Reviewed, UniProtKB/Swiss-Prot Q2NUD3 (MAO1_SODGM)

Names and origin

Protein names

Recommended name:
    NAD-dependent malic enzyme
      Short name=NAD-ME
    EC=1.1.1.38

Gene names

Name:	sfcA
Ordered Locus Names:	SG0967

Organism

Sodalis glossinidius (strain morsitans) [Complete proteome] [HAMAP]

Taxonomic identifier

343509 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Sodalis

Protein attributes

Sequence length	565 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate + NAD(+) = pyruvate + CO(2) + NADH.
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the malic enzymes family.

Ontologies

Keywords
Ligand	Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 565	565	NAD-dependent malic enzyme		PRO_1000069550
Sites
	Active site	104	1	Proton donor By similarity
	Active site	175	1	Proton acceptor By similarity
	Metal binding	246	1	Divalent metal cation By similarity
	Metal binding	247	1	Divalent metal cation By similarity
	Metal binding	270	1	Divalent metal cation By similarity
	Binding site	157	1	NAD By similarity
	Binding site	270	1	NAD By similarity
	Binding site	418	1	NAD By similarity
	Site	270	1	Important for activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2NUD3-1 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 54D4A810844D312E	FASTA	565	62,560
	10 20 30 40 50 60 MELEYESKRP LYIPHAGPIL LEFPLLNKGS AFSLEERDNF NLQGLLPDTV ETIEEQAERA 70 80 90 100 110 120 WRQYQDFRTN IDRHIYLRNI QDTNETLFYR LLAAHLAEML PIIYTPTVGD ACERFSDIYR 130 140 150 160 170 180 RARGVFISYN NCDKIEDMLQ NATKQNVKVI VVTDGERILG LGDQGIGGMG IPIGKLSLYT 190 200 210 220 230 240 ACGGISPAYT LPVVLDAGTN NQQLLNDPLY MGWRHPRITG EQYDKFVDAF IQAVKRRWPN 250 260 270 280 290 300 VLLQFEDFAQ KNATPLLNRY RSELCCFNDD IQGTAAVTLG CLLAASRAAG KRLRDQKVVF 310 320 330 340 350 360 LGAGSAGCGI AEQITAEMRT EGLSEDEARR RVLMVDRFGL LTDKLANLLD FQSRLVQPSD 370 380 390 400 410 420 SLSDWQLDSD TISLQDVVRN ARPTVLIGVS GQPGLFTEEI IRDMHQHCER PIVMPLSNPT 430 440 450 460 470 480 SRVEATPEDL LRWTDGAALV ATGSPFAPVQ FEGKTYPIAQ CNNAYIFPGI GLGVLASGAG 490 500 510 520 530 540 QITDAMLIAA SRALADCSPL ANGDGGALLP DINDIQSVSR VIAMAVAKAA QVHGVALVTS 550 560 EETLSLAIEH NFWQPQYRDY RRTSF

References

[1]

"Massive genome erosion and functional adaptations provide insights into the symbiotic lifestyle of Sodalis glossinidius in the tsetse host." Toh H., Weiss B.L., Perkin S.A.H., Yamashita A., Oshima K., Hattori M., Aksoy S. Genome Res. 16:149-156(2006) [PubMed: 16365377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP008232 Genomic DNA. Translation: BAE74242.1.
RefSeq	YP_454647.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3867094.
GenomeReviews	Gene locus SG0967 in contig AP008232_GR.
KEGG	sgl:SG0967.
NMPDR	fig|343509.6.peg.2010.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2NUD3.
Enzyme and pathway databases
BioCyc	SGLO343509:SG0967-MON.
Family and domain databases
HAMAP	MF_01619. [Tree]
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD_bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view]
PRINTS	PR00072. MALOXRDTASE.
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAO1_SODGM

Accession

Primary (citable) accession number: Q2NUD3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	February 7, 2006
Last modified:	November 25, 2008
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)