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Q2P497

- GLND_XANOM

UniProt

Q2P497 - GLND_XANOM

Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Xanthomonas oryzae pv. oryzae (strain MAFF 311018)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 61 (01 Oct 2014)
      Sequence version 1 (07 Feb 2006)
      Previous versions | rss
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    Functioni

    Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

    Catalytic activityi

    UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
    Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

    GO - Molecular functioni

    1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
    2. amino acid binding Source: InterPro
    3. metal ion binding Source: InterPro
    4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. nitrogen compound metabolic process Source: InterPro
    2. regulation of nitrogen utilization Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase, Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    Magnesium

    Enzyme and pathway databases

    BioCyciXORY342109:GIX9-1901-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
    Short name:
    UTase/URUniRule annotation
    Alternative name(s):
    Bifunctional [protein-PII] modification enzymeUniRule annotation
    Bifunctional nitrogen sensor proteinUniRule annotation
    Including the following 2 domains:
    [Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
    Short name:
    PII uridylyltransferaseUniRule annotation
    Short name:
    UTaseUniRule annotation
    [Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
    Short name:
    URUniRule annotation
    Gene namesi
    Name:glnDUniRule annotation
    Ordered Locus Names:XOO1875
    OrganismiXanthomonas oryzae pv. oryzae (strain MAFF 311018)
    Taxonomic identifieri342109 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas
    ProteomesiUP000001957: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 869869Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_0000231698Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi342109.XOO_1875.

    Structurei

    3D structure databases

    ProteinModelPortaliQ2P497.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini451 – 570120HDUniRule annotationAdd
    BLAST
    Domaini692 – 77483ACT 1UniRule annotationAdd
    BLAST
    Domaini798 – 86972ACT 2UniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 332332UridylyltransferaseAdd
    BLAST
    Regioni333 – 691359Uridylyl-removingAdd
    BLAST

    Domaini

    Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

    Sequence similaritiesi

    Belongs to the GlnD family.UniRule annotation
    Contains 2 ACT domains.UniRule annotation
    Contains 1 HD domain.UniRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG2844.
    HOGENOMiHOG000036007.
    KOiK00990.
    OMAiHHLLMSV.
    OrthoDBiEOG6CCH44.

    Family and domain databases

    Gene3Di1.10.3210.10. 1 hit.
    HAMAPiMF_00277. PII_uridylyl_transf.
    InterProiIPR002912. ACT_dom.
    IPR010043. GlnD_Uridyltrans.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR002934. Nucleotidyltransferase.
    IPR013546. PII_UdlTrfase/GS_AdlTrfase.
    [Graphical view]
    PfamiPF01842. ACT. 1 hit.
    PF08335. GlnD_UR_UTase. 1 hit.
    PF01966. HD. 1 hit.
    PF01909. NTP_transf_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
    SMARTiSM00471. HDc. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
    PROSITEiPS51671. ACT. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q2P497-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTDAPAERPD PSVAGDADWA AQARPLLVHA DMRLRKRFDQ GEPIERLVAL    50
    RARAVDQLMR NAWTRCIPAD SGLSLHAVGG YGRGELFPRS DVDVLVLGDS 100
    VAQQRHEQHL SRLFALLWDV GLPISHAVRS PTQCMVAAAD QTVLTALIES 150
    RALVADAAAR AALAAAIAPQ RVWPPRDFFQ AKREELLARH QRFGDTADNL 200
    EPDIKDGPGG LRDLQTLGCM ALRAFGVKDV EALVGLGHVG CDEAAALRRE 250
    REELARLRFG LHIVANRPEE RLRFDYQKTL AERLGFADDL ESLGVEKMMQ 300
    RFYRSAALIR RISDRLLQRF EEQFDGEATP ESLGGGFSLR RGYLAADSDS 350
    WPGDDVLQVF ALFVHWAAHR EVRGLHSLTA RALAEVLREF PAYDVADATA 400
    RELFMALLRG TRAVETLNRM ARLGVLGQWI PAFASVSGRM QFDLFHVYTV 450
    DQHTLMVLRN IALFAAGRAD ERFSIAHEVW PRLRKPELLL LAGLFHDIAK 500
    GRGGDHSELG AVDTRAFCLA HRLSEGDTEL VTWLVEQHLR MSVTAQKQDI 550
    SDPEVIHRFA TLVGTRERLD YLYLLTCADI AGTSPKLWNA WKDRLLADLY 600
    FAARRALREG VEHPPPREER LREARESARA LMQAQGHDDA TIDRQFAGMP 650
    DENFLRFRPE QLAWQAASLI EVEIAQTLVK ARRAVPDNDA LEVFVYSPDR 700
    DGLFAAIVAT LDRKGYGIHR ARVLDAPHDA IFDVFEVLPR DTYADGDPQR 750
    LAATLRQVLA GDLQQVRPAR RAVPGQLRHF RFAPRVEFSE SADGRRTRIS 800
    LVAPDRPGLL ADVAHVLRVQ HLRVHDARIA TFGERAEDQF QITDEHDRPL 850
    SESARQALRD ALCACLDPV 869
    Length:869
    Mass (Da):97,350
    Last modified:February 7, 2006 - v1
    Checksum:iEF45D7310C81BC66
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP008229 Genomic DNA. Translation: BAE68630.1.
    RefSeqiYP_450904.1. NC_007705.1.

    Genome annotation databases

    EnsemblBacteriaiBAE68630; BAE68630; BAE68630.
    GeneIDi3859616.
    KEGGixom:XOO_1875.
    PATRICi24113351. VBIXanOry49434_2104.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP008229 Genomic DNA. Translation: BAE68630.1 .
    RefSeqi YP_450904.1. NC_007705.1.

    3D structure databases

    ProteinModelPortali Q2P497.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 342109.XOO_1875.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAE68630 ; BAE68630 ; BAE68630 .
    GeneIDi 3859616.
    KEGGi xom:XOO_1875.
    PATRICi 24113351. VBIXanOry49434_2104.

    Phylogenomic databases

    eggNOGi COG2844.
    HOGENOMi HOG000036007.
    KOi K00990.
    OMAi HHLLMSV.
    OrthoDBi EOG6CCH44.

    Enzyme and pathway databases

    BioCyci XORY342109:GIX9-1901-MONOMER.

    Family and domain databases

    Gene3Di 1.10.3210.10. 1 hit.
    HAMAPi MF_00277. PII_uridylyl_transf.
    InterProi IPR002912. ACT_dom.
    IPR010043. GlnD_Uridyltrans.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR002934. Nucleotidyltransferase.
    IPR013546. PII_UdlTrfase/GS_AdlTrfase.
    [Graphical view ]
    Pfami PF01842. ACT. 1 hit.
    PF08335. GlnD_UR_UTase. 1 hit.
    PF01966. HD. 1 hit.
    PF01909. NTP_transf_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
    SMARTi SM00471. HDc. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
    PROSITEi PS51671. ACT. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of Xanthomonas oryzae pv. oryzae suggests contribution of large numbers of effector genes and insertion sequences to its race diversity."
      Ochiai H., Inoue Y., Takeya M., Sasaki A., Kaku H.
      Jpn. Agric. Res. Q. 39:275-287(2005)
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MAFF 311018.

    Entry informationi

    Entry nameiGLND_XANOM
    AccessioniPrimary (citable) accession number: Q2P497
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 4, 2006
    Last sequence update: February 7, 2006
    Last modified: October 1, 2014
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3