Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Serine/threonine-protein kinase sgk-1

Gene

sgk-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts downstream of age-1 and pdk-1 in the daf-2/insulin receptor-like transduction pathway. Essential role in regulating development, stress response, and longevity. Phosphorylates Forkhead-related daf-16 and the longevity-promoting skn-1 transcription factors, which inhibits their entry into the nucleus and antagonizes their function. Acts downstream of rict-1 to regulate fat storage, size, and development. Does not appear to play a role in immune function.5 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Cofactori

Mg2+1 Publication

Enzyme regulationi

Phosphorylated and activated by pdk-1.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei164 – 1641ATPPROSITE-ProRule annotationBy similarity
Active sitei259 – 2591Proton acceptorPROSITE-ProRule annotationBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi141 – 1499ATPPROSITE-ProRule annotationBy similarity

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • phosphatidylinositol-3,4,5-trisphosphate binding Source: WormBase
  • protein kinase activity Source: WormBase
  • protein serine/threonine kinase activity Source: WormBase

GO - Biological processi

  • intracellular signal transduction Source: GO_Central
  • mesendoderm development Source: WormBase
  • nematode larval development Source: WormBase
  • peptidyl-serine phosphorylation Source: WormBase
  • protein phosphorylation Source: WormBase
  • regulation of protein localization to basolateral plasma membrane Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Stress response

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-2672351. Stimuli-sensing channels.
R-CEL-6804757. Regulation of TP53 Degradation.
SignaLinkiQ2PJ68.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase sgk-11 PublicationBy similarity (EC:2.7.11.1)
Alternative name(s):
Serum- and glucocorticoid-inducible kinase homolog1 Publication
Serum/glucocorticoid-regulated kinase 1By similarity
Gene namesi
Name:sgk-1Imported
ORF Names:W10G6.2
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome X

Organism-specific databases

WormBaseiW10G6.2a; CE39527; WBGene00004789; sgk-1.
W10G6.2b; CE39528; WBGene00004789; sgk-1.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleus 1 Publication

  • Note: Localized both in the cytoplasm and in the nucleus in neurons, exclusively cytoplasmic in the intestine.1 Publication

GO - Cellular componenti

  • cytoplasm Source: WormBase
  • nucleus Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

A mild developmental delay, extended generation time, an extension of life span, defective egg-laying and decreased body size, but increased lipid storage.4 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 463463Serine/threonine-protein kinase sgk-1PRO_0000385374Add
BLAST

Proteomic databases

EPDiQ2PJ68.
PaxDbiQ2PJ68.

Expressioni

Tissue specificityi

Expressed in late embryos just before hatching. At postembryonic stages, expressed in sensory and motor neurons and in the intestine.1 Publication

Interactioni

Subunit structurei

Interacts with pdk-1, akt-1, akt-2 and daf-16. Part of a complex containing sgk-1, akt-1 and akt-2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
akt-1Q179413EBI-1770776,EBI-1770718
akt-2Q9XTG73EBI-1770776,EBI-320656
daf-16O168503EBI-1770776,EBI-324028

Protein-protein interaction databases

IntActiQ2PJ68. 6 interactions.
STRINGi6239.W10G6.2b.

Structurei

3D structure databases

ProteinModelPortaliQ2PJ68.
SMRiQ2PJ68. Positions 97-458.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini135 – 392258Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini393 – 46371AGC-kinase C-terminalSequence analysisAdd
BLAST

Sequence similaritiesi

Contains 1 AGC-kinase C-terminal domain.Sequence analysis
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0598. Eukaryota.
ENOG410XNPH. LUCA.
GeneTreeiENSGT00820000126961.
HOGENOMiHOG000233033.
InParanoidiQ2PJ68.
KOiK13302.
OMAiHIVEHNE.
OrthoDBiEOG7Q5HCW.
PhylomeDBiQ2PJ68.

Family and domain databases

InterProiIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform b1 Publication (identifier: Q2PJ68-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGSMYYNESR MVRKDEVTCN VIIGDDKKTV VYALRIGNGP IMQKTFEEYE
60 70 80 90 100
RFFTTEKDMI PATIFTAPKK KFLQADSKFY EKRRVWILVI SQHLVDNNLR
110 120 130 140 150
SEDVRRFFHL ESPDDDENNV DLGPSERKTA TANDFDYLTT IGKGSFGRVY
160 170 180 190 200
QVRHKETKKI YAMKILSKEH IRKKNEVKHV MAERNVLINN FKHPFLVSLH
210 220 230 240 250
FSFQNKEKLY FVLDHLNGGE LFSHLQREKH FSESRSRFYA AEIACALGYL
260 270 280 290 300
HEKNIIYRDL KPENLLLDDK GYLVLTDFGL CKEDMQGSKT TSTFCGTPEY
310 320 330 340 350
LAPEIILKKP YDKTVDWWCL GSVLYEMIFG LPPFYSKDHN EMYDKIINQP
360 370 380 390 400
LRLKHNISVP CSELITGLLQ KDRSKRLGHR NDFRDIRDHP FFLPVDWDKL
410 420 430 440 450
LNRELKAPFI PKVKNAMDTS NISKEFVEIQ IDPSSLAPQQ LAVTHRDHDF
460
ENFTFVDTNR VLV
Note: No experimental confirmation available.Curated
Length:463
Mass (Da):54,291
Last modified:January 24, 2006 - v1
Checksum:iC2006B90F4E9FBCD
GO
Isoform a1 Publication (identifier: Q2PJ68-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-10: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:453
Mass (Da):53,072
Checksum:iA2ACC870B5D57B08
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1010Missing in isoform a. 1 PublicationVSP_053156

