Q2PC20 (PPM1K_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein phosphatase 1K, mitochondrial
EC=3.1.3.16

Alternative name(s):
Protein phosphatase 2C isoform kappa
Short name=PP2C-kappa

Gene names

Name:

PPM1K

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Regulates the mitochondrial permeability transition pore and is essential for cellular survival and development By similarity.
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the PP2C family. Contains 1 PP2C-like domain.

Ontologies

Keywords
Cellular component	Mitochondrion
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Ligand	Magnesium Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein dephosphorylation Inferred from electronic annotation. Source: InterPro
Cellular_component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine phosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q2PC20-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q2PC20-2)

The sequence of this isoform differs from the canonical sequence as follows:
     106-153: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 29	29	Mitochondrion By similarity
	Chain	30 – 372	343	Protein phosphatase 1K, mitochondrial		PRO_0000278207
Regions
	Domain	93 – 346	254	PP2C-like
Sites
	Metal binding	127	1	Magnesium By similarity
	Metal binding	128	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	337	1	Magnesium By similarity
Natural variations
	Alternative sequence	106 – 153	48	Missing in isoform 2.		VSP_023155
Experimental info
	Sequence conflict	365	1	F → I in AAI18080. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 5A8536B6E2F78090	FASTA	372	41,151
	10 20 30 40 50 60 MSTAALLTLV RSGGNQVRRR VLLRARGLQD DRWVMPTCHS STSEPKWSRF DPDGSGRPAT 70 80 90 100 110 120 WDNFGIWDNR LEEPILLPPS IKYGKPIPKV SLQNVGSASQ IGKRKENEDR FGFAQLTNEV 130 140 150 160 170 180 LYFAVYDGHG GPAAADFCHT HMEKCILDLL PKEENLETVL TLAFLEIDKT FARHAHLSAD 190 200 210 220 230 240 ATLLTSGTTA TVALLRDGIE LVIASVGDSR AILCRKGKPM KLTIDHTPER KDEKERIKKC 250 260 270 280 290 300 GGFVAWNSLG QPHVNGRLAM TRSLGDLDLK TSGVIAEPET KRIKLHHADD SFLVLTTDGI 310 320 330 340 350 360 NFMVNSQEIC DFVNQCHDPN EAAHAVTEQA IQYGTEDNTT AVVVPFGAWG KYKNSEITFS 370 FSRSFASSGR WA
	Isoform 2 [UniParc]. Checksum: 452CFAA721E6D8B8	FASTA	324	35,755
	10 20 30 40 50 60 MSTAALLTLV RSGGNQVRRR VLLRARGLQD DRWVMPTCHS STSEPKWSRF DPDGSGRPAT 70 80 90 100 110 120 WDNFGIWDNR LEEPILLPPS IKYGKPIPKV SLQNVGSASQ IGKRKENLET VLTLAFLEID 130 140 150 160 170 180 KTFARHAHLS ADATLLTSGT TATVALLRDG IELVIASVGD SRAILCRKGK PMKLTIDHTP 190 200 210 220 230 240 ERKDEKERIK KCGGFVAWNS LGQPHVNGRL AMTRSLGDLD LKTSGVIAEP ETKRIKLHHA 250 260 270 280 290 300 DDSFLVLTTD GINFMVNSQE ICDFVNQCHD PNEAAHAVTE QAIQYGTEDN TTAVVVPFGA 310 320 WGKYKNSEIT FSFSRSFASS GRWA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Seroussi E. Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2). Strain: Holstein.
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: Hereford. Tissue: Hypothalamus.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ871967 mRNA. Translation: CAI44748.1. AJ871968 mRNA. Translation: CAI44749.1. BC118079 mRNA. Translation: AAI18080.1.
IPI	IPI00689389. IPI00829546.
RefSeq	NP_001039939.1. NM_001046474.2.
UniGene	Bt.48380.
3D structure databases
ProteinModelPortal	Q2PC20.
SMR	Q2PC20. Positions 90-349.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000007563; ENSBTAP00000007563; ENSBTAG00000005754.
GeneID	540329.
KEGG	bta:540329.
Organism-specific databases
CTD	152926.
Phylogenomic databases
eggNOG	COG0631.
GeneTree	ENSGT00690000101775.
HOGENOM	HOG000059620.
HOVERGEN	HBG096199.
InParanoid	Q2PC20.
OMA	GCASHIG.
OrthoDB	EOG4MCX0J.
Family and domain databases
Gene3D	3.60.40.10. 1 hit.
InterPro	IPR001932. PP2C-like. IPR000222. PP2C_Mn2_Asp60_BS. IPR015655. Protein_Pase_2C. [Graphical view]
PANTHER	PTHR13832. PTHR13832. 1 hit.
Pfam	PF00481. PP2C. 1 hit. [Graphical view]
SMART	SM00331. PP2C_SIG. 1 hit. SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF81606. PP2C-related. 1 hit.
PROSITE	PS01032. PP2C. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20878566.

Entry information

Entry name

PPM1K_BOVIN

Accession

Primary (citable) accession number: Q2PC20
Secondary accession number(s): Q17R15, Q2PC19

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	February 7, 2006
Last modified:	March 6, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program