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Protein

Alpha,alpha-trehalose-phosphate synthase [UDP-forming]

Gene

otsA

Organism
Sodalis glossinidius (strain morsitans)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor (By similarity).By similarity

Catalytic activityi

UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei10 – 101Glucose-6-phosphateBy similarity
Binding sitei77 – 771Glucose-6-phosphateBy similarity
Sitei86 – 861Involved in alpha anomer selectivityBy similarity
Binding sitei131 – 1311Glucose-6-phosphateBy similarity
Sitei156 – 1561Involved in alpha anomer selectivityBy similarity
Binding sitei262 – 2621UDP-glucoseBy similarity
Binding sitei267 – 2671UDP-glucoseBy similarity
Binding sitei300 – 3001Glucose-6-phosphateBy similarity
Binding sitei339 – 3391UDP-glucose; via amide nitrogen and carbonyl oxygenBy similarity

GO - Molecular functioni

  1. alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity Source: UniProtKB-EC

GO - Biological processi

  1. trehalose biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciSGLO343509:GJJC-1291-MONOMER.
UniPathwayiUPA00299.

Protein family/group databases

CAZyiGT20. Glycosyltransferase Family 20.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha,alpha-trehalose-phosphate synthase [UDP-forming] (EC:2.4.1.15)
Alternative name(s):
Osmoregulatory trehalose synthesis protein A
Trehalose-6-phosphate synthase
UDP-glucose-glucosephosphate glucosyltransferase
Gene namesi
Name:otsA
Ordered Locus Names:SG1241
OrganismiSodalis glossinidius (strain morsitans)
Taxonomic identifieri343509 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSodalis
ProteomesiUP000001932 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 469469Alpha,alpha-trehalose-phosphate synthase [UDP-forming]PRO_0000348921Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi343509.SG1241.

Structurei

3D structure databases

SMRiQ2NTK9. Positions 2-453.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni22 – 232UDP-glucose bindingBy similarity
Regioni365 – 3695UDP-glucose bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyltransferase 20 family.Curated

Phylogenomic databases

eggNOGiCOG0380.
KOiK00697.
OMAiIEFMPIH.
OrthoDBiEOG6CCH1M.

Family and domain databases

InterProiIPR001830. Glyco_trans_20.
IPR012766. Trehalose_OtsA.
[Graphical view]
PfamiPF00982. Glyco_transf_20. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02400. trehalose_OtsA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2NTK9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRLVVVSNR IAAIEGKKES AGGLAVGIMD SLKDQGGLWF GWNGKISEED
60 70 80 90 100
EPLEKNQQDN ITFAAFSLKQ SEYDQYYLNF SNTVIWPAFH YRLDLVQYQR
110 120 130 140 150
EDYDGYCRVN EMLAGRLKPL VNEDDILWIH DYHLLPFAAA CRKLGMKNRI
160 170 180 190 200
GFFLHIPFPT SEIFNALPPR KELLEKLCEY DLVGFQAESD RQAFIENLAL
210 220 230 240 250
VTTVEDLDDD RIKAYNKLVT ARVYPIGVEP ESIRELAEGP LPPKLAHLRD
260 270 280 290 300
KMNGQLIISV DRLDYSKGLP ERFQAYETLL ENYPQHRGNI RYFQIAPTSR
310 320 330 340 350
GDVQAYQDIR HELETEAGRI NGHFSTLEWT PLFYLNQHYE RSLLMKIFRH
360 370 380 390 400
CEVGLVTPLR DGMNLVAKEY VASQNPNDPG VLILSHFAGA ANELTSALLV
410 420 430 440 450
NPYDRDGVAS ALDKALSMPL SERKARYQEM IAVIKQNDIV HWCQSFLDDL
460
KKIPSKAEIV GQAVSGATR
Length:469
Mass (Da):53,446
Last modified:February 7, 2006 - v1
Checksum:i9E96324BCDDFDFF6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008232 Genomic DNA. Translation: BAE74516.1.
RefSeqiYP_454921.1. NC_007712.1.

Genome annotation databases

EnsemblBacteriaiBAE74516; BAE74516; SG1241.
KEGGisgl:SG1241.
PATRICi23651168. VBISodGlo61428_2884.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008232 Genomic DNA. Translation: BAE74516.1.
RefSeqiYP_454921.1. NC_007712.1.

3D structure databases

SMRiQ2NTK9. Positions 2-453.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi343509.SG1241.

Protein family/group databases

CAZyiGT20. Glycosyltransferase Family 20.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAE74516; BAE74516; SG1241.
KEGGisgl:SG1241.
PATRICi23651168. VBISodGlo61428_2884.

Phylogenomic databases

eggNOGiCOG0380.
KOiK00697.
OMAiIEFMPIH.
OrthoDBiEOG6CCH1M.

Enzyme and pathway databases

UniPathwayiUPA00299.
BioCyciSGLO343509:GJJC-1291-MONOMER.

Family and domain databases

InterProiIPR001830. Glyco_trans_20.
IPR012766. Trehalose_OtsA.
[Graphical view]
PfamiPF00982. Glyco_transf_20. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02400. trehalose_OtsA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Massive genome erosion and functional adaptations provide insights into the symbiotic lifestyle of Sodalis glossinidius in the tsetse host."
    Toh H., Weiss B.L., Perkin S.A.H., Yamashita A., Oshima K., Hattori M., Aksoy S.
    Genome Res. 16:149-156(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: morsitans.

Entry informationi

Entry nameiOTSA_SODGM
AccessioniPrimary (citable) accession number: Q2NTK9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: February 7, 2006
Last modified: April 1, 2015
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.