Reviewed, UniProtKB/Swiss-Prot Q2P5Q6 (CHEB1_XANOM)

Names and origin

Protein names

Recommended name:
    Chemotaxis response regulator protein-glutamate methylesterase 1
    EC=3.1.1.61

Gene names

Name:	cheB1
Ordered Locus Names:	XOO1366

Organism

Xanthomonas oryzae pv. oryzae (strain MAFF 311018) [Complete proteome] [HAMAP]

Taxonomic identifier

342109 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xanthomonas

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by cheR By similarity. HAMAP MF_00099
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm HAMAP MF_00099.
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by cheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity. HAMAP MF_00099
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: HAMAP regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro sensory perception of chemical stimulus Inferred from electronic annotation. Source: HAMAP two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: HAMAP two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 351	351	Chemotaxis response regulator protein-glutamate methylesterase 1 HAMAP MF_00099		PRO_0000264333
Regions
	Domain	1 – 115	115	Response regulatory
	Domain	161 – 351	191	CheB-type methylesterase
Sites
	Active site	173	1	By similarity
	Active site	199	1	By similarity
	Active site	295	1	By similarity
Amino acid modifications
	Modified residue	49	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2P5Q6-1 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 980FA18F3C3AEF56	FASTA	351	37,242
	10 20 30 40 50 60 MVDDSAVVRQ VLVNVLNDAA DIEVIATAAD PLLAIEKMRK QWPDVIVLDV EMPRMDGITF 70 80 90 100 110 120 LRKIMSERPT PVVICSTLTE KGARVTMDAL AAGAVAVVTK PRLGLKQFLT ESADELVATV 130 140 150 160 170 180 RSAARANVKR LAARVTAAPL EAEVKHTADV ILPAQSGRAM AQTTERIVAI GTSTGGTQAL 190 200 210 220 230 240 EEVLTALPRV CPGIVIVQHM PEKFTAAFAA RLNGLCQIAV KEAANNDRVM PGRALIAPGG 250 260 270 280 290 300 KHLLLRRSGA QYFVEVLEGP PVNRHRPSVD VLFRSAARAA GSNALGIIMT GMGDDGAAGL 310 320 330 340 350 LEMRQAGART VAQDEQTSIV FGMPKEAIKR GGADRILALG AMAREIVTQL Q

References

[1]

"Genome sequence of Xanthomonas oryzae pv. oryzae suggests contribution of large numbers of effector genes and insertion sequences to its race diversity." Ochiai H., Inoue Y., Takeya M., Sasaki A., Kaku H. Jpn. Agric. Res. Q. 39:275-287(2005) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP008229 Genomic DNA. Translation: BAE68121.1.
RefSeq	YP_450395.2.
3D structure databases
SMR	Q2P5Q6. Positions 1-350.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2P5Q6.
Genome annotation databases
GeneID	3857499.
GenomeReviews	Gene locus XOO1366 in contig AP008229_GR.
KEGG	xom:XOO_1366.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2201.
HOGENOM	HBG705324.
OMA	PGGKHML.
PhylomeDB	Q2P5Q6.
Enzyme and pathway databases
BioCyc	XORY342109:XOO1366-MONOMER.
Family and domain databases
HAMAP	MF_00099. CheB_methylest. [Tree]
InterPro	IPR011006. CheY-like. IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
SMART	SM00448. REC. 1 hit. [Graphical view]
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB1_XANOM

Accession

Primary (citable) accession number: Q2P5Q6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	February 7, 2006
Last modified:	January 19, 2010
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)