Reviewed, UniProtKB/Swiss-Prot Q2PF16 (CFI_VERHY)

Names and origin

Protein names

Recommended name:
    Chalcone--flavonone isomerase
      Short name=Chalcone isomerase
    EC=5.5.1.6

Gene names

Name:

CHI

Organism

Verbena hybrida (Garden vervain) (Verbena hortensis)

Taxonomic identifier

76714 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Lamiales › Verbenaceae › Glandularia

Protein attributes

Sequence length	222 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the intramolecular cyclization of bicyclic chalcones into tricyclic (S)-flavanones. Responsible for the isomerization of 4,2',4',6'-tetrahydroxychalcone (also termed chalcone) into naringenin By similarity.
Catalytic activity	A chalcone = a flavanone.
Pathway	Secondary metabolite biosynthesis; flavonoid biosynthesis.
Miscellaneous	Part of the biosynthetic pathway for all classes of flavonoids, a large class of secondary plant metabolites, many of which are brightly colored.
Sequence similarities	Belongs to the chalcone isomerase family.

Ontologies

Keywords
Biological process	Flavonoid biosynthesis
Molecular function	Isomerase
Gene Ontology (GO)
Biological process	flavonoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	chalcone isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 222	222	Chalcone--flavonone isomerase		PRO_0000314575
Sites
	Binding site	50	1	Substrate By similarity
	Binding site	115	1	Substrate By similarity
	Binding site	192	1	Substrate By similarity
	Site	108	1	Important for catalytic activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2PF16-1 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: A3ADBF59CAB8A46D	FASTA	222	24,020
	10 20 30 40 50 60 MIILPSITEV QVESVVFPPA VKPPGSDKTF FLGGAGVRGL EIEGKFIKFT AIGVYLENDA 70 80 90 100 110 120 VTSLAAKWKG KTAEEVADSG DFFAEIVTGP FEKFTKVTTI LPLTGQQYSE KVVENCVAYW 130 140 150 160 170 180 KAIGKFTDAE DEATKKFLQV FKNEMFHPGA SILFTQSPQG SLTISFSKDG SIPEKENAII 190 200 210 220 ENKQLSEAVL ESIIGKKGVS PSAKQSLAAR LSDLLKQCES TT

References

[1]

"Molecular characterization of the flavonoid biosynthesis of Verbena hybrida and the functional analysis of verbena and Clitoria ternatea F3'5'H genes in transgenic verbena." Togami J., Tamura M., Ishiguro K., Hirose C., Okuhara H., Ueyama Y., Nakamura N., Yonekura-Sakakibara K., Fukuchi-Mizutani M., Suzuki K., Fukui Y., Kusumi T., Tanaka Y. Plant Biotechnol. 23:5-11(2006) Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Temari Violet.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB234907 mRNA. Translation: BAE72880.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	5.5.1.6. 260522.
Family and domain databases
InterPro	IPR016088. Chalcone_isomerase_3-sand. IPR003466. Chalcone_isomerase_subgr. [Graphical view]
Gene3D	G3DSA:3.50.70.10. Chalcone. 1 hit.
Pfam	PF02431. Chalcone. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CFI_VERHY

Accession

Primary (citable) accession number: Q2PF16

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 7, 2006
Last modified:	June 16, 2009
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)