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Q2P5B0

- SYI_XANOM

UniProt

Q2P5B0 - SYI_XANOM

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Xanthomonas oryzae pv. oryzae (strain MAFF 311018)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (07 Feb 2006)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei577 – 5771Aminoacyl-adenylateUniRule annotation
    Binding sitei621 – 6211ATPUniRule annotation
    Metal bindingi906 – 9061ZincUniRule annotation
    Metal bindingi909 – 9091ZincUniRule annotation
    Metal bindingi926 – 9261ZincUniRule annotation
    Metal bindingi929 – 9291ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciXORY342109:GIX9-1536-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:XOO1512
    OrganismiXanthomonas oryzae pv. oryzae (strain MAFF 311018)
    Taxonomic identifieri342109 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas
    ProteomesiUP000001957: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 943943Isoleucine--tRNA ligasePRO_1000022142Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi342109.XOO_1512.

    Structurei

    3D structure databases

    ProteinModelPortaliQ2P5B0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi59 – 6911"HIGH" regionAdd
    BLAST
    Motifi618 – 6225"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q2P5B0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTQDYKATLH LPATEFPMRG DLPKREPAML ERWEREGFYA QLRANAAGRP    50
    LFVLHDGPPY ANGQIHLGHA VNKILKDIIV KSKCLAGFDA PYIPGWDCHG 100
    LPIEIAIEKK YGKVGVKLDA AEFRQKCREY ATEQIDLQRR DFKRLGVIGD 150
    WDNPYKTLDF RFEANEIRAL AKVVDNGHLT RGVKPVHWCF DCGSALAEAE 200
    IEYADKVSPT VDIAYPARDP GAVAAAFGAT LPGGVGVAVP IWTTTPWTLP 250
    ASLAVSLGAE LDYVLVEGPA DRGQPRWLVI AEALAAKALA RYGVDEVVVH 300
    GHAKGTALEQ MLLNHPFYAE REIPLLLGDH VSAEDGTGAV HTAPGHGQED 350
    YQVSKQYGLL ERYGAAQINP VDGRGVYLPS TPPLGDTVLA GLHIWKANDV 400
    IIEALHGTGV LLAASKMEHS YPHCWRHKTP IAFRATPQWF ISMEQANLRA 450
    DALKAIESVH WYPSWGQARI AGMVDGRPDW TISRQRTWGV PIALFVHRET 500
    GEPHPRSTEL LRQVADRVEL GGVDVWYTLD AAELLGDEAA DYDKITDILD 550
    VWFDSGVTHE AVLVDRGLPK PADLYLEGSD QHRGWFQSSL LSGVAMDKAA 600
    PYKQCLTHGF TVDEHGRKMS KSLGNGIEPQ DIMKTLGADI LRLWIASADY 650
    SNEMSLSQEI LKRNADAYRR LRNTARFLLG NLHGFDPLQH LVALEDMVLL 700
    DRWIVHRAHE LQEKIVAAYA RYDFAEIVQA LLNFCSVDLG SLYLDVTKDR 750
    LYTMAEDARG RRSAQSAMYH VAEAFVRWIA PVMSFTADEL WGYLPGKHVG 800
    NVLFATWYGG LAPLPADAAL TSADFDKLLA LREQVSKVLE PMRANGAIGA 850
    ALEAEITVAA DVQTAARWQP LAEELRFLFI SGDVTVTAAS TDDIFVSAQP 900
    TTKAKCVRCW HHQSSVGSDP RHPELCSRCV SNIEGPGEER RWF 943
    Length:943
    Mass (Da):104,419
    Last modified:February 7, 2006 - v1
    Checksum:i4D4F90B85AE6A1D4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP008229 Genomic DNA. Translation: BAE68267.1.
    RefSeqiWP_011258399.1. NC_007705.1.
    YP_450541.1. NC_007705.1.

    Genome annotation databases

    EnsemblBacteriaiBAE68267; BAE68267; BAE68267.
    GeneIDi3857879.
    KEGGixom:XOO_1512.
    PATRICi24112556. VBIXanOry49434_1709.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP008229 Genomic DNA. Translation: BAE68267.1 .
    RefSeqi WP_011258399.1. NC_007705.1.
    YP_450541.1. NC_007705.1.

    3D structure databases

    ProteinModelPortali Q2P5B0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 342109.XOO_1512.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAE68267 ; BAE68267 ; BAE68267 .
    GeneIDi 3857879.
    KEGGi xom:XOO_1512.
    PATRICi 24112556. VBIXanOry49434_1709.

    Phylogenomic databases

    eggNOGi COG0060.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci XORY342109:GIX9-1536-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of Xanthomonas oryzae pv. oryzae suggests contribution of large numbers of effector genes and insertion sequences to its race diversity."
      Ochiai H., Inoue Y., Takeya M., Sasaki A., Kaku H.
      Jpn. Agric. Res. Q. 39:275-287(2005)
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MAFF 311018.

    Entry informationi

    Entry nameiSYI_XANOM
    AccessioniPrimary (citable) accession number: Q2P5B0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: February 7, 2006
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3