Q2PDK5 (VP40_EBOG4) Reviewed, UniProtKB/Swiss-Prot
Last modified February 19, 2014. Version 44. History...
Names and origin
|Protein names||Recommended name:|
Matrix protein VP40
Membrane-associated protein VP40
|Organism||Zaire ebolavirus (strain Gabon-94) (ZEBOV) (Zaire Ebola virus)|
|Taxonomic identifier||128947 [NCBI]|
|Taxonomic lineage||Viruses › ssRNA negative-strand viruses › Mononegavirales › Filoviridae › Ebolavirus ›|
|Virus host||Epomops franqueti (Franquet's epauleted fruit bat) [TaxID: 77231]|
Homo sapiens (Human) [TaxID: 9606]
Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]
|Sequence length||326 AA.|
|Protein existence||Evidence at transcript level|
General annotation (Comments)
Promotes virus assembly and budding by interacting with host proteins of the multivesicular body pathway. May facilitate virus budding by interacting with the nucleocapsid and the plasma membrane. Specific interactions with membrane-associated GP and VP24 during the budding process may also occur. The hexamer form seems to be involved in budding. The octamer form binds RNA, and may play a role in genome replication By similarity.
Monomer, homo-hexamer or homo-octamer. Membrane association induces conformational switch from monomer to hexamer. Interacts with host TSG101. Homo-hexamer interacts with the WW domain 3 of host NEDD4. Interacts with the nucleoprotein. May interact with VP35 By similarity.
Virion membrane; Peripheral membrane protein By similarity. Host endomembrane system; Peripheral membrane protein By similarity. Host cell membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Note: In virion, localizes on the intravirional side of the membrane. In the host cell, it is found associated with virus-induced membrane proliferation foci and probably also in multivesicular bodies. These VP40-enriched membrane clusters are then redistributed to the plasma membrane where budding takes place By similarity.
Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. VP40 contains two overlapping L domains: a PTAP/PSAP motif, which interacts with the UEV domain of TSG101 and a PPXY motif which interacts with the WW domain 3 of NEDD4.
Most abundant protein in the virion By similarity.
Belongs to the filoviridae matrix protein VP40 family.
Sequence annotation (Features)
|Feature key||Position(s)||Length||Description||Graphical view||Feature identifier|
|Chain||1 – 326||326||Matrix protein VP40||PRO_0000245079|
|Region||212 – 214||3||Important for oligomerization By similarity|
|Region||213 – 326||114||Membrane-binding By similarity|
|Motif||7 – 10||4||PTAP/PSAP motif|
|Motif||10 – 13||4||PPXY motif|
|Natural variant||65||1||T → I in strain: Isolate Bouee-96. |
|Natural variant||233||1||F → S in strain: Isolate Bouee-96. |
|Natural variant||247||1||L → F in strain: Isolate Bouee-96. |
|||"VP40 of Ebola virus Gabon 94 strain."|
Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
|||"Sequence analysis of the GP, NP, VP40 and VP24 genes of Ebola virus isolated from deceased, surviving and asymptomatically infected individuals during the 1996 outbreak in Gabon: comparative studies and phylogenetic characterization."|
Leroy E.M., Baize S., Mavoungou E., Apetrei C.
J. Gen. Virol. 83:67-73(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Isolate Bouee-96.
|AJ001707 mRNA. Translation: CAC79598.1.|
AY058896 mRNA. Translation: AAL25816.1.
3D structure databases
|SMR||Q2PDK5. Positions 44-321. |
Protocols and materials databases
Family and domain databases
|InterPro||IPR008986. EV_matrix. |
|Pfam||PF07447. VP40. 1 hit. |
|PIRSF||PIRSF018327. VP40_FiloV. 1 hit. |
|SUPFAM||SSF50012. SSF50012. 2 hits. |
|Accession||Primary (citable) accession number: Q2PDK5|
Secondary accession number(s): Q913A4
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Viral Protein Annotation Program|
Index of protein domains and families