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Protein

Kinesin-1 heavy chain

Gene

Kif5b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner. Regulates centrosome and nuclear positioning during mitotic entry. During the G2 phase of the cell cycle in a BICD2-dependent manner, antagonizes dynein function and drives the separation of nuclei and centrosomes.HMP:

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi85 – 92ATPIEP:8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent microtubule motor activity, plus-end-directed Source: GO_Central
  • cadherin binding Source: RGD
  • identical protein binding Source: Ensembl
  • JUN kinase binding Source: RGD
  • microtubule binding Source: HGNC
  • microtubule lateral binding Source: CAFA
  • microtubule motor activity Source: HGNC

GO - Biological processi

  • axon guidance Source: GO_Central
  • brain development Source: RGD
  • centrosome localization Source: UniProtKB
  • cytoplasm organization Source: RGD
  • hippocampus development Source: RGD
  • plus-end-directed vesicle transport along microtubule Source: RGD
  • positive regulation of insulin secretion involved in cellular response to glucose stimulus Source: RGD
  • positive regulation of intracellular protein transport Source: RGD
  • positive regulation of potassium ion transport Source: RGD
  • positive regulation of protein localization to plasma membrane Source: RGD
  • positive regulation of synaptic transmission, GABAergic Source: RGD
  • positive regulation of vesicle fusion Source: RGD
  • positive regulation of voltage-gated sodium channel activity Source: RGD
  • regulation of membrane potential Source: RGD
  • stress granule disassembly Source: RGD
  • vesicle transport along microtubule Source: HGNC

Keywordsi

Molecular functionMotor protein
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Kinesin-1 heavy chain
Alternative name(s):
Conventional kinesin heavy chain
Ubiquitous kinesin heavy chain
Short name:
UKHC
Gene namesi
Name:Kif5bTAS:
Synonyms:KhcIBD:
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 17

Organism-specific databases

RGDi621559. Kif5b.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedHMP:
ChainiPRO_00002444852 – 963Kinesin-1 heavy chainAdd BLAST962

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineHMP:1
Cross-linki213Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)HMP:
Modified residuei933PhosphoserineND:1
Modified residuei945PhosphoserineHMP:1
Modified residuei956Omega-N-methylarginineHMP:1

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ2PQA9.
PRIDEiQ2PQA9.

PTM databases

iPTMnetiQ2PQA9.
PhosphoSitePlusiQ2PQA9.

Expressioni

Tissue specificityi

Found in newborn and adult brain, liver, kidney, spleen, heart, lung and sciatic nerve.1 Publication

Gene expression databases

BgeeiENSRNOG00000017466.
GenevisibleiQ2PQA9. RN.

Interactioni

Subunit structurei

Oligomer composed of two heavy chains and two light chains. Interacts with GRIP1, JAKMIP1, PLEKHM2 and SYBU. Interacts with ECM29 and PPP1R42. Interacts with ZFYVE27.HMP:

Binary interactionsi

WithEntry#Exp.IntActNotes
TRAK1Q9UPV93EBI-920191,EBI-1105048From Homo sapiens.

GO - Molecular functioni

  • cadherin binding Source: RGD
  • identical protein binding Source: Ensembl
  • JUN kinase binding Source: RGD
  • microtubule binding Source: HGNC
  • microtubule lateral binding Source: CAFA

Protein-protein interaction databases

BioGridi250763. 1 interactor.
CORUMiQ2PQA9.
DIPiDIP-37054N.
IntActiQ2PQA9. 9 interactors.
MINTiMINT-3391010.
STRINGi10116.ENSRNOP00000023860.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4ATXelectron microscopy8.20C1-340[»]
ProteinModelPortaliQ2PQA9.
SMRiQ2PQA9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 325Kinesin motorIEP:Add BLAST318

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni915 – 963GlobularIEP:Add BLAST49

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili330 – 913IEP:Add BLAST584

Domaini

Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.IKR:

