Reviewed, UniProtKB/Swiss-Prot Q2PQH8 (GDE_CANFA)

Names and origin

Protein names

Recommended name:
    Glycogen debranching enzyme
Alternative name(s):
    Glycogen debrancher
Including the following 2 domains:
    1- Recommended name:
            4-alpha-glucanotransferase
              EC=2.4.1.25
        Alternative name(s):
            Oligo-1,4-1,4-glucantransferase
    2- Recommended name:
            Amylo-alpha-1,6-glucosidase
                Short name=Amylo-1,6-glucosidase
              EC=3.2.1.33
        Alternative name(s):
            Dextrin 6-alpha-D-glucosidase

Gene names

Name:

AGL

Organism

Canis familiaris (Dog)

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis

Protein attributes

Sequence length	1533 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Multifunctional enzyme acting as 1,4-alpha-D-glucan:1,4-alpha-D-glucan 4-alpha-D-glycosyltransferase and amylo-1,6-glucosidase in glycogen degradation By similarity.
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1->4)-alpha-D-glucan. Hydrolysis of (1->6)-alpha-D-glucosidic branch linkages in glycogen phosphorylase limit dextrin.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the glycogen debranching enzyme family.

Ontologies

Keywords
Biological process	Glycogen biosynthesis
Disease	Glycogen storage disease
Molecular function	Glycosidase Glycosyltransferase Hydrolase Transferase
PTM	Phosphoprotein
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4-alpha-glucanotransferase activity Inferred from electronic annotation. Source: EC amylo-alpha-1,6-glucosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1533	1533	Glycogen debranching enzyme		PRO_0000232710
Regions
	Region	1 – ?		4-alpha-glucanotransferase
	Region	? – 1533		Amylo-1,6-glucosidase
Sites
	Active site	527	1	By similarity
	Active site	530	1	By similarity
	Active site	628	1	By similarity
Amino acid modifications
	Modified residue	585	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2PQH8-1 [UniParc]. Last modified January 24, 2006. Version 1. Checksum: 8A6E8A11F2252087	FASTA	1,533	174,828
	10 20 30 40 50 60 MGHSKQIRIL LLNEMEKLEK TLFRLEQGFE LQFRLGPTLQ GKAVTVYTNY PFPGETFNRE 70 80 90 100 110 120 KFRSLEWENP TEREDDSDKY CKLNLQQAGS FQYYFLQGNE KSGGGYIVVD PILYVGADNH 130 140 150 160 170 180 VLPLDCVTLQ TFLAKCLGPF DEWESRLRVA KESGYNMIHF TPLQTLGLSR SCYSLANQLE 190 200 210 220 230 240 LNPDFSRPNK KYTWSDVGQL VEKMKKEWNV LCITDVVYNH TAANSKWIQE HPESAYNLVN 250 260 270 280 290 300 SPHLKPAWVL DRALWHLSCD VAEGKYKEKG VPALIENDHQ MNCIRKIIWE DIFPKIQLWE 310 320 330 340 350 360 FFQVDVYKAV EQFRRLLTQE NRKITTKPDP KEHLKIIQDP EYRRLGCTVD MNIALATFIP 370 380 390 400 410 420 HDKGPAAIDE CCNWFRKRIE ELNSEKHQLV NYHQEQAVNC LLGNVFYERM AGHGPKLGPV 430 440 450 460 470 480 TRKHPLVTRY FTFPFEEMTV STEESMIHNP NKACFLMAHN GWVMGDDPLR NFAEPGSEVY 490 500 510 