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Q2PDK5

- VP40_EBOG4

UniProt

Q2PDK5 - VP40_EBOG4

Protein

Matrix protein VP40

Gene

VP40

Organism
Zaire ebolavirus (strain Gabon-94) (ZEBOV) (Zaire Ebola virus)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 45 (01 Oct 2014)
      Sequence version 1 (07 Feb 2006)
      Previous versions | rss
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    Functioni

    Promotes virus assembly and budding by interacting with host proteins of the multivesicular body pathway. May facilitate virus budding by interacting with the nucleocapsid and the plasma membrane. Specific interactions with membrane-associated GP and VP24 during the budding process may also occur. The hexamer form seems to be involved in budding. The octamer form binds RNA, and may play a role in genome replication By similarity.By similarity

    GO - Molecular functioni

    1. RNA binding Source: UniProtKB-KW
    2. structural constituent of virion Source: UniProtKB-KW

    GO - Biological processi

    1. viral budding via host ESCRT complex Source: UniProtKB-KW
    2. viral release from host cell Source: UniProtKB-KW

    Keywords - Molecular functioni

    Ribonucleoprotein

    Keywords - Biological processi

    Host-virus interaction, Viral budding, Viral budding via the host ESCRT complexes, Viral RNA replication, Virus exit from host cell

    Keywords - Ligandi

    RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Matrix protein VP40
    Alternative name(s):
    Membrane-associated protein VP40
    Gene namesi
    Name:VP40
    OrganismiZaire ebolavirus (strain Gabon-94) (ZEBOV) (Zaire Ebola virus)
    Taxonomic identifieri128947 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesMononegaviralesFiloviridaeEbolavirus
    Virus hostiEpomops franqueti (Franquet's epauleted fruit bat) [TaxID: 77231]
    Homo sapiens (Human) [TaxID: 9606]
    Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]

    Subcellular locationi

    Virion membrane By similarity; Peripheral membrane protein By similarity. Host endomembrane system By similarity; Peripheral membrane protein By similarity. Host cell membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
    Note: In virion, localizes on the intravirional side of the membrane. In the host cell, it is found associated with virus-induced membrane proliferation foci and probably also in multivesicular bodies. These VP40-enriched membrane clusters are then redistributed to the plasma membrane where budding takes place By similarity.By similarity

    GO - Cellular componenti

    1. host cell endomembrane system Source: UniProtKB-SubCell
    2. host cell plasma membrane Source: UniProtKB-SubCell
    3. ribonucleoprotein complex Source: UniProtKB-KW
    4. virion membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cell membrane, Host membrane, Membrane, Viral matrix protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 326326Matrix protein VP40PRO_0000245079Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer, homo-hexamer or homo-octamer. Membrane association induces conformational switch from monomer to hexamer. Interacts with host TSG101. Homo-hexamer interacts with the WW domain 3 of host NEDD4. Interacts with the nucleoprotein. May interact with VP35 By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ2PDK5.
    SMRiQ2PDK5. Positions 44-321.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni212 – 2143Important for oligomerizationBy similarity
    Regioni213 – 326114Membrane-bindingBy similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi7 – 104PTAP/PSAP motif
    Motifi10 – 134PPXY motif

    Domaini

    Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. VP40 contains two overlapping L domains: a PTAP/PSAP motif, which interacts with the UEV domain of TSG101 and a PPXY motif which interacts with the WW domain 3 of NEDD4.

    Sequence similaritiesi

    Family and domain databases

    InterProiIPR008986. EV_matrix.
    [Graphical view]
    PfamiPF07447. VP40. 1 hit.
    [Graphical view]
    PIRSFiPIRSF018327. VP40_FiloV. 1 hit.
    SUPFAMiSSF50012. SSF50012. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Q2PDK5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRRVILPTAP PEYMEAIYPV RSNSTIARGG NSNTGFLTPE SVNGDTPSNP    50
    LRPIADDTID HASHTPGSVS SAFILEAMVN VISGPKVLMK QIPIWLPLGV 100
    ADQKTYSFDS TTAAIMLASY TITHFGKATN PLVRVNRLGP GIPDHPLRLL 150
    RIGNQAFLQE FVLPPVQLPQ YFTFDLTALK LITQPLPAAT WTDDTPTGSN 200
    GALRPGISFH PKLRPILLPN KSGKKGNSAD LTFPEKIQAI MTSLQDLKIV 250
    PIDPTKNIMG IEVPETLVHK LTGKKVTSKN GQPIIPVLLP KYIGLDPVAP 300
    GDLTMVITQD CDTCHSPASL PAVIEK 326
    Length:326
    Mass (Da):35,209
    Last modified:February 7, 2006 - v1
    Checksum:i8C67320BA6FAD51F
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti65 – 651T → I in strain: Isolate Bouee-96.
    Natural varianti233 – 2331F → S in strain: Isolate Bouee-96.
    Natural varianti247 – 2471L → F in strain: Isolate Bouee-96.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ001707 mRNA. Translation: CAC79598.1.
    AY058896 mRNA. Translation: AAL25816.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ001707 mRNA. Translation: CAC79598.1 .
    AY058896 mRNA. Translation: AAL25816.1 .

    3D structure databases

    ProteinModelPortali Q2PDK5.
    SMRi Q2PDK5. Positions 44-321.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR008986. EV_matrix.
    [Graphical view ]
    Pfami PF07447. VP40. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF018327. VP40_FiloV. 1 hit.
    SUPFAMi SSF50012. SSF50012. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "VP40 of Ebola virus Gabon 94 strain."
      Prehaud C.J.
      Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Sequence analysis of the GP, NP, VP40 and VP24 genes of Ebola virus isolated from deceased, surviving and asymptomatically infected individuals during the 1996 outbreak in Gabon: comparative studies and phylogenetic characterization."
      Leroy E.M., Baize S., Mavoungou E., Apetrei C.
      J. Gen. Virol. 83:67-73(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Isolate Bouee-96.

    Entry informationi

    Entry nameiVP40_EBOG4
    AccessioniPrimary (citable) accession number: Q2PDK5
    Secondary accession number(s): Q913A4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 11, 2006
    Last sequence update: February 7, 2006
    Last modified: October 1, 2014
    This is version 45 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Most abundant protein in the virion.By similarity

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3