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Protein

Matrix protein VP40

Gene

VP40

Organism
Zaire ebolavirus (strain Gabon-94) (ZEBOV) (Zaire Ebola virus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Promotes virus assembly and budding by interacting with host proteins of the multivesicular body pathway. May facilitate virus budding by interacting with the nucleocapsid and the plasma membrane. Specific interactions with membrane-associated GP and VP24 during the budding process may also occur. The hexamer form seems to be involved in budding. The octamer form binds RNA, and may play a role in genome replication (By similarity).By similarity

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
  2. structural constituent of virion Source: UniProtKB-KW

GO - Biological processi

  1. viral budding via host ESCRT complex Source: UniProtKB-KW
  2. viral release from host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

Host-virus interaction, Viral budding, Viral budding via the host ESCRT complexes, Viral RNA replication, Virus exit from host cell

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Matrix protein VP40
Alternative name(s):
Membrane-associated protein VP40
Gene namesi
Name:VP40
OrganismiZaire ebolavirus (strain Gabon-94) (ZEBOV) (Zaire Ebola virus)
Taxonomic identifieri128947 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesMononegaviralesFiloviridaeEbolavirus
Virus hostiEpomops franqueti (Franquet's epauleted fruit bat) [TaxID: 77231]
Homo sapiens (Human) [TaxID: 9606]
Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]

Subcellular locationi

Virion membrane By similarity; Peripheral membrane protein By similarity. Host endomembrane system By similarity; Peripheral membrane protein By similarity. Host cell membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
Note: In virion, localizes on the intravirional side of the membrane. In the host cell, it is found associated with virus-induced membrane proliferation foci and probably also in multivesicular bodies. These VP40-enriched membrane clusters are then redistributed to the plasma membrane where budding takes place (By similarity).By similarity

GO - Cellular componenti

  1. host cell endomembrane system Source: UniProtKB-SubCell
  2. host cell plasma membrane Source: UniProtKB-SubCell
  3. ribonucleoprotein complex Source: UniProtKB-KW
  4. virion membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral matrix protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 326326Matrix protein VP40PRO_0000245079Add
BLAST

Interactioni

Subunit structurei

Monomer, homo-hexamer or homo-octamer. Membrane association induces conformational switch from monomer to hexamer. Interacts with host TSG101. Homo-hexamer interacts with the WW domain 3 of host NEDD4. Interacts with the nucleoprotein. May interact with VP35 (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ2PDK5.
SMRiQ2PDK5. Positions 44-321.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni212 – 2143Important for oligomerizationBy similarity
Regioni213 – 326114Membrane-bindingBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi7 – 104PTAP/PSAP motif
Motifi10 – 134PPXY motif

Domaini

Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. VP40 contains two overlapping L domains: a PTAP/PSAP motif, which interacts with the UEV domain of TSG101 and a PPXY motif which interacts with the WW domain 3 of NEDD4.

Sequence similaritiesi

Family and domain databases

InterProiIPR008986. EV_matrix.
[Graphical view]
PfamiPF07447. VP40. 1 hit.
[Graphical view]
PIRSFiPIRSF018327. VP40_FiloV. 1 hit.
SUPFAMiSSF50012. SSF50012. 2 hits.

Sequencei

Sequence statusi: Complete.

Q2PDK5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRVILPTAP PEYMEAIYPV RSNSTIARGG NSNTGFLTPE SVNGDTPSNP
60 70 80 90 100
LRPIADDTID HASHTPGSVS SAFILEAMVN VISGPKVLMK QIPIWLPLGV
110 120 130 140 150
ADQKTYSFDS TTAAIMLASY TITHFGKATN PLVRVNRLGP GIPDHPLRLL
160 170 180 190 200
RIGNQAFLQE FVLPPVQLPQ YFTFDLTALK LITQPLPAAT WTDDTPTGSN
210 220 230 240 250
GALRPGISFH PKLRPILLPN KSGKKGNSAD LTFPEKIQAI MTSLQDLKIV
260 270 280 290 300
PIDPTKNIMG IEVPETLVHK LTGKKVTSKN GQPIIPVLLP KYIGLDPVAP
310 320
GDLTMVITQD CDTCHSPASL PAVIEK
Length:326
Mass (Da):35,209
Last modified:February 6, 2006 - v1
Checksum:i8C67320BA6FAD51F
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti65 – 651T → I in strain: Isolate Bouee-96.
Natural varianti233 – 2331F → S in strain: Isolate Bouee-96.
Natural varianti247 – 2471L → F in strain: Isolate Bouee-96.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ001707 mRNA. Translation: CAC79598.1.
AY058896 mRNA. Translation: AAL25816.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ001707 mRNA. Translation: CAC79598.1.
AY058896 mRNA. Translation: AAL25816.1.

3D structure databases

ProteinModelPortaliQ2PDK5.
SMRiQ2PDK5. Positions 44-321.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR008986. EV_matrix.
[Graphical view]
PfamiPF07447. VP40. 1 hit.
[Graphical view]
PIRSFiPIRSF018327. VP40_FiloV. 1 hit.
SUPFAMiSSF50012. SSF50012. 2 hits.
ProtoNetiSearch...

Publicationsi

  1. "VP40 of Ebola virus Gabon 94 strain."
    Prehaud C.J.
    Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Sequence analysis of the GP, NP, VP40 and VP24 genes of Ebola virus isolated from deceased, surviving and asymptomatically infected individuals during the 1996 outbreak in Gabon: comparative studies and phylogenetic characterization."
    Leroy E.M., Baize S., Mavoungou E., Apetrei C.
    J. Gen. Virol. 83:67-73(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Isolate Bouee-96.

Entry informationi

Entry nameiVP40_EBOG4
AccessioniPrimary (citable) accession number: Q2PDK5
Secondary accession number(s): Q913A4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2006
Last sequence update: February 6, 2006
Last modified: February 3, 2015
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Most abundant protein in the virion.By similarity

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.