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Q2NVK9 (GSH1_SODGM) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glutamate--cysteine ligase
EC=6.3.2.2
Alternative name(s):
Gamma-ECS
Short name=GCS
Gamma-glutamylcysteine synthetase
Gene names
Name:gshA
Ordered Locus Names:SG0541
OrganismSodalis glossinidius (strain morsitans) [Complete proteome] [HAMAP]
Taxonomic identifier343509 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Sodalis

Protein attributes

Sequence length520 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine. HAMAP MF_00578

Pathway

Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2. HAMAP MF_00578

Sequence similarities

Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily.

Ontologies

Keywords
   Biological processGlutathione biosynthesis
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processglutathione biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glutamate-cysteine ligase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 520520Glutamate--cysteine ligase HAMAP MF_00578
PRO_1000025191

Sequences

Sequence LengthMass (Da)Tools
Q2NVK9 [UniParc].

Last modified February 7, 2006. Version 1.
Checksum: 455D5E3C0D5167D6

FASTA52058,853
        10         20         30         40         50         60 
MIPDVSTALS WLEANPQALK GIRRGVEREG LRINANGSLA QTPHPESLGS ALTHKWITTD 

        70         80         90        100        110        120 
FAEALLEFIT PVDDDIDHML TLLRDIHRYV ARRLGDERLW PMSMPCFIDS AQPIELAQYG 

       130        140        150        160        170        180 
SSNIGRMKTL YRKGLKNRYS ALMQVIAGVH YNFSLPLAFW QAYAGIRDEA SGKEAISAGY 

       190        200        210        220        230        240 
LRLIRNYYRF GWIILYLFGA SPGICPSFLN GRKTDLLFEQ APSGLIYLPY ATSLRLSDLG 

       250        260        270        280        290        300 
YTNKSQSQLN ITFNHLDEYV RGLKQAIKTP SADYQRMGLQ RGGHYLQLNT NVLQIENELY 

       310        320        330        340        350        360 
APIRPKRVTR DDESPSDALM RGGIEYVEVR SLDINPFSPV GVDEEQARFL DLFLIWCTLA 

       370        380        390        400        410        420 
EAPEMSAEEL RCTRTNWNRV ILEGRKPGLM LGIDCGSTEQ PLTILGKSLF SDLRRVAETL 

       430        440        450        460        470        480 
DSNNGDTHYQ LVCDKLVAGF DNPELTLSAR FMDQLIEHGI GGLGLILAND YRQTLRDEPL 

       490        500        510        520 
QVLNEAQLDE ERLRSWQRQR SLEVADHLSF DEFLASQNGR

« Hide

References

[1]"Massive genome erosion and functional adaptations provide insights into the symbiotic lifestyle of Sodalis glossinidius in the tsetse host."
Toh H., Weiss B.L., Perkin S.A.H., Yamashita A., Oshima K., Hattori M., Aksoy S.
Genome Res. 16:149-156(2006) [PubMed: 16365377] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: morsitans.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AP008232 Genomic DNA. Translation: BAE73816.1.
RefSeqYP_454221.1. NC_007712.1.

3D structure databases

ProteinModelPortalQ2NVK9.
SMRQ2NVK9. Positions 1-514.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ2NVK9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3868657.
GenomeReviewsGene locus SG0541 in contig AP008232_GR.
KEGGsgl:SG0541.
NMPDRfig|343509.6.peg.1228.
PATRIC23648049. VBISodGlo61428_1346.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2918.
HOGENOMHBG289023.
OMAFGLIRNY.
PhylomeDBQ2NVK9.
ProtClustDBPRK02107.

Enzyme and pathway databases

BioCycSGLO343509:SG0541-MONOMER.

Family and domain databases

HAMAPMF_00578. Glu_cys_ligase.
[Tree]
InterProIPR007370. Glu_cys_ligase.
IPR006334. Glut_cys_ligase.
[Graphical view]
KOK01919.
PfamPF04262. Glu_cys_ligase. 1 hit.
[Graphical view]
TIGRFAMsTIGR01434. Glu_cys_ligase. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGSH1_SODGM
AccessionPrimary (citable) accession number: Q2NVK9
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 7, 2006
Last modified: January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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