Q2NVY3 (ISPH_SODGM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
EC=1.17.1.2

Gene names

Name:	ispH
Ordered Locus Names:	SG0417

Organism

Sodalis glossinidius (strain morsitans) [Complete proteome] [HAMAP]

Taxonomic identifier

343509 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Sodalis

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity. HAMAP MF_00191
Catalytic activity	Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191 Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191
Cofactor	Binds 1 3Fe-4S cluster per subunit By similarity. HAMAP MF_00191
Pathway	Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP MF_00191 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP MF_00191
Subunit structure	Homodimer By similarity. HAMAP MF_00191
Sequence similarities	Belongs to the IspH family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	3Fe-4S Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: InterPro
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 316	316	4-hydroxy-3-methylbut-2-enyl diphosphate reductase HAMAP MF_00191		PRO_1000021182

Sequences

	Sequence		Length	Mass (Da)
	Q2NVY3 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: D94FA87E9D152775	FASTA	316	34,560
	10 20 30 40 50 60 MRILLANPRG FCAGVDRAIS IVERALEIYG APIYVRHEVV HNRYVVDTLR QRGAIFIEQI 70 80 90 100 110 120 TEVPDGSILI FSAHGVSQAV RAEARSRDLT VFDATCPLVT KVHMEVARAS RKGTEAILIG 130 140 150 160 170 180 HTGHPEVEGT MGQYNNPAGG MYLVESPEDV WQLEVKNVDN LCFMTQTTLS VDDTSDVIDA 190 200 210 220 230 240 LRARFPAIIG PRKDDICYAT TNRQEAVRSL AADADVVLVV GSKNSSNSNR LSELAQRVGK 250 260 270 280 290 300 PAYLIDSAGD IQESWLQGTQ TIGVTAGASA PDILVQQVIE RLRLLGAEGA KELIGREENI 310 VFEVPKELRL EVKTVE

References

[1]

"Massive genome erosion and functional adaptations provide insights into the symbiotic lifestyle of Sodalis glossinidius in the tsetse host." Toh H., Weiss B.L., Perkin S.A.H., Yamashita A., Oshima K., Hattori M., Aksoy S. Genome Res. 16:149-156(2006) [PubMed: 16365377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: morsitans.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP008232 Genomic DNA. Translation: BAE73692.1.
RefSeq	YP_454097.1. NC_007712.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q2NVY3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3867528.
GenomeReviews	Gene locus SG0417 in contig AP008232_GR.
KEGG	sgl:SG0417.
NMPDR	fig|343509.6.peg.977.
PATRIC	23647519. VBISodGlo61428_1083.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0761.
HOGENOM	HBG335228.
OMA	IVHNTYV.
PhylomeDB	Q2NVY3.
ProtClustDB	PRK01045.
Enzyme and pathway databases
BioCyc	SGLO343509:SG0417-MONOMER.
Family and domain databases
HAMAP	MF_00191. IspH. [Tree]
InterPro	IPR003451. LytB. [Graphical view]
KO	K03527.
Pfam	PF02401. LYTB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00216. IspH_lytB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPH_SODGM

Accession

Primary (citable) accession number: Q2NVY3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 7, 2006
Last modified:	January 25, 2012
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program