Reviewed, UniProtKB/Swiss-Prot Q2NS22 (PUR4_SODGM)

Names and origin

Protein names

Recommended name:
    Phosphoribosylformylglycinamidine synthase
      Short name=FGAM synthase
      Short name=FGAMS
    EC=6.3.5.3
Alternative name(s):
    Formylglycinamide ribotide amidotransferase
      Short name=FGARAT
    Formylglycinamide ribotide synthetase

Gene names

Name:	purL
Ordered Locus Names:	SG1778

Organism

Sodalis glossinidius (strain morsitans) [Complete proteome] [HAMAP]

Taxonomic identifier

343509 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Sodalis

Protein attributes

Sequence length	1295 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00419
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00419
Subunit structure	Monomer. HAMAP MF_00419
Subcellular location	Cytoplasm. HAMAP MF_00419
Sequence similarities	In the N-terminal section; belongs to the FGAMS family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1295	1295	Phosphoribosylformylglycinamidine synthase HAMAP MF_00419		PRO_0000264600
Regions
	Domain	1042 – 1295	254	Glutamine amidotransferase type-1
	Nucleotide binding	306 – 317	12	ATP Potential
Sites
	Active site	1135	1	Nucleophile By similarity
	Active site	1260	1	By similarity
	Active site	1262	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2NS22-1 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 9A0E14C09C612DF8	FASTA	1,295	141,057
	10 20 30 40 50 60 MEILRGSPAL SAFRINKLLV RCRDARLAVD DIYAEYIHFA DVSAALDEDA LSRLQRLLKY 70 80 90 100 110 120 GPSLAEHQPQ GRLLLVTPRP GTRSPWSSKA TDIAHNCGLP QIKRLERGLA YYIQAPQLSE 130 140 150 160 170 180 PQWGYLAALL HDRMMETVFT RLEEAAALFA QHAPAPVTLV DVLGEGRGAL EAANLALGLA 190 200 210 220 230 240 LAQDEIDYLF AAFTRLGRNP SDVELYMFAQ ANSEHCRHKI FNADWVIDGQ PQAKSLFKMI 250 260 270 280 290 300 KNTFEQTPEY VLSAYKDNAS VMEGSAVGRF FPDAQAGRYD YHQEATHILM KVETHNHPTA 310 320 330 340 350 360 ISPWPGAATG SGGEIRDEGA TGRGAKPKAG LVGFSVSNLR IPGFEQPWEE DFGRPERIVS 370 380 390 400 410 420 ALDIMTDGPL GGAAFNNEFG RPALTGYFRT YEERVDSHNG VELRGYHKPV MLAGGIGNIR 430 440 450 460 470 480 ASHVQKGEIS VGAKLIVLGG PAMNIGLGGG AASSMASGQS DADLDFASVQ RDNPEMERRC 490 500 510 520 530 540 QEVIDRCWQR GEENPILFIH DVGAGGLSNA MPELVSDGGR GGRFQLREIP NDEPGMSPLE 550 560 570 580 590 600 VWCNESQERY VMAVAPERLA EFDAICRREC APYAVIGEAT DALHLSLDDA HYDNRPIDLP 610 620 630 640 650 660 LDVLLGKTPK MQREAVSLQA AGFPLNRDGI KLAEAINRVL HLPAVAEKTF LITIGDRSVT 670 680 690 700 710 720 GMVARDQMVG PWQVPVADCA VTTASLDSYY GEAMSLGERA PVALLNFAAS ARLAVGEALT 730 740 750 760 770 780 NLAATHIGDI KRVKLSANWM AAAGHPGEDA GLYQAVKAVG EELCPALGLT IPVGKDSMSM 790 800 810 820 830 840 KTRWQHGAES REMTAPLSLV ITAFARVEDV RATVTPQLQP ARDNVLLLID LGAGHHALGA 850 860 870 880 890 900 TALAQVYRQL GDETSDVRDA GQLAAFFRVM QQLVAQGWLL AYHDRADGGL LVTLAEMAFA 910 920 930 940 950 960 GHCGIDADIG PLGDDALAAL FNEELGAVIQ IDEADRDAIT ALFHQEGLAD CLHYLGTAQP 970 980 990 1000 1010 1020 GDRFILRAGE RSLYSESRTT LRTWWAETSW QMQRLRDNPE SADQEHASRQ DDNDPGLTVA 1030 1040 1050 1060 1070 1080 LSFDPKDDIA APFIAKGARP KVAVLREQGV NSHVEMAAAF HRAGFEAIDV HMSDLFTGVQ 1090 1100 1110 1120 1130 1140 TLEGFHTLVA CGGFSYGDVL GAGEGWAKSV LFNLRVRDEF EAFFHRPQTL ALGVCNGCQM 1150 1160 1170 1180 1190 1200 MSNLRELIPG AELWPRFVRN KSERFEARFS LVEVTQSQSL LLQGMVGSRL PIAVSHGEGR 1210 1220 1230 1240 1250 1260 VEVRDAAHLA AIEHAGLVAL RYVDNYGKVT ENYPANPNGS PNGITAVTNA SGRVTLTMPH 1270 1280 1290 PERVFRTVSH SWHPAEWGED GPWMRLFRNA RKQLG

References

[1]

"Massive genome erosion and functional adaptations provide insights into the symbiotic lifestyle of Sodalis glossinidius in the tsetse host." Toh H., Weiss B.L., Perkin S.A.H., Yamashita A., Oshima K., Hattori M., Aksoy S. Genome Res. 16:149-156(2006) [PubMed: 16365377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP008232 Genomic DNA. Translation: BAE75053.1.
RefSeq	YP_455458.1.
3D structure databases
SMR	Q2NS22. Positions 1-1295.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2NS22.
Genome annotation databases
GeneID	3866522.
GenomeReviews	Gene locus SG1778 in contig AP008232_GR.
KEGG	sgl:SG1778.
NMPDR	fig|343509.6.peg.3791.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2NS22.
OMA	ERGIAYY.
Enzyme and pathway databases
BioCyc	SGLO343509:SG1778-MON.
Family and domain databases
HAMAP	MF_00419. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR017926. GATASE_1. IPR010073. PRibForGlyAmidine_synth. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 2 hits. [Graphical view]
TIGRFAMs	TIGR01735. FGAM_synt. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR4_SODGM

Accession

Primary (citable) accession number: Q2NS22

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	February 7, 2006
Last modified:	November 3, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)