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Q2P0J4

- TRMB_XANOM

UniProt

Q2P0J4 - TRMB_XANOM

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Protein
tRNA (guanine-N(7)-)-methyltransferase
Gene
trmB, XOO3178
Organism
Xanthomonas oryzae pv. oryzae (strain MAFF 311018)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei75 – 751S-adenosyl-L-methionine By similarity
Binding sitei100 – 1001S-adenosyl-L-methionine By similarity
Binding sitei127 – 1271S-adenosyl-L-methionine By similarity
Active sitei150 – 1501 By similarity
Binding sitei150 – 1501S-adenosyl-L-methionine By similarity
Binding sitei154 – 1541Substrate By similarity
Binding sitei186 – 1861Substrate By similarity

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciXORY342109:GIX9-3230-MONOMER.
    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
    Alternative name(s):
    tRNA (guanine(46)-N(7))-methyltransferase
    tRNA(m7G46)-methyltransferase
    Gene namesi
    Name:trmB
    Ordered Locus Names:XOO3178
    OrganismiXanthomonas oryzae pv. oryzae (strain MAFF 311018)
    Taxonomic identifieri342109 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas
    ProteomesiUP000001957: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 252252tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation
    PRO_0000229212Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi342109.XOO_3178.

    Structurei

    3D structure databases

    ProteinModelPortaliQ2P0J4.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni156 – 1616Interaction with RNA Reviewed prediction
    Regioni223 – 2264Substrate binding Reviewed prediction

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0220.
    KOiK03439.
    OMAiQDNFLCC.
    OrthoDBiEOG6K6VBC.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_01057. tRNA_methyltr_TrmB.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q2P0J4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTDPFTSDGA KMPPKPFTIE AGRRQVRSFV LRQGRFTSAQ QRAFDELWPR    50
    FGLEYLGTPR DLAATFGRDT HKVLEIGFGN GAALRFAAQQ DPSRDYIGIE 100
    VHAPGVGRLL NALEEDGSTH VRLYHHDAVE VLEHEIADGA LDEVRIYFPD 150
    PWHKKRHNKR RLIQPAFAQL LVRKLRDGGR LHAATDWADY AEQMWDVLDA 200
    TPGLVNRAGP RGHVERPAWR PQTHFETRGQ KLGHGVWDLV YDRDPGVGSG 250
    DA 252
    Length:252
    Mass (Da):28,457
    Last modified:February 7, 2006 - v1
    Checksum:i5A3AA0D5B4BEAB19
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP008229 Genomic DNA. Translation: BAE69933.1.
    RefSeqiWP_011259853.1. NC_007705.1.
    YP_452207.1. NC_007705.1.

    Genome annotation databases

    EnsemblBacteriaiBAE69933; BAE69933; BAE69933.
    GeneIDi3856543.
    KEGGixom:XOO_3178.
    PATRICi24116442. VBIXanOry49434_3625.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP008229 Genomic DNA. Translation: BAE69933.1 .
    RefSeqi WP_011259853.1. NC_007705.1.
    YP_452207.1. NC_007705.1.

    3D structure databases

    ProteinModelPortali Q2P0J4.
    ModBasei Search...

    Protein-protein interaction databases

    STRINGi 342109.XOO_3178.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAE69933 ; BAE69933 ; BAE69933 .
    GeneIDi 3856543.
    KEGGi xom:XOO_3178.
    PATRICi 24116442. VBIXanOry49434_3625.

    Phylogenomic databases

    eggNOGi COG0220.
    KOi K03439.
    OMAi QDNFLCC.
    OrthoDBi EOG6K6VBC.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .
    BioCyci XORY342109:GIX9-3230-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_01057. tRNA_methyltr_TrmB.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of Xanthomonas oryzae pv. oryzae suggests contribution of large numbers of effector genes and insertion sequences to its race diversity."
      Ochiai H., Inoue Y., Takeya M., Sasaki A., Kaku H.
      Jpn. Agric. Res. Q. 39:275-287(2005)
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MAFF 311018.

    Entry informationi

    Entry nameiTRMB_XANOM
    AccessioniPrimary (citable) accession number: Q2P0J4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 4, 2006
    Last sequence update: February 7, 2006
    Last modified: September 3, 2014
    This is version 63 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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