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Protein

Pimeloyl-[acyl-carrier protein] methyl ester esterase

Gene

bioH

Organism
Xanthomonas oryzae pv. oryzae (strain MAFF 311018)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The physiological role of BioH is to remove the methyl group introduced by BioC when the pimeloyl moiety is complete. It allows to synthesize pimeloyl-ACP via the fatty acid synthetic pathway through the hydrolysis of the ester bonds of pimeloyl-ACP esters.UniRule annotation

Catalytic activityi

Pimeloyl-[acyl-carrier protein] methyl ester + H2O = pimeloyl-[acyl-carrier protein] + methanol.UniRule annotation

Pathwayi: biotin biosynthesis

This protein is involved in the pathway biotin biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei18 – 181Substrate; via amide nitrogen and carbonyl oxygenUniRule annotation
Active sitei78 – 781NucleophileUniRule annotation
Active sitei203 – 2031UniRule annotation
Active sitei231 – 2311UniRule annotation
Binding sitei231 – 2311SubstrateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Keywords - Biological processi

Biotin biosynthesis

Enzyme and pathway databases

BioCyciXORY342109:GIX9-215-MONOMER.
UniPathwayiUPA00078.

Protein family/group databases

ESTHERixanor-bioh. BioH.
MEROPSiS33.994.

Names & Taxonomyi

Protein namesi
Recommended name:
Pimeloyl-[acyl-carrier protein] methyl ester esteraseUniRule annotation (EC:3.1.1.85UniRule annotation)
Alternative name(s):
Biotin synthesis protein BioHUniRule annotation
Carboxylesterase BioHUniRule annotation
Gene namesi
Name:bioHUniRule annotation
Ordered Locus Names:XOO0215
OrganismiXanthomonas oryzae pv. oryzae (strain MAFF 311018)
Taxonomic identifieri342109 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 253253Pimeloyl-[acyl-carrier protein] methyl ester esterasePRO_1000067282Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ2P907.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni78 – 792Substrate bindingUniRule annotation
Regioni139 – 1435Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000253140.
KOiK02170.
OMAiTIGEGDC.
OrthoDBiEOG6FJNH9.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
HAMAPiMF_01260. Carboxylester.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR010076. BioH.
[Graphical view]
PfamiPF12697. Abhydrolase_6. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
TIGRFAMsiTIGR01738. bioH. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2P907-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHIDVIGHGP ALVLLHGWAL HGGVFAPLVE RLAPHYQLHL VDLPGHGFSR
60 70 80 90 100
DDSTPLALPY VVAEIAAATP PAVWLGWSLG GLFALHAAAT LPQVRGLAMI
110 120 130 140 150
AATPRFVRGS DWPDAVQREL FVQFGTELSR DYRGTLERFL ALDTLGSAHA
160 170 180 190 200
RSELRSLRET LTARGEPAPE ALQQGLSLLE RTDLRRALPQ LARPSLWIAG
210 220 230 240 250
QRDRLVPAAG MHAAAALSPH AQALTIAGGG HAPFLGHADQ VSEALQRFVA

SVP
Length:253
Mass (Da):27,082
Last modified:February 7, 2006 - v1
Checksum:i2486169AC82E41AF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008229 Genomic DNA. Translation: BAE66970.1.
RefSeqiWP_011407292.1. NC_007705.1.

Genome annotation databases

EnsemblBacteriaiBAE66970; BAE66970; BAE66970.
GeneIDi3264089.
KEGGixom:XOO0215.
PATRICi24109544. VBIXanOry49434_0232.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008229 Genomic DNA. Translation: BAE66970.1.
RefSeqiWP_011407292.1. NC_007705.1.

3D structure databases

ProteinModelPortaliQ2P907.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

ESTHERixanor-bioh. BioH.
MEROPSiS33.994.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAE66970; BAE66970; BAE66970.
GeneIDi3264089.
KEGGixom:XOO0215.
PATRICi24109544. VBIXanOry49434_0232.

Phylogenomic databases

HOGENOMiHOG000253140.
KOiK02170.
OMAiTIGEGDC.
OrthoDBiEOG6FJNH9.

Enzyme and pathway databases

UniPathwayiUPA00078.
BioCyciXORY342109:GIX9-215-MONOMER.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
HAMAPiMF_01260. Carboxylester.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR010076. BioH.
[Graphical view]
PfamiPF12697. Abhydrolase_6. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
TIGRFAMsiTIGR01738. bioH. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of Xanthomonas oryzae pv. oryzae suggests contribution of large numbers of effector genes and insertion sequences to its race diversity."
    Ochiai H., Inoue Y., Takeya M., Sasaki A., Kaku H.
    Jpn. Agric. Res. Q. 39:275-287(2005)
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MAFF 311018.

Entry informationi

Entry nameiBIOH_XANOM
AccessioniPrimary (citable) accession number: Q2P907
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: February 7, 2006
Last modified: July 6, 2016
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.