ID   CFI_VERHY               Reviewed;         222 AA.
AC   Q2PF16;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   07-FEB-2006, sequence version 1.
DT   16-JUN-2009, entry version 17.
DE   RecName: Full=Chalcone--flavonone isomerase;
DE            Short=Chalcone isomerase;
DE            EC=5.5.1.6;
GN   Name=CHI;
OS   Verbena hybrida (Garden vervain) (Verbena hortensis).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   asterids; lamiids; Lamiales; Verbenaceae; Glandularia.
OX   NCBI_TaxID=76714;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Temari Violet;
RA   Togami J., Tamura M., Ishiguro K., Hirose C., Okuhara H., Ueyama Y.,
RA   Nakamura N., Yonekura-Sakakibara K., Fukuchi-Mizutani M., Suzuki K.,
RA   Fukui Y., Kusumi T., Tanaka Y.;
RT   "Molecular characterization of the flavonoid biosynthesis of Verbena
RT   hybrida and the functional analysis of verbena and Clitoria ternatea
RT   F3'5'H genes in transgenic verbena.";
RL   Plant Biotechnol. 23:5-11(2006).
CC   -!- FUNCTION: Catalyzes the intramolecular cyclization of bicyclic
CC       chalcones into tricyclic (S)-flavanones. Responsible for the
CC       isomerization of 4,2',4',6'-tetrahydroxychalcone (also termed
CC       chalcone) into naringenin (By similarity).
CC   -!- CATALYTIC ACTIVITY: A chalcone = a flavanone.
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid
CC       biosynthesis.
CC   -!- MISCELLANEOUS: Part of the biosynthetic pathway for all classes of
CC       flavonoids, a large class of secondary plant metabolites, many of
CC       which are brightly colored.
CC   -!- SIMILARITY: Belongs to the chalcone isomerase family.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; AB234907; BAE72880.1; -; mRNA.
DR   BRENDA; 5.5.1.6; 260522.
DR   GO; GO:0045430; F:chalcone isomerase activity; IEA:EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR016088; Chalcone_isomerase_3-sand.
DR   InterPro; IPR003466; Chalcone_isomerase_subgr.
DR   Gene3D; G3DSA:3.50.70.10; Chalcone; 1.
DR   Pfam; PF02431; Chalcone; 1.
PE   2: Evidence at transcript level;
KW   Flavonoid biosynthesis; Isomerase.
FT   CHAIN         1    222       Chalcone--flavonone isomerase.
FT                                /FTId=PRO_0000314575.
FT   BINDING      50     50       Substrate (By similarity).
FT   BINDING     115    115       Substrate (By similarity).
FT   BINDING     192    192       Substrate (By similarity).
FT   SITE        108    108       Important for catalytic activity (By
FT                                similarity).
SQ   SEQUENCE   222 AA;  24020 MW;  A3ADBF59CAB8A46D CRC64;
     MIILPSITEV QVESVVFPPA VKPPGSDKTF FLGGAGVRGL EIEGKFIKFT AIGVYLENDA
     VTSLAAKWKG KTAEEVADSG DFFAEIVTGP FEKFTKVTTI LPLTGQQYSE KVVENCVAYW
     KAIGKFTDAE DEATKKFLQV FKNEMFHPGA SILFTQSPQG SLTISFSKDG SIPEKENAII
     ENKQLSEAVL ESIIGKKGVS PSAKQSLAAR LSDLLKQCES TT
//