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z81140 Genomic DNA. Translation: CAJ55246.1.
Z81140 Genomic DNA. Translation: CAJ55247.1.
RefSeqiNP_001041298.1. NM_001047833.4. [Q2PJ68-2]
NP_001041299.1. NM_001047834.1. [Q2PJ68-1]
UniGeneiCel.19781.

Genome annotation databases

EnsemblMetazoaiW10G6.2b; W10G6.2b; WBGene00004789. [Q2PJ68-1]
GeneIDi181697.
KEGGicel:CELE_W10G6.2.
UCSCiW10G6.2a.1. c. elegans. [Q2PJ68-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z81140 Genomic DNA. Translation: CAJ55246.1.
Z81140 Genomic DNA. Translation: CAJ55247.1.
RefSeqiNP_001041298.1. NM_001047833.4. [Q2PJ68-2]
NP_001041299.1. NM_001047834.1. [Q2PJ68-1]
UniGeneiCel.19781.

3D structure databases

ProteinModelPortaliQ2PJ68.
SMRiQ2PJ68. Positions 97-458.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ2PJ68. 6 interactions.
STRINGi6239.W10G6.2b.

Proteomic databases

EPDiQ2PJ68.
PaxDbiQ2PJ68.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiW10G6.2b; W10G6.2b; WBGene00004789. [Q2PJ68-1]
GeneIDi181697.
KEGGicel:CELE_W10G6.2.
UCSCiW10G6.2a.1. c. elegans. [Q2PJ68-1]

Organism-specific databases

CTDi181697.
WormBaseiW10G6.2a; CE39527; WBGene00004789; sgk-1.
W10G6.2b; CE39528; WBGene00004789; sgk-1.

Phylogenomic databases

eggNOGiKOG0598. Eukaryota.
ENOG410XNPH. LUCA.
GeneTreeiENSGT00820000126961.
HOGENOMiHOG000233033.
InParanoidiQ2PJ68.
KOiK13302.
OMAiHIVEHNE.
OrthoDBiEOG7Q5HCW.
PhylomeDBiQ2PJ68.

Enzyme and pathway databases

ReactomeiR-CEL-2672351. Stimuli-sensing channels.
R-CEL-6804757. Regulation of TP53 Degradation.
SignaLinkiQ2PJ68.

Miscellaneous databases

PROiQ2PJ68.

Family and domain databases

InterProiIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.
  2. "C. elegans SGK-1 is the critical component in the Akt/PKB kinase complex to control stress response and life span."
    Hertweck M., Goebel C., Baumeister R.
    Dev. Cell 6:577-588(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ENZYME REGULATION, INTERACTION WITH PDK-1; AKT-1; AKT-2 AND DAF-16, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
  3. "The DAF-2 insulin-like signaling pathway independently regulates aging and immunity in C. elegans."
    Evans E.A., Chen W.C., Tan M.-W.
    Aging Cell 7:879-893(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  4. "Direct inhibition of the longevity-promoting factor SKN-1 by insulin-like signaling in C. elegans."
    Tullet J.M., Hertweck M., An J.H., Baker J., Hwang J.Y., Liu S., Oliveira R.P., Baumeister R., Blackwell T.K.
    Cell 132:1025-1038(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Rictor/TORC2 regulates fat metabolism, feeding, growth, and life span in Caenorhabditis elegans."
    Soukas A.A., Kane E.A., Carr C.E., Melo J.A., Ruvkun G.
    Genes Dev. 23:496-511(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  6. "Rictor/TORC2 regulates Caenorhabditis elegans fat storage, body size, and development through sgk-1."
    Jones K.T., Greer E.R., Pearce D., Ashrafi K.
    PLoS Biol. 7:60-60(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiSGK1_CAEEL
AccessioniPrimary (citable) accession number: Q2PJ68
Secondary accession number(s): Q2PJ69
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: January 24, 2006
Last modified: July 6, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.