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Q2NUJ7 (BIOB_SODGM) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:SG0903
OrganismSodalis glossinidius (strain morsitans) [Complete proteome] [HAMAP]
Taxonomic identifier343509 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSodalis

Protein attributes

Sequence length345 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 345345Biotin synthase HAMAP-Rule MF_01694
PRO_0000381638

Sites

Metal binding531Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding571Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding601Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding971Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1281Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1881Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2601Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2NUJ7 [UniParc].

Last modified February 7, 2006. Version 1.
Checksum: B73564C6BA4A3173

FASTA34538,359
        10         20         30         40         50         60 
MSTSQRWTQA QARALFDKPF LELLFEAQQV HRQHFTPGEV QVSTLLSIKT GACPEDCKYC 

        70         80         90        100        110        120 
PQSARYSTGL ESERLMQVQQ VLDAARKARD AGSTRFCMGA AWKNPHDRDM PLLEQMVQGV 

       130        140        150        160        170        180 
KAMGLETCMT LGMLNDRQVQ RLAEAGLDFY NHNLDTSPEF YGSIVTTRSY QDRLDTLSKV 

       190        200        210        220        230        240 
RQAGIKVCSG GIVGLGEDIR DRAGLLVQLA NLPTPPESVP INMLVKVKGT PLADNEDVDP 

       250        260        270        280        290        300 
FEFIRTIAVA RIMMPRSHVR LSAGREQMNE QTQALCFMAG ANSVFYGCKL LTTPNPGEDR 

       310        320        330        340 
DMALFHKLGI NIERRTATQG DVAQQAQLAE ELLTADTAHY YNAAR 

« Hide

References

[1]"Massive genome erosion and functional adaptations provide insights into the symbiotic lifestyle of Sodalis glossinidius in the tsetse host."
Toh H., Weiss B.L., Perkin S.A.H., Yamashita A., Oshima K., Hattori M., Aksoy S.
Genome Res. 16:149-156(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: morsitans.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP008232 Genomic DNA. Translation: BAE74178.1.
RefSeqYP_454583.1. NC_007712.1.

3D structure databases

ProteinModelPortalQ2NUJ7.
SMRQ2NUJ7. Positions 6-314.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING343509.SG0903.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAE74178; BAE74178; SG0903.
GeneID3868351.
KEGGsgl:SG0903.
PATRIC23649480. VBISodGlo61428_2048.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
KOK01012.
OMARIMMPAS.
OrthoDBEOG622PMP.

Enzyme and pathway databases

BioCycSGLO343509:GJJC-945-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_SODGM
AccessionPrimary (citable) accession number: Q2NUJ7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 7, 2006
Last modified: May 14, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways