Reviewed, UniProtKB/Swiss-Prot Q2P949 (GLGB2_XANOM)

Names and origin

Protein names

Recommended name:
    1,4-alpha-glucan-branching enzyme 2
    EC=2.4.1.18
Alternative name(s):
    Glycogen-branching enzyme 2
      Short name=BE 2
    1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase 2

Gene names

Name:	glgB2
Ordered Locus Names:	XOO0173

Organism

Xanthomonas oryzae pv. oryzae (strain MAFF 311018) [Complete proteome] [HAMAP]

Taxonomic identifier

342109 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xanthomonas

Protein attributes

Sequence length	731 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity.
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Glycogen biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 731	731	1,4-alpha-glucan-branching enzyme 2 HAMAP MF_00685		PRO_0000260719
Sites
	Active site	305	1	By similarity
	Active site	340	1	By similarity
	Active site	345	1	By similarity
	Active site	408	1	By similarity
	Active site	410	1	By similarity
	Active site	463	1	By similarity
	Active site	530	1	By similarity
	Active site	531	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2P949-1 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 661BA6EE50D4FAAA	FASTA	731	80,781
	10 20 30 40 50 60 MSGVMTAVSN RWDPGVIRAL AEARHGDAFA VLGAHRTDTG RVLRTYLPGA ERVSAVLDDG 70 80 90 100 110 120 QTIALEAGPE PGLFAGDLPA QGGYRLRIGW PGGEQDTADP YAFGPQLSDF DLHLISEGHH 130 140 150 160 170 180 LQLADALGAN VVEVDGVRGT RFAVWAPNAS RVAVVGDFNS WDARRHPMRL RHQSGVWELF 190 200 210 220 230 240 VPDVGPGAHY KYQLRGPHGH ELPAKADPVA RRAELAPGTA SIVADPTPYQ WSDDGWMATR 250 260 270 280 290 300 ARRQAHDAPM SVYEMHAGSW LREEGVDLDW DGLADRLIPY VADMGFTHVE LMPVTEHPFG 310 320 330 340 350 360 GSWGYQPLGL FAPTARFGTP DGFARFVDRC HREGIGVIVD WVPAHFPTDA HGLAHFDGTA 370 380 390 400 410 420 LYEHADPREG FHRDWNTLIY NHGRREVSGF LIASAMEFLQ RYHVDGLRVD AVASMLYRDY 430 440 450 460 470 480 SRNAGEWVPN IHGGRENYET IAFLRRLNAL VREHTPGAVM IAEESTAFPG VTADVAHGGL 490 500 510 520 530 540 GFHYKWNMGW MHDTLHYAGL DPIYRRYHHG ELTFSMVYAY SERFVLPISH DEVVHGKGSL 550 560 570 580 590 600 LGRMPGDDWQ RFANLRAYLG FMFTHPGRKL LFMGCEFGQP TEWNHDSGLP WHLLDDPRHR 610 620 630 640 650 660 GVQTLVRDVN RLYVQYPALH AHDDDPSGFA WVVGDDAGNS VVAFLRKGKR GDAPVLVVIN 670 680 690 700 710 720 FTPVVQHGYR IGVPQGGQWR EVFNSDAGIY GGANLGNGGI VTAEQQSMHG HAHALPLLLP 730 PLGAIVLTPP G

References

[1]

"Genome sequence of Xanthomonas oryzae pv. oryzae suggests contribution of large numbers of effector genes and insertion sequences to its race diversity." Ochiai H., Inoue Y., Takeya M., Sasaki A., Kaku H. Jpn. Agric. Res. Q. 39:275-287(2005) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP008229 Genomic DNA. Translation: BAE66928.1.
RefSeq	YP_449202.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q2P949.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Genome annotation databases
GeneID	3859276.
GenomeReviews	Gene locus XOO0173 in contig AP008229_GR.
KEGG	xom:XOO_0173.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2P949.
OMA	PKTASIV.
Enzyme and pathway databases
BioCyc	XORY342109:XOO0173-MON.
Family and domain databases
HAMAP	MF_00685. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz_core. IPR006048. A-amylase_b_C. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_sg_catalytic. IPR013783. Ig-like_fold. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 2 hits. [Graphical view]
TIGRFAMs	TIGR01515. branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB2_XANOM

Accession

Primary (citable) accession number: Q2P949

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	February 7, 2006
Last modified:	November 3, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)