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Protein

Thiol-activated cytolysin

Gene

samhpaf

Organism
Streptococcus mitis
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Sulfhydryl-activated toxin that causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the protein undergoes a major conformation change, leading to its insertion in the host membrane and formation of an oligomeric pore complex.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi68Calcium; via carbonyl oxygenCombined sources1
Metal bindingi73Calcium; via carbonyl oxygenCombined sources1
Metal bindingi82CalciumCombined sources1
Metal bindingi176Calcium; via carbonyl oxygenCombined sources1
Metal bindingi177CalciumCombined sources1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionToxinUniRule annotation
Biological processCytolysis, HemolysisUniRule annotation
LigandCalciumCombined sources, Lipid-bindingUniRule annotation, Metal-bindingCombined sources

Protein family/group databases

CAZyiCBM47. Carbohydrate-Binding Module Family 47.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiol-activated cytolysinUniRule annotation
Gene namesi
Name:samhpafImported
OrganismiStreptococcus mitisImported
Taxonomic identifieri28037 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Subcellular locationi

  • Secreted UniRule annotation
  • Host cell membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Host cell membraneUniRule annotation, Host membrane, Membrane, SecretedUniRule annotation

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 36UniRule annotationAdd BLAST36
ChainiPRO_500973766437 – 665Thiol-activated cytolysinUniRule annotationAdd BLAST629

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3LE0X-ray1.91A38-190[»]
3LEGX-ray2.01A38-190[»]
3LEIX-ray1.90A38-190[»]
3LEKX-ray2.00A38-190[»]
4GWIX-ray1.60A38-190[»]
4GWJX-ray1.60A38-190[»]
ProteinModelPortaliQ2PHL4.
SMRiQ2PHL4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ2PHL4.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 183F5/8 type CInterPro annotationAdd BLAST154

Sequence similaritiesi

Belongs to the thiol-activated cytolysin family.UniRule annotation

Keywords - Domaini

SignalUniRule annotation, Transmembrane, Transmembrane beta strandUniRule annotation

Phylogenomic databases

eggNOGiENOG4105E5P. Bacteria.
ENOG410XQPX. LUCA.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
3.90.840.10. 2 hits.
InterProiView protein in InterPro
IPR000421. FA58C.
IPR006585. FTP1.
IPR008979. Galactose-bd-like.
IPR001869. Thiol_cytolysin.
PfamiView protein in Pfam
PF00754. F5_F8_type_C. 1 hit.
PF01289. Thiol_cytolysin. 1 hit.
PRINTSiPR01400. TACYTOLYSIN.
SMARTiView protein in SMART
SM00607. FTP. 1 hit.
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF56978. SSF56978. 1 hit.
PROSITEiView protein in PROSITE
PS50022. FA58C_3. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2PHL4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNQEKRLHRF VKKCGLGVCS AVVAAFLLNA QGVALATEQG NRPVETENIA
60 70 80 90 100
RGKQASQSST AYGGAAARAV DGNVDSDYGH HSVTHTNFED NAWWQVDLGK
110 120 130 140 150
TENVGKVKLY NRGDGNVANR LSNFDVVLLN EAKQEVARQH FDSLNGKAEL
160 170 180 190 200
EVFFTAKAAR YVKVELKTKN TPLSLAEVEV FRSATTQVGQ DRTAPVVDQT
210 220 230 240 250
SALKDYLFGL AYNPLDILTR KGETLENRYN TSAKEQNGEF VVVEKIKKTL
260 270 280 290 300
STGTADVSIN GNQNVFLGGL YKANQNLLEN QPELISLARA KGTVSVDLPG
310 320 330 340 350
MIQSDSRIEA DPTTSGMQSA MNTLVERWTK NYSSSHSVPA RVQYESTTAY
360 370 380 390 400
SMNQLKAKFG ADFEKAGAPL KIDFEAVQKG EKQIEVVNFK QIYYTATFDA
410 420 430 440 450
PTNPAAVFDK SVTPEDLKQR GVDSQTPPVY VSNVSYGRQI YVKFESASKS
460 470 480 490 500
TELKAAINAV IKGATIAPNS EWSRLLKNTS VTAVIVGGNA SGAAKVVTGT
510 520 530 540 550
VENLKELIRE GANFSAQSPA VPISYKTAFL KDNAQATLQN STDYIETKVT
560 570 580 590 600
SYKNGFLKLH HKGAYVARYY IYWDEITYDE QGNPEIRSRQ WEDNGKNRTS
610 620 630 640 650
GFQTEIQFRG NVRNLRIKVQ EKTGLVWEPW RTVYNRTDLP LVQQRTITHW
660
GTTLNPKVDE KIVNE
Length:665
Mass (Da):73,598
Last modified:February 7, 2006 - v1
Checksum:iC5ED3F30AA91BA96
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB051299 Genomic DNA. Translation: BAE72438.1.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiQ2PHL4_STRMT
AccessioniPrimary (citable) accession number: Q2PHL4
Entry historyiIntegrated into UniProtKB/TrEMBL: February 7, 2006
Last sequence update: February 7, 2006
Last modified: July 5, 2017
This is version 56 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources