Reviewed, UniProtKB/Swiss-Prot Q2NVN3 (CYSI_SODGM)

Names and origin

Protein names

Recommended name:
    Sulfite reductase [NADPH] hemoprotein beta-component
      Short name=SIR-HP
      Short name=SIRHP
    EC=1.8.1.2

Gene names

Name:	cysI
Ordered Locus Names:	SG0517

Organism

Sodalis glossinidius (strain morsitans) [Complete proteome] [HAMAP]

Taxonomic identifier

343509 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Sodalis

Protein attributes

Sequence length	566 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This enzyme catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate By similarity.
Catalytic activity	H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH. HAMAP MF_01540
Cofactor	Binds 1 siroheme per subunit By similarity. Binds 1 4Fe-4S cluster per subunit By similarity.
Subunit structure	Alpha(8)-beta4. The alpha component is a flavoprotein, the beta component is a hemoprotein By similarity.
Sequence similarities	Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	4Fe-4S Heme Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation Inferred from electronic annotation. Source: HAMAP
Cellular component	sulfite reductase complex (NADPH) Inferred from electronic annotation. Source: InterPro
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADP or NADPH binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro sulfite reductase (NADPH) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 566	566	Sulfite reductase [NADPH] hemoprotein beta-component HAMAP MF_01540		PRO_0000292964
Sites
	Metal binding	430	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	436	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	475	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	479	1	Iron (siroheme axial ligand) By similarity
	Metal binding	479	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2NVN3-1 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 5EA815C37573BFD5	FASTA	566	63,389
	10 20 30 40 50 60 MSEQQPKAKF SDNERLKGES RFLRGTIAQD LQDGLTGGFN GDNFQLIRFH GMYQQDDRDL 70 80 90 100 110 120 RAERAEEKLE PLINMMLRCR LPGGVITPQQ WLGIDAFAAE QTLYGSIRLT TRQTFQFHGV 130 140 150 160 170 180 LKPKLKGMHQ LLHCLGLDSI ATAGDVNRNV LCTSNPVESV LHRQAWEWAK KISEHLLPKT 190 200 210 220 230 240 RAYAEIWLDG EKTETTDQEP ILGPTYLPRK FKTTVVVPPL NDVDLHANDL NFVAISDNGQ 250 260 270 280 290 300 LVGFNVLVGG GLAMTHGDKS TYPRKASEFG FIALDDTLKI AEAVVTTQRD WGNRSNRKNA 310 320 330 340 350 360 KTKYTLESEG VGAFREEVEV RAGVQFAPIR PYAFTERGDR FGWVKGIDNQ WHLTLFIENG 370 380 390 400 410 420 RILDYPDRTL KTGLAEIARR HKGDFRLTAN QNLIIAGVDK KDKAAIEALA RRHGLINDDA 430 440 450 460 470 480 TPQRKASMAC VAFPTCPLAM AEAERFLPQF VSNVETIMSG HGLAEDEIIL RVTGCSNGCG 490 500 510 520 530 540 RAMLAEIGLV GKAIGRYNLY LGGDRIGTRI PRIYRENITE DEILSIIDDT AGRWARERQP 550 560 QESYGDYLVR AGIVRPVVDS ARDFYD

References

[1]

"Massive genome erosion and functional adaptations provide insights into the symbiotic lifestyle of Sodalis glossinidius in the tsetse host." Toh H., Weiss B.L., Perkin S.A.H., Yamashita A., Oshima K., Hattori M., Aksoy S. Genome Res. 16:149-156(2006) [PubMed: 16365377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP008232 Genomic DNA. Translation: BAE73792.1.
RefSeq	YP_454197.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3868218.
GenomeReviews	Gene locus SG0517 in contig AP008232_GR.
KEGG	sgl:SG0517.
NMPDR	fig|343509.6.peg.1172.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2NVN3.
OMA	Q2NVN3. TRQAFQM.
Enzyme and pathway databases
BioCyc	SGLO343509:SG0517-MON.
Family and domain databases
HAMAP	MF_01540. [Tree]
InterPro	IPR011786. CysI. IPR006066. Nir_Si_BS. IPR006067. Nir_Sir_4Fe4S. IPR005117. NiRdtase/SiRdtase_haem-b_fer. [Graphical view]
Pfam	PF01077. NIR_SIR. 1 hit. PF03460. NIR_SIR_ferr. 2 hits. [Graphical view]
PRINTS	PR00397. SIROHAEM.
TIGRFAMs	TIGR02041. CysI. 1 hit.
PROSITE	PS00365. NIR_SIR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSI_SODGM

Accession

Primary (citable) accession number: Q2NVN3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
Last sequence update:	February 7, 2006
Last modified:	June 16, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)