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Q2P5B0

- SYI_XANOM

UniProt

Q2P5B0 - SYI_XANOM

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Xanthomonas oryzae pv. oryzae (strain MAFF 311018)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei577 – 5771Aminoacyl-adenylateUniRule annotation
Binding sitei621 – 6211ATPUniRule annotation
Metal bindingi906 – 9061ZincUniRule annotation
Metal bindingi909 – 9091ZincUniRule annotation
Metal bindingi926 – 9261ZincUniRule annotation
Metal bindingi929 – 9291ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciXORY342109:GIX9-1536-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:XOO1512
OrganismiXanthomonas oryzae pv. oryzae (strain MAFF 311018)
Taxonomic identifieri342109 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas
ProteomesiUP000001957: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 943943Isoleucine--tRNA ligasePRO_1000022142Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi342109.XOO_1512.

Structurei

3D structure databases

ProteinModelPortaliQ2P5B0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi59 – 6911"HIGH" regionAdd
BLAST
Motifi618 – 6225"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2P5B0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTQDYKATLH LPATEFPMRG DLPKREPAML ERWEREGFYA QLRANAAGRP
60 70 80 90 100
LFVLHDGPPY ANGQIHLGHA VNKILKDIIV KSKCLAGFDA PYIPGWDCHG
110 120 130 140 150
LPIEIAIEKK YGKVGVKLDA AEFRQKCREY ATEQIDLQRR DFKRLGVIGD
160 170 180 190 200
WDNPYKTLDF RFEANEIRAL AKVVDNGHLT RGVKPVHWCF DCGSALAEAE
210 220 230 240 250
IEYADKVSPT VDIAYPARDP GAVAAAFGAT LPGGVGVAVP IWTTTPWTLP
260 270 280 290 300
ASLAVSLGAE LDYVLVEGPA DRGQPRWLVI AEALAAKALA RYGVDEVVVH
310 320 330 340 350
GHAKGTALEQ MLLNHPFYAE REIPLLLGDH VSAEDGTGAV HTAPGHGQED
360 370 380 390 400
YQVSKQYGLL ERYGAAQINP VDGRGVYLPS TPPLGDTVLA GLHIWKANDV
410 420 430 440 450
IIEALHGTGV LLAASKMEHS YPHCWRHKTP IAFRATPQWF ISMEQANLRA
460 470 480 490 500
DALKAIESVH WYPSWGQARI AGMVDGRPDW TISRQRTWGV PIALFVHRET
510 520 530 540 550
GEPHPRSTEL LRQVADRVEL GGVDVWYTLD AAELLGDEAA DYDKITDILD
560 570 580 590 600
VWFDSGVTHE AVLVDRGLPK PADLYLEGSD QHRGWFQSSL LSGVAMDKAA
610 620 630 640 650
PYKQCLTHGF TVDEHGRKMS KSLGNGIEPQ DIMKTLGADI LRLWIASADY
660 670 680 690 700
SNEMSLSQEI LKRNADAYRR LRNTARFLLG NLHGFDPLQH LVALEDMVLL
710 720 730 740 750
DRWIVHRAHE LQEKIVAAYA RYDFAEIVQA LLNFCSVDLG SLYLDVTKDR
760 770 780 790 800
LYTMAEDARG RRSAQSAMYH VAEAFVRWIA PVMSFTADEL WGYLPGKHVG
810 820 830 840 850
NVLFATWYGG LAPLPADAAL TSADFDKLLA LREQVSKVLE PMRANGAIGA
860 870 880 890 900
ALEAEITVAA DVQTAARWQP LAEELRFLFI SGDVTVTAAS TDDIFVSAQP
910 920 930 940
TTKAKCVRCW HHQSSVGSDP RHPELCSRCV SNIEGPGEER RWF
Length:943
Mass (Da):104,419
Last modified:February 7, 2006 - v1
Checksum:i4D4F90B85AE6A1D4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008229 Genomic DNA. Translation: BAE68267.1.
RefSeqiYP_450541.1. NC_007705.1.

Genome annotation databases

EnsemblBacteriaiBAE68267; BAE68267; BAE68267.
GeneIDi3857879.
KEGGixom:XOO_1512.
PATRICi24112556. VBIXanOry49434_1709.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008229 Genomic DNA. Translation: BAE68267.1 .
RefSeqi YP_450541.1. NC_007705.1.

3D structure databases

ProteinModelPortali Q2P5B0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 342109.XOO_1512.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAE68267 ; BAE68267 ; BAE68267 .
GeneIDi 3857879.
KEGGi xom:XOO_1512.
PATRICi 24112556. VBIXanOry49434_1709.

Phylogenomic databases

eggNOGi COG0060.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci XORY342109:GIX9-1536-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of Xanthomonas oryzae pv. oryzae suggests contribution of large numbers of effector genes and insertion sequences to its race diversity."
    Ochiai H., Inoue Y., Takeya M., Sasaki A., Kaku H.
    Jpn. Agric. Res. Q. 39:275-287(2005)
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MAFF 311018.

Entry informationi

Entry nameiSYI_XANOM
AccessioniPrimary (citable) accession number: Q2P5B0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 7, 2006
Last modified: November 26, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3