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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Sodalis glossinidius (strain morsitans)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei561 – 5611Aminoacyl-adenylateUniRule annotation
Binding sitei605 – 6051ATPUniRule annotation
Metal bindingi901 – 9011ZincUniRule annotation
Metal bindingi904 – 9041ZincUniRule annotation
Metal bindingi921 – 9211ZincUniRule annotation
Metal bindingi924 – 9241ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSGLO343509:GJJC-439-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:SG0414
OrganismiSodalis glossinidius (strain morsitans)
Taxonomic identifieri343509 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSodalis
ProteomesiUP000001932 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 938938Isoleucine--tRNA ligasePRO_1000022126Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi343509.SG0414.

Structurei

3D structure databases

ProteinModelPortaliQ2NVY6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi602 – 6065"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2NVY6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDYKNTLNL PDTGFPMRGD LAKREPGMLE RWYEQDLYGI ICQARKGKKT
60 70 80 90 100
FILHDGPPYA NGSIHIGHSV NKILKDIIIK SKGLMGYDSP YVPGWDCHGL
110 120 130 140 150
PIELKVEQLI GKPGEKVSAA EFRTACRRYA AEQVEGQKKD FIRLGVLGDW
160 170 180 190 200
EHPYLTMNFA TEANIIRALG KIIANGHLHK GAKPVHWCID CRSALAEAEV
210 220 230 240 250
EYYDRTSPSI DVAFAADDVR AVAAKFGAAD FAGQISLIIW TTTPWTLPAN
260 270 280 290 300
RAIAVHPDFD YQLVEVEGQG YILAADLVDS VMARAGITCW TVLGSAKGSA
310 320 330 340 350
LELLRFRHPF MGFDVPAILG QHVTLDAGTG AVHTAPGHGP DDYVIGQQYG
360 370 380 390 400
LEVANPVGPD GCYLPGTLPA LDGLQVFKAN DVVINLLCDS GALLHVEKLQ
410 420 430 440 450
HSYPHCWRHK TSIIFRATPQ WFVSMDQRGL RKQSLAEIKD VQWIPGWGQA
460 470 480 490 500
RIETMVANRP DWCISRQRTW GVPMALFVHN ETEALHPRTI ELMESVAQRV
510 520 530 540 550
EQDGIQAWWD LDPAEILGDD AAHYHKVPDT LDVWFDSGST HSSIVAVRPE
560 570 580 590 600
FEGHAPDMYL EGSDQHRGWF MSSLMISTAM HGKAPYRQVL THGFTVDGQG
610 620 630 640 650
RKMSKSVGNV VSPQQVMDKL GADILRLWVA STDYTGEMAV SDEILKRSAD
660 670 680 690 700
AYRRIRNTAR FLLANLNGFD PARHSVSPEQ MVVLDRWAVG RAQAAQAEIV
710 720 730 740 750
AAYDSYDFHN VVQRMMQFCS VEMGSFYLDI IKDRQYTTKA DSIARRSCQT
760 770 780 790 800
ALYHIIEALV RWMAPIMSFT ADEIWGFMPG ERAQYVFTEE WYDGLFGLDA
810 820 830 840 850
AQPLNDAYWQ ILLQVRSETN KVIEQARADK RIGGSLEARV TLYAEPDLAA
860 870 880 890 900
SLRELGDELY FTLLTSNAVV ADYADAGDDA VQCEGLKGLK IALAKAEGEK
910 920 930
CPRCWHYETD IGQHADHPEI CGRCVTNVAG PGEERKFV
Length:938
Mass (Da):104,502
Last modified:February 7, 2006 - v1
Checksum:i06C0E8D4763ABF4B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008232 Genomic DNA. Translation: BAE73689.1.
RefSeqiWP_011410277.1. NC_007712.1.
YP_454094.1. NC_007712.1.

Genome annotation databases

EnsemblBacteriaiBAE73689; BAE73689; SG0414.
KEGGisgl:SG0414.
PATRICi23647513. VBISodGlo61428_1080.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008232 Genomic DNA. Translation: BAE73689.1.
RefSeqiWP_011410277.1. NC_007712.1.
YP_454094.1. NC_007712.1.

3D structure databases

ProteinModelPortaliQ2NVY6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi343509.SG0414.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAE73689; BAE73689; SG0414.
KEGGisgl:SG0414.
PATRICi23647513. VBISodGlo61428_1080.

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciSGLO343509:GJJC-439-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Massive genome erosion and functional adaptations provide insights into the symbiotic lifestyle of Sodalis glossinidius in the tsetse host."
    Toh H., Weiss B.L., Perkin S.A.H., Yamashita A., Oshima K., Hattori M., Aksoy S.
    Genome Res. 16:149-156(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: morsitans.

Entry informationi

Entry nameiSYI_SODGM
AccessioniPrimary (citable) accession number: Q2NVY6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 7, 2006
Last modified: April 1, 2015
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.