Reviewed, UniProtKB/Swiss-Prot Q2P9F7 (GLGB1_XANOM)

Names and origin

Protein names

Recommended name:
    1,4-alpha-glucan-branching enzyme 1
    EC=2.4.1.18
Alternative name(s):
    Glycogen-branching enzyme 1
      Short name=BE 1
    1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase 1

Gene names

Name:	glgB1
Ordered Locus Names:	XOO0065

Organism

Xanthomonas oryzae pv. oryzae (strain MAFF 311018) [Complete proteome] [HAMAP]

Taxonomic identifier

342109 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xanthomonas

Protein attributes

Sequence length	744 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity. HAMAP MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Glycogen biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 744	744	1,4-alpha-glucan-branching enzyme 1 HAMAP MF_00685		PRO_0000260718
Sites
	Active site	317	1	By similarity
	Active site	352	1	By similarity
	Active site	357	1	By similarity
	Active site	420	1	By similarity
	Active site	422	1	By similarity
	Active site	475	1	By similarity
	Active site	542	1	By similarity
	Active site	543	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2P9F7-1 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: F12478404DC553B7	FASTA	744	82,101
	10 20 30 40 50 60 MSERQGGQEQ DREADGVTTE GISEMSQTLQ ALADGLPVDA FAVLGPHPLA DGRRQVRVLA 70 80 90 100 110 120 PGAEAMGLID SRGKLLARMQ ASAIDGVFEG ILSIEGPYRL RIVWPDMVQE IEDPYAFAVT 130 140 150 160 170 180 LDESLLLQIA AGDGQALRRA LGAQHVQCGE VPGVRFAVWA PHAQRVAVVG DFNGWDVRRH 190 200 210 220 230 240 PMRQRIGGFW ELFLPRVEAG PRYKYAVTAA DGRVLLKADP VARQTELPPA TASVVPGTDT 250 260 270 280 290 300 FAWTDAAWMA KRDPSAVPAP LSIYEVHAAS WRRDGHNQPL DWPTLAEQLI PYVQQLRFTH 310 320 330 340 350 360 IELLPITEHP FGGSWGYQPL GLYAPTARHG SPDGFAQFVD ACHRAGIGVI LDWVSAHFPD 370 380 390 400 410 420 DAHGLAQFDG AALYEHADPR EGMHRDWNTL IYNYGRPEVT AYLLGSALEW IDHYHLDGLR 430 440 450 460 470 480 VDAVASMLYR DYGRAEGEWV PNAHGGRENL EAVAFLRQLN REIAAHFPGV LTIAEESTAW 490 500 510 520 530 540 PGVTAAISDG GLGFTHKWNM GWMHDTLSYM QRDPAERAHH HSQLTFGLVY AFDERFVLPI 550 560 570 580 590 600 SHDEVVHGTG GLLGQMPGDD WRRFANLRAY LALMWAHPGD KLLFMGAEFG QWADWNHDQS 610 620 630 640 650 660 LDWHLLEGAR HRGVQLLVGD LNATLRRTPA LYRGTHRAKG FDWSVADDAR NSVLAFIRHD 670 680 690 700 710 720 PDGGGVPLLA VSNLTAQPLH DYGVGVPRAG AWREILNTDS AHYGGSNLGN SGRLATEPMG 730 740 MHGHAQRLRL TLPPLATIYL QAEK

References

[1]

"Genome sequence of Xanthomonas oryzae pv. oryzae suggests contribution of large numbers of effector genes and insertion sequences to its race diversity." Ochiai H., Inoue Y., Takeya M., Sasaki A., Kaku H. Jpn. Agric. Res. Q. 39:275-287(2005) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP008229 Genomic DNA. Translation: BAE66820.1.
RefSeq	YP_449094.1.
3D structure databases
SMR	Q2P9F7. Positions 139-744.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2P9F7.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Genome annotation databases
GeneID	3859717.
GenomeReviews	Gene locus XOO0065 in contig AP008229_GR.
KEGG	xom:XOO_0065.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0296.
HOGENOM	HBG287139.
OMA	GFDWSVA.
PhylomeDB	Q2P9F7.
Enzyme and pathway databases
BioCyc	XORY342109:XOO0065-MONOMER.
Family and domain databases
HAMAP	MF_00685. GlgB. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz_core. IPR006048. A-amylase_b_C. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR017853. Glyco_hydro_catalytic_core. IPR013781. Glyco_hydro_sg_catalytic. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
TIGRFAMs	TIGR01515. branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB1_XANOM

Accession

Primary (citable) accession number: Q2P9F7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	February 7, 2006
Last modified:	February 9, 2010
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)