Reviewed, UniProtKB/Swiss-Prot Q2NVK9 (GSH1_SODGM)

Names and origin

Protein names

Recommended name:
    Glutamate--cysteine ligase
    EC=6.3.2.2
Alternative name(s):
    Gamma-glutamylcysteine synthetase
    Gamma-ECS
      Short name=GCS

Gene names

Name:	gshA
Ordered Locus Names:	SG0541

Organism

Sodalis glossinidius (strain morsitans) [Complete proteome] [HAMAP]

Taxonomic identifier

343509 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Sodalis

Protein attributes

Sequence length	520 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine. HAMAP MF_00578
Pathway	Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2. HAMAP MF_00578
Sequence similarities	Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Glutathione biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutathione biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-cysteine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 520	520	Glutamate--cysteine ligase HAMAP MF_00578		PRO_1000025191

Sequences

	Sequence		Length	Mass (Da)
	Q2NVK9-1 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 455D5E3C0D5167D6	FASTA	520	58,853
	10 20 30 40 50 60 MIPDVSTALS WLEANPQALK GIRRGVEREG LRINANGSLA QTPHPESLGS ALTHKWITTD 70 80 90 100 110 120 FAEALLEFIT PVDDDIDHML TLLRDIHRYV ARRLGDERLW PMSMPCFIDS AQPIELAQYG 130 140 150 160 170 180 SSNIGRMKTL YRKGLKNRYS ALMQVIAGVH YNFSLPLAFW QAYAGIRDEA SGKEAISAGY 190 200 210 220 230 240 LRLIRNYYRF GWIILYLFGA SPGICPSFLN GRKTDLLFEQ APSGLIYLPY ATSLRLSDLG 250 260 270 280 290 300 YTNKSQSQLN ITFNHLDEYV RGLKQAIKTP SADYQRMGLQ RGGHYLQLNT NVLQIENELY 310 320 330 340 350 360 APIRPKRVTR DDESPSDALM RGGIEYVEVR SLDINPFSPV GVDEEQARFL DLFLIWCTLA 370 380 390 400 410 420 EAPEMSAEEL RCTRTNWNRV ILEGRKPGLM LGIDCGSTEQ PLTILGKSLF SDLRRVAETL 430 440 450 460 470 480 DSNNGDTHYQ LVCDKLVAGF DNPELTLSAR FMDQLIEHGI GGLGLILAND YRQTLRDEPL 490 500 510 520 QVLNEAQLDE ERLRSWQRQR SLEVADHLSF DEFLASQNGR

References

[1]

"Massive genome erosion and functional adaptations provide insights into the symbiotic lifestyle of Sodalis glossinidius in the tsetse host." Toh H., Weiss B.L., Perkin S.A.H., Yamashita A., Oshima K., Hattori M., Aksoy S. Genome Res. 16:149-156(2006) [PubMed: 16365377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP008232 Genomic DNA. Translation: BAE73816.1.
RefSeq	YP_454221.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3868657.
GenomeReviews	Gene locus SG0541 in contig AP008232_GR.
KEGG	sgl:SG0541.
NMPDR	fig|343509.6.peg.1228.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2NVK9.
OMA	Q2NVK9. EYIEVRA.
Enzyme and pathway databases
BioCyc	SGLO343509:SG0541-MON.
Family and domain databases
HAMAP	MF_00578. [Tree]
InterPro	IPR007370. Glu_cys_ligase. IPR006334. Glut_cys_ligase. [Graphical view]
Pfam	PF04262. Glu_cys_ligase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01434. glu_cys_ligase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GSH1_SODGM

Accession

Primary (citable) accession number: Q2NVK9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 7, 2006
Last modified:	June 16, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)