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Protein

Kinesin-1 heavy chain

Gene

Kif5b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi85 – 92ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent microtubule motor activity, plus-end-directed Source: GO_Central
  • JUN kinase binding Source: RGD
  • microtubule binding Source: HGNC
  • microtubule motor activity Source: HGNC

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Kinesin-1 heavy chain
Alternative name(s):
Conventional kinesin heavy chain
Ubiquitous kinesin heavy chain
Short name:
UKHC
Gene namesi
Name:Kif5bImported
Synonyms:Khc1 Publication
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 17

Organism-specific databases

RGDi621559. Kif5b.

Subcellular locationi

  • Cytoplasmcytoskeleton 1 Publication

  • Note: Uniformly distributed between soma and neurites in hippocampal neurons.

GO - Cellular componenti

  • axonal growth cone Source: RGD
  • cell-cell adherens junction Source: Ensembl
  • ciliary rootlet Source: Ensembl
  • cytoplasm Source: RGD
  • endocytic vesicle Source: Ensembl
  • kinesin complex Source: GO_Central
  • membrane Source: Ensembl
  • microtubule Source: UniProtKB-KW
  • microtubule organizing center Source: Ensembl
  • perinuclear region of cytoplasm Source: HGNC
  • vesicle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002444852 – 963Kinesin-1 heavy chainAdd BLAST962

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei933PhosphoserineCombined sources1
Modified residuei945PhosphoserineBy similarity1
Modified residuei956Omega-N-methylarginineBy similarity1

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ2PQA9.
PRIDEiQ2PQA9.

PTM databases

iPTMnetiQ2PQA9.
PhosphoSitePlusiQ2PQA9.

Expressioni

Tissue specificityi

Found in newborn and adult brain, liver, kidney, spleen, heart, lung and sciatic nerve.1 Publication

Gene expression databases

BgeeiENSRNOG00000017466.
GenevisibleiQ2PQA9. RN.

Interactioni

Subunit structurei

Oligomer composed of two heavy chains and two light chains. Interacts with GRIP1, JAKMIP1, PLEKHM2 and SYBU. Interacts with ECM29 and PPP1R42. Interacts with ZFYVE27.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
TRAK1Q9UPV93EBI-920191,EBI-1105048From a different organism.

GO - Molecular functioni

  • JUN kinase binding Source: RGD
  • microtubule binding Source: HGNC

Protein-protein interaction databases

BioGridi250763. 1 interactor.
DIPiDIP-37054N.
IntActiQ2PQA9. 8 interactors.
MINTiMINT-3391010.
STRINGi10116.ENSRNOP00000023860.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4ATXelectron microscopy8.20C1-340[»]
ProteinModelPortaliQ2PQA9.
SMRiQ2PQA9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 325Kinesin motorPROSITE-ProRule annotationAdd BLAST318

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni915 – 963GlobularSequence analysisAdd BLAST49

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili330 – 913Sequence analysisAdd BLAST584

