primary (citable) accession number: Q2PQJ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 24, 2007
Last sequence update:	January 24, 2006
Last modified:	April 3, 2013
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Animal Toxin Annotation Program

Annotation program

Chordata Protein Annotation Program