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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Sodalis glossinidius (strain morsitans)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei561Aminoacyl-adenylateUniRule annotation1
Binding sitei605ATPUniRule annotation1
Metal bindingi901ZincUniRule annotation1
Metal bindingi904ZincUniRule annotation1
Metal bindingi921ZincUniRule annotation1
Metal bindingi924ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:SG0414
OrganismiSodalis glossinidius (strain morsitans)
Taxonomic identifieri343509 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesPectobacteriaceaeSodalis
Proteomesi
  • UP000001932 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000221261 – 938Isoleucine--tRNA ligaseAdd BLAST938

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi343509.SG0414

Structurei

3D structure databases

ProteinModelPortaliQ2NVY6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi58 – 68"HIGH" regionAdd BLAST11
Motifi602 – 606"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q2NVY6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDYKNTLNL PDTGFPMRGD LAKREPGMLE RWYEQDLYGI ICQARKGKKT
60 70 80 90 100
FILHDGPPYA NGSIHIGHSV NKILKDIIIK SKGLMGYDSP YVPGWDCHGL
110 120 130 140 150
PIELKVEQLI GKPGEKVSAA EFRTACRRYA AEQVEGQKKD FIRLGVLGDW
160 170 180 190 200
EHPYLTMNFA TEANIIRALG KIIANGHLHK GAKPVHWCID CRSALAEAEV
210 220 230 240 250
EYYDRTSPSI DVAFAADDVR AVAAKFGAAD FAGQISLIIW TTTPWTLPAN
260 270 280 290 300
RAIAVHPDFD YQLVEVEGQG YILAADLVDS VMARAGITCW TVLGSAKGSA
310 320 330 340 350
LELLRFRHPF MGFDVPAILG QHVTLDAGTG AVHTAPGHGP DDYVIGQQYG
360 370 380 390 400
LEVANPVGPD GCYLPGTLPA LDGLQVFKAN DVVINLLCDS GALLHVEKLQ
410 420 430 440 450
HSYPHCWRHK TSIIFRATPQ WFVSMDQRGL RKQSLAEIKD VQWIPGWGQA
460 470 480 490 500
RIETMVANRP DWCISRQRTW GVPMALFVHN ETEALHPRTI ELMESVAQRV
510 520 530 540 550
EQDGIQAWWD LDPAEILGDD AAHYHKVPDT LDVWFDSGST HSSIVAVRPE
560 570 580 590 600
FEGHAPDMYL EGSDQHRGWF MSSLMISTAM HGKAPYRQVL THGFTVDGQG
610 620 630 640 650
RKMSKSVGNV VSPQQVMDKL GADILRLWVA STDYTGEMAV SDEILKRSAD
660 670 680 690 700
AYRRIRNTAR FLLANLNGFD PARHSVSPEQ MVVLDRWAVG RAQAAQAEIV
710 720 730 740 750
AAYDSYDFHN VVQRMMQFCS VEMGSFYLDI IKDRQYTTKA DSIARRSCQT
760 770 780 790 800
ALYHIIEALV RWMAPIMSFT ADEIWGFMPG ERAQYVFTEE WYDGLFGLDA
810 820 830 840 850
AQPLNDAYWQ ILLQVRSETN KVIEQARADK RIGGSLEARV TLYAEPDLAA
860 870 880 890 900
SLRELGDELY FTLLTSNAVV ADYADAGDDA VQCEGLKGLK IALAKAEGEK
910 920 930
CPRCWHYETD IGQHADHPEI CGRCVTNVAG PGEERKFV
Length:938
Mass (Da):104,502
Last modified:February 7, 2006 - v1
Checksum:i06C0E8D4763ABF4B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008232 Genomic DNA Translation: BAE73689.1
RefSeqiWP_011410277.1, NC_007712.1

Genome annotation databases

EnsemblBacteriaiBAE73689; BAE73689; SG0414
KEGGisgl:SG0414

Similar proteinsi

Entry informationi

Entry nameiSYI_SODGM
AccessioniPrimary (citable) accession number: Q2NVY6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 7, 2006
Last modified: March 28, 2018
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health