Domaini

Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.Curated

Sequence similaritiesi

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin subfamily.PROSITE-ProRule annotation
Contains 1 kinesin motor domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0240. Eukaryota.
COG5059. LUCA.
GeneTreeiENSGT00860000133684.
HOGENOMiHOG000216718.
HOVERGENiHBG006210.
InParanoidiQ2PQA9.
KOiK10396.
OMAiLAECNIK.
OrthoDBiEOG091G02A2.
PhylomeDBiQ2PQA9.
TreeFamiTF105225.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 1 hit.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q2PQA9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADPAECNIK VMCRFRPLNE SEVNRGDKYV AKFQGEDTVM IASKPYAFDR
60 70 80 90 100
VFQSSTSQEQ VYNDCAKKIV KDVLEGYNGT IFAYGQTSSG KTHTMEGKLH
110 120 130 140 150
DPEGMGIIPR IVQDIFNYIY SMDENLEFHI KVSYFEIYLD KIRDLLDVSK
160 170 180 190 200
TNLSVHEDKN RVPYVKGCTE RFVCSPDEVM DTIDEGKSNR HVAVTNMNEH
210 220 230 240 250
SSRSHSIFLI NVKQENTQTE QKLSGKLYLV DLAGSEKVSK TGAEGAVLDE
260 270 280 290 300
AKNINKSLSA LGNVISALAE GSTYVPYRDS KMTRILQDSL GGNCRTTIVI
310 320 330 340 350
CCSPSSYNES ETKSTLLFGQ RAKTIKNTVC VNVELTAEQW KKKYEKEKEK
360 370 380 390 400
NKTLRNTIQW LENELNRWRN GETVPIDEQF DKEKANLEAF TADKDVAITN
410 420 430 440 450
DKPAAAIGMA GSFTDAERRK CEEEIAKLYK QLDDKDEEIN QQSQLVEKLK
460 470 480 490 500
TQMLDQEELL ASTRRDQDNM QAELNRLQAE NDASKEEVKE VLQALEELAV
510 520 530 540 550
NYDQKSQEVE DKTKEYELLS DELNQKSATL ASIDAELQKL KEMTNHQKKR
560 570 580 590 600
AAEMMASLLK DLAEIGIAVG NNDVKQPEGT GMIDEEFTVA RLYISKMKSE
610 620 630 640 650
VKTMVKRCKQ LESTQTESNK KMEENEKELA ACQLRISQHE AKIKSLTEYL
660 670 680 690 700
QNVEQKKRQL EESVDSLGEE LVQLRAQEKV HEMEKEHLNK VQTANEVKQA
710 720 730 740 750
VEQQIQSHRE THQKQISSLR DEVEAKEKLI TDLQDQNQKM VLEQERLRVE
760 770 780 790 800
HERLKAVDQE KSRKLHELTV MQDRREQARQ DLKGLEETVA KELQTLHNLR
810 820 830 840 850
KLFVQDLATR VKKSAEVDSD DTGGSAAQKQ KISFLENNLE QLTKVHKQLV
860 870 880 890 900
RDNADLRCEL PKLEKRLRAT AERVKALESA LKEAKENASR DRKRYQQEVD
910 920 930 940 950
RIKEAVRSKN MARRGHSAQI AKPIRPGQHP AASPTHPGAV RGGGSFVQNN
960
QPVGLRGGGG KQA
Length:963
Mass (Da):109,531
Last modified:January 24, 2006 - v1
Checksum:i0A460B2CBA47EC30
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ309275 mRNA. Translation: ABC25059.1.
AF155822 mRNA. Translation: AAD39239.1.
RefSeqiNP_476550.1. NM_057202.1.
XP_006254067.1. XM_006254005.1.
UniGeneiRn.219283.
Rn.4046.

Genome annotation databases

EnsembliENSRNOT00000023861; ENSRNOP00000023860; ENSRNOG00000017466.
GeneIDi117550.
KEGGirno:117550.
UCSCiRGD:621559. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ309275 mRNA. Translation: ABC25059.1.
AF155822 mRNA. Translation: AAD39239.1.
RefSeqiNP_476550.1. NM_057202.1.
XP_006254067.1. XM_006254005.1.
UniGeneiRn.219283.
Rn.4046.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4ATXelectron microscopy8.20C1-340[»]
ProteinModelPortaliQ2PQA9.
SMRiQ2PQA9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi250763. 1 interactor.
DIPiDIP-37054N.
IntActiQ2PQA9. 8 interactors.
MINTiMINT-3391010.
STRINGi10116.ENSRNOP00000023860.

PTM databases

iPTMnetiQ2PQA9.
PhosphoSitePlusiQ2PQA9.

Proteomic databases

PaxDbiQ2PQA9.
PRIDEiQ2PQA9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000023861; ENSRNOP00000023860; ENSRNOG00000017466.
GeneIDi117550.
KEGGirno:117550.
UCSCiRGD:621559. rat.

Organism-specific databases

CTDi3799.
RGDi621559. Kif5b.

Phylogenomic databases

eggNOGiKOG0240. Eukaryota.
COG5059. LUCA.
GeneTreeiENSGT00860000133684.
HOGENOMiHOG000216718.
HOVERGENiHBG006210.
InParanoidiQ2PQA9.
KOiK10396.
OMAiLAECNIK.
OrthoDBiEOG091G02A2.
PhylomeDBiQ2PQA9.
TreeFamiTF105225.

Miscellaneous databases

PROiQ2PQA9.

Gene expression databases

BgeeiENSRNOG00000017466.
GenevisibleiQ2PQA9. RN.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 1 hit.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKINH_RAT
AccessioniPrimary (citable) accession number: Q2PQA9
Secondary accession number(s): Q9WV65
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: January 24, 2006
Last modified: November 30, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.