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0240. Eukaryota.
COG5059. LUCA.
GeneTreeiENSGT00890000139323.
HOGENOMiHOG000216718.
HOVERGENiHBG006210.
InParanoidiQ2PQA9.
KOiK10396.
OMAiNDCAKKI.
OrthoDBiEOG091G02A2.
PhylomeDBiQ2PQA9.
TreeFamiTF105225.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiView protein in InterPro
IPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR036961. Kinesin_motor_dom_sf.
IPR027417. P-loop_NTPase.
PANTHERiPTHR24115. PTHR24115. 1 hit.
PfamiView protein in Pfam
PF00225. Kinesin. 1 hit.
PRINTSiPR00380. KINESINHEAVY.
SMARTiView protein in SMART
SM00129. KISc. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q2PQA9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADPAECNIK VMCRFRPLNE SEVNRGDKYV AKFQGEDTVM IASKPYAFDR
60 70 80 90 100
VFQSSTSQEQ VYNDCAKKIV KDVLEGYNGT IFAYGQTSSG KTHTMEGKLH
110 120 130 140 150
DPEGMGIIPR IVQDIFNYIY SMDENLEFHI KVSYFEIYLD KIRDLLDVSK
160 170 180 190 200
TNLSVHEDKN RVPYVKGCTE RFVCSPDEVM DTIDEGKSNR HVAVTNMNEH
210 220 230 240 250
SSRSHSIFLI NVKQENTQTE QKLSGKLYLV DLAGSEKVSK TGAEGAVLDE
260 270 280 290 300
AKNINKSLSA LGNVISALAE GSTYVPYRDS KMTRILQDSL GGNCRTTIVI
310 320 330 340 350
CCSPSSYNES ETKSTLLFGQ RAKTIKNTVC VNVELTAEQW KKKYEKEKEK
360 370 380 390 400
NKTLRNTIQW LENELNRWRN GETVPIDEQF DKEKANLEAF TADKDVAITN
410 420 430 440 450
DKPAAAIGMA GSFTDAERRK CEEEIAKLYK QLDDKDEEIN QQSQLVEKLK
460 470 480 490 500
TQMLDQEELL ASTRRDQDNM QAELNRLQAE NDASKEEVKE VLQALEELAV
510 520 530 540 550
NYDQKSQEVE DKTKEYELLS DELNQKSATL ASIDAELQKL KEMTNHQKKR
560 570 580 590 600
AAEMMASLLK DLAEIGIAVG NNDVKQPEGT GMIDEEFTVA RLYISKMKSE
610 620 630 640 650
VKTMVKRCKQ LESTQTESNK KMEENEKELA ACQLRISQHE AKIKSLTEYL
660 670 680 690 700
QNVEQKKRQL EESVDSLGEE LVQLRAQEKV HEMEKEHLNK VQTANEVKQA
710 720 730 740 750
VEQQIQSHRE THQKQISSLR DEVEAKEKLI TDLQDQNQKM VLEQERLRVE
760 770 780 790 800
HERLKAVDQE KSRKLHELTV MQDRREQARQ DLKGLEETVA KELQTLHNLR
810 820 830 840 850
KLFVQDLATR VKKSAEVDSD DTGGSAAQKQ KISFLENNLE QLTKVHKQLV
860 870 880 890 900
RDNADLRCEL PKLEKRLRAT AERVKALESA LKEAKENASR DRKRYQQEVD
910 920 930 940 950
RIKEAVRSKN MARRGHSAQI AKPIRPGQHP AASPTHPGAV RGGGSFVQNN
960
QPVGLRGGGG KQA
Length:963
Mass (Da):109,531
Last modified:January 24, 2006 - v1
Checksum:i0A460B2CBA47EC30
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ309275 mRNA. Translation: ABC25059.1.
AF155822 mRNA. Translation: AAD39239.1.
RefSeqiNP_476550.1. NM_057202.1.
XP_006254067.1. XM_006254005.1.
UniGeneiRn.219283.
Rn.4046.

Genome annotation databases

EnsembliENSRNOT00000023861; ENSRNOP00000023860; ENSRNOG00000017466.
GeneIDi117550.
KEGGirno:117550.
UCSCiRGD:621559. rat.

Similar proteinsi

Entry informationi

Entry nameiKINH_RAT
AccessioniPrimary (citable) accession number: Q2PQA9
Secondary accession number(s): Q9WV65
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: January 24, 2006
Last modified: November 22, 2017
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families