Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme

Gene

glnE

Organism
Xanthomonas oryzae pv. oryzae (strain MAFF 311018)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of glutamine synthetase GlnA, a key enzyme in the process to assimilate ammonia. When cellular nitrogen levels are high, the C-terminal adenylyl transferase (AT) inactivates GlnA by covalent transfer of an adenylyl group from ATP to specific tyrosine residue of GlnA, thus reducing its activity. Conversely, when nitrogen levels are low, the N-terminal adenylyl removase (AR) activates GlnA by removing the adenylyl group by phosphorolysis, increasing its activity. The regulatory region of GlnE binds the signal transduction protein PII (GlnB) which indicates the nitrogen status of the cell.UniRule annotation

Catalytic activityi

[Glutamine synthetase]-O4-(5'-adenylyl)-L-tyrosine + phosphate = [glutamine synthetase]-L-tyrosine + ADP.UniRule annotation
ATP + [glutamine synthetase]-L-tyrosine = diphosphate + [glutamine synthetase]-O4-(5'-adenylyl)-L-tyrosine.UniRule annotation

Cofactori

Mg2+UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
LigandATP-binding, Magnesium, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzymeUniRule annotation
Alternative name(s):
ATP:glutamine synthetase adenylyltransferaseUniRule annotation
ATaseUniRule annotation
Including the following 2 domains:
Glutamine synthetase adenylyl-L-tyrosine phosphorylaseUniRule annotation (EC:2.7.7.89UniRule annotation)
Alternative name(s):
Adenylyl removaseUniRule annotation
Short name:
ARUniRule annotation
Short name:
AT-NUniRule annotation
Glutamine synthetase adenylyl transferaseUniRule annotation (EC:2.7.7.42UniRule annotation)
Alternative name(s):
Adenylyl transferaseUniRule annotation
Short name:
ATUniRule annotation
Short name:
AT-CUniRule annotation
Gene namesi
Name:glnEUniRule annotation
Ordered Locus Names:XOO4031
OrganismiXanthomonas oryzae pv. oryzae (strain MAFF 311018)
Taxonomic identifieri342109 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000470191 – 941Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzymeAdd BLAST941

Structurei

3D structure databases

ProteinModelPortaliQ2NY41.
SMRiQ2NY41.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 437Adenylyl removaseUniRule annotationAdd BLAST437
Regioni444 – 941Adenylyl transferaseUniRule annotationAdd BLAST498

Sequence similaritiesi

Belongs to the GlnE family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000060835.
KOiK00982.
OMAiFTVQLLQ.
OrthoDBiPOG091H0675.

Family and domain databases

HAMAPiMF_00802. GlnE. 1 hit.
InterProiView protein in InterPro
IPR023057. GlnE.
IPR005190. GlnE_rpt_dom.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
PfamiView protein in Pfam
PF08335. GlnD_UR_UTase. 2 hits.
PF03710. GlnE. 2 hits.

Sequencei

Sequence statusi: Complete.

Q2NY41-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIPTASLSP ALAALIERAV ARVRQSLPAW QVWPGGDFDR QLAQVALASD
60 70 80 90 100
FALDTLARQP ALLQHLAHPD PPPLPLPQLD PAQPQLWPAQ LRRYRSAEST
110 120 130 140 150
RLVWRDVLGL DSVEATLAGA TRLAEHCMQC GLQALEQQFA TRHGKVIADD
160 170 180 190 200
GSVQRLVVFG LGKLGGGELN FSSDVDLVYA YPQGGQSDGA RSLAAEEYFA
210 220 230 240 250
RLGQQLARLL DEATAEGFSH RVDLRLRPFG TAGRVALSFA GMDQYFQREG
260 270 280 290 300
RDWERYAWLK ARAVAGAIDA GEAWLETLRP FVYRRYLDFT ALDGLREMKA
310 320 330 340 350
AITAEVARHD CLDDIKRGPG GIREIEFLAQ SLQLIRGGRE PSLRERRLLP
360 370 380 390 400
ALRALVTAGQ IDQENGQALT TAYRFLRRLE NRLQMLRDAQ THALPQAPLD
410 420 430 440 450
RERIALGLGY AEWATLLDAL APQRARVTAE FAELLAPRVR ATAPDTLADY
460 470 480 490 500
WRALPAGDAA RLAGMGLSDP GGAHQALADF AHSSGVRGLS DSARARLDRV
510 520 530 540 550
MPALLHAATR ASQPDAAVPR MLGLLQATLR RTSYLSLLDE QPSALARLVD
560 570 580 590 600
VLSRSALLAE RLAAYPLLLD ELLDTRISGP LPDRAALHAA CADIVHIDDT
610 620 630 640 650
EAALRELNER RLARSFRIAL ATLDGRQQAV ESTRQLAWLA EAVVQTVLHL
660 670 680 690 700
ARTEMVAAHG YVSGGSFAII GYGSLGGMEL GFGSDLDLVF LYDHPPEVDA
710 720 730 740 750
SDGKRPLEAG RWFARLAQKV MALLAAETGA GRLYDIDVRL RPDGGKAALV
760 770 780 790 800
SSLASYREYQ RERAWTWEHQ ALVRARAVAG DAVLCDAFAQ VRRYTLMRVR
810 820 830 840 850
DTAQLHEDVR KMRARMRTEL DRSDAGRLDL KQGAGGLVDL EFVLQAGVLG
860 870 880 890 900
LAAQQPQLLD VCDTPALIDA LVQVHWLPDD SAASLHQAHA TLVDAGLSCT
910 920 930 940
LDRRPRLIAP TPAIQQARGT IFNAARVQRL TFPLGKDEAA L
Length:941
Mass (Da):102,911
Last modified:February 7, 2006 - v1
Checksum:iD4421D0377DB2845
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008229 Genomic DNA. Translation: BAE70786.1.
RefSeqiWP_011409641.1. NC_007705.1.

Genome annotation databases

EnsemblBacteriaiBAE70786; BAE70786; BAE70786.
KEGGixom:XOO4031.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiGLNE_XANOM
AccessioniPrimary (citable) accession number: Q2NY41
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 7, 2006
Last modified: June 7, 2017
This is version 73 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families