520 530 540 LRRELICWGD SVKLRYGNKP EDCPYLWAHM KKYTEITATY FQGVRLDNCH STPLHVAEYM 550 560 570 580 590 600 LDAARKLQPN LYVVAELFTG SEDLDNIFVT RLGISSLIRE AMSAYNSHEE GRLVYRYGGE 610 620 630 640 650 660 PVGSFVQPCL RPLMPAIAHA LFMDITHDNE CPIVHRSEYD ALPSTTIVSM ACCASGSTKG 670 680 690 700 710 720 YDELVPHQIS VVSEERFYTK WNPGASPSNT GEVNFQSGII AARCAINKLH QELGAQGFIQ 730 740 750 760 770 780 VYVDQVDEDI VAVTRHSPSI HQSVVSVSRT AFRNPKTSFY SKEVPQMCIP GKIEEVVLEA 790 800 810 820 830 840 RTIERNTKPY QKDKNSINGM PNITVEIREH IQLSESKIVK QAGVATKGPN EYIQEIEFEN 850 860 870 880 890 900 LSPGSVIIFR VSLDPHAQVA VGILRNHLTQ FSPHFKSGSL AVENSDPILK IPFAFIASKL 910 920 930 940 950 960 TLAELNQVLY RCEAEEQEDG GGCYDIPNWS SLKYAGLQGL MSVLAEIRPK NDLGHPFCDN 970 980 990 1000 1010 1020 LRSGDWMIDY VSNRLISRSG TIAEVGKWFQ AMFFYLKQIP RYLIPCYFDA ILIGAYTTLL 1030 1040 1050 1060 1070 1080 DIAWKQMSSF VQNGSTFVKH LSLGSVQMCG VGKCPSLPLL SPSLMDVPYR LNEITKEKEQ 1090 1100 1110 1120 1130 1140 CCVSLAAGLP HFSSGIFRCW GRDTFIALRG LLLITGRYLE ARNIILAFAG TLRHGLIPNL 1150 1160 1170 1180 1190 1200 LGEGTYARYN CRDAVWWWLQ CIQDYCKMVP NGLDILKCPV SRMYPTDDSV PLSAGTLDQP 1210 1220 1230 1240 1250 1260 LFEVIQEVMQ RHIQGIQFRE RNAGPQIDRN MKDEGFNITA GVDEETGFVY GGNRLNCGTW 1270 1280 1290 1300 1310 1320 MDKMGESDRA RNRGIPATPR DGSAVEIVGL SKSTVRWLLE LSKKRIFPYH EVRVKRHGKV 1330 1340 1350 1360 1370 1380 VTISYDEWNK KIQDNFEKLF HVSEDPXDFN EKHPNLVHKR GIYKDSYGAS SPWCDYQLRP 1390 1400 1410 1420 1430 1440 NFTIAMVVAP ELFTAEKAWK ALEIAEKKLL GPLGMKTLDP DDMVYCGIYD NALDNDNYNL 1450 1460 1470 1480 1490 1500 AKGFNYHQGP EWLWPVGYFL RAKLYFSKLM GPEANAKTVF LVKNILSRHY VHLERSPWKG 1510 1520 1530 LPELTNENGQ YCPFSCETQA WSIATVLETL YDL

References

[1]	"Glycogen storage disease type IIIa in curly-coated retrievers." Gregory B.L., Shelton G.D., Bali D.S., Chen Y.T., Fyfe J.C. J. Vet. Intern. Med. 21:40-46(2007) [PubMed: 17338148] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	DQ307574 mRNA. Translation: ABC25005.1.
RefSeq	NP_001041561.1.
UniGene	Cfa.18682
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH13. Glycoside Hydrolase Family 13.
Genome annotation databases
Ensembl	ENSCAFG00000020040. Canis familiaris. [Contig view]
GeneID	479931.
KEGG	cfa:479931.
Phylogenomic databases
HOVERGEN	Q2PQH8.
Enzyme and pathway databases
BRENDA	2.4.1.25. 463. 3.2.1.33. 463.
Family and domain databases
InterPro	IPR010401. GDE_C. IPR006421. Glyc_debranch. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR10569. GDE_C. 1 hit.
Pfam	PF06202. GDE_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR01531. glyc_debranch. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GDE_CANFA

Accession

Primary (citable) accession number: Q2PQH8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 18, 2006
Last sequence update:	January 24, 2006
Last modified:	June 16, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)