Q2NTK9 (OTSA_SODGM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha,alpha-trehalose-phosphate synthase [UDP-forming]
EC=2.4.1.15

Alternative name(s):
Osmoregulatory trehalose synthesis protein A
Trehalose-6-phosphate synthase
UDP-glucose-glucosephosphate glucosyltransferase

Gene names

Name:	otsA
Ordered Locus Names:	SG1241

Organism

Sodalis glossinidius (strain morsitans) [Complete proteome] [HAMAP]

Taxonomic identifier

343509 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Sodalis › Sodalis glossinidius

Protein attributes

Sequence length	469 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor By similarity.
Catalytic activity	UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.
Pathway	Glycan biosynthesis; trehalose biosynthesis.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the glycosyltransferase 20 family.

Ontologies

Keywords
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	trehalose biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 469	469	Alpha,alpha-trehalose-phosphate synthase [UDP-forming]		PRO_0000348921
Regions
	Region	22 – 23	2	UDP-glucose binding By similarity
	Region	365 – 369	5	UDP-glucose binding By similarity
Sites
	Binding site	10	1	Glucose-6-phosphate By similarity
	Binding site	77	1	Glucose-6-phosphate By similarity
	Binding site	131	1	Glucose-6-phosphate By similarity
	Binding site	262	1	UDP-glucose By similarity
	Binding site	267	1	UDP-glucose By similarity
	Binding site	300	1	Glucose-6-phosphate By similarity
	Binding site	339	1	UDP-glucose; via amide nitrogen and carbonyl oxygen By similarity
	Site	86	1	Involved in alpha anomer selectivity By similarity
	Site	156	1	Involved in alpha anomer selectivity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2NTK9 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 9E96324BCDDFDFF6	FASTA	469	53,446
	10 20 30 40 50 60 MSRLVVVSNR IAAIEGKKES AGGLAVGIMD SLKDQGGLWF GWNGKISEED EPLEKNQQDN 70 80 90 100 110 120 ITFAAFSLKQ SEYDQYYLNF SNTVIWPAFH YRLDLVQYQR EDYDGYCRVN EMLAGRLKPL 130 140 150 160 170 180 VNEDDILWIH DYHLLPFAAA CRKLGMKNRI GFFLHIPFPT SEIFNALPPR KELLEKLCEY 190 200 210 220 230 240 DLVGFQAESD RQAFIENLAL VTTVEDLDDD RIKAYNKLVT ARVYPIGVEP ESIRELAEGP 250 260 270 280 290 300 LPPKLAHLRD KMNGQLIISV DRLDYSKGLP ERFQAYETLL ENYPQHRGNI RYFQIAPTSR 310 320 330 340 350 360 GDVQAYQDIR HELETEAGRI NGHFSTLEWT PLFYLNQHYE RSLLMKIFRH CEVGLVTPLR 370 380 390 400 410 420 DGMNLVAKEY VASQNPNDPG VLILSHFAGA ANELTSALLV NPYDRDGVAS ALDKALSMPL 430 440 450 460 SERKARYQEM IAVIKQNDIV HWCQSFLDDL KKIPSKAEIV GQAVSGATR

References

[1]

"Massive genome erosion and functional adaptations provide insights into the symbiotic lifestyle of Sodalis glossinidius in the tsetse host." Toh H., Weiss B.L., Perkin S.A.H., Yamashita A., Oshima K., Hattori M., Aksoy S. Genome Res. 16:149-156(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: morsitans.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP008232 Genomic DNA. Translation: BAE74516.1.
RefSeq	YP_454921.1. NC_007712.1.
3D structure databases
ProteinModelPortal	Q2NTK9.
SMR	Q2NTK9. Positions 2-453.
ModBase	Search...
Protein-protein interaction databases
STRING	343509.SG1241.
Protein family/group databases
CAZy	GT20. Glycosyltransferase Family 20.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAE74516; BAE74516; ENAG00097716.
GeneID	3866764.
KEGG	sgl:SG1241.
PATRIC	23651168. VBISodGlo61428_2884.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0380.
KO	K00697.
OMA	EYGRKEM.
ProtClustDB	PRK10117.
Enzyme and pathway databases
BioCyc	SGLO343509:GJJC-1291-MONOMER.
UniPathway	UPA00299.
Family and domain databases
InterPro	IPR001830. Glyco_trans_20. IPR012766. Trehalose_OtsA. [Graphical view]
Pfam	PF00982. Glyco_transf_20. 1 hit. [Graphical view]
TIGRFAMs	TIGR02400. trehalose_OtsA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

OTSA_SODGM

Accession

Primary (citable) accession number: Q2NTK9

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	February 7, 2006
Last modified:	May 1, 2013
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program