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Q2NRK5

- GLND_SODGM

UniProt

Q2NRK5 - GLND_SODGM

Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Sodalis glossinidius (strain morsitans)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 1 (07 Feb 2006)
      Previous versions | rss
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    Functioni

    Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

    Catalytic activityi

    UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
    Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

    GO - Molecular functioni

    1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
    2. amino acid binding Source: InterPro
    3. metal ion binding Source: InterPro
    4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. nitrogen compound metabolic process Source: InterPro
    2. regulation of nitrogen utilization Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase, Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    Magnesium

    Enzyme and pathway databases

    BioCyciSGLO343509:GJJC-2011-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
    Short name:
    UTase/URUniRule annotation
    Alternative name(s):
    Bifunctional [protein-PII] modification enzymeUniRule annotation
    Bifunctional nitrogen sensor proteinUniRule annotation
    Including the following 2 domains:
    [Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
    Short name:
    PII uridylyltransferaseUniRule annotation
    Short name:
    UTaseUniRule annotation
    [Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
    Short name:
    URUniRule annotation
    Gene namesi
    Name:glnDUniRule annotation
    Ordered Locus Names:SG1945
    OrganismiSodalis glossinidius (strain morsitans)
    Taxonomic identifieri343509 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSodalis
    ProteomesiUP000001932: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 896896Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_1000204801Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi343509.SG1945.

    Structurei

    3D structure databases

    ProteinModelPortaliQ2NRK5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini474 – 607134HDUniRule annotationAdd
    BLAST
    Domaini715 – 79682ACT 1UniRule annotationAdd
    BLAST
    Domaini822 – 89675ACT 2UniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 355355UridylyltransferaseAdd
    BLAST
    Regioni356 – 714359Uridylyl-removingAdd
    BLAST

    Domaini

    Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

    Sequence similaritiesi

    Belongs to the GlnD family.UniRule annotation
    Contains 2 ACT domains.UniRule annotation
    Contains 1 HD domain.UniRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG2844.
    KOiK00990.
    OMAiHHLLMSV.
    OrthoDBiEOG6CCH44.

    Family and domain databases

    Gene3Di1.10.3210.10. 1 hit.
    HAMAPiMF_00277. PII_uridylyl_transf.
    InterProiIPR002912. ACT_dom.
    IPR010043. GlnD_Uridyltrans.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR002934. Nucleotidyltransferase.
    IPR013546. PII_UdlTrfase/GS_AdlTrfase.
    [Graphical view]
    PfamiPF01842. ACT. 2 hits.
    PF08335. GlnD_UR_UTase. 1 hit.
    PF01966. HD. 1 hit.
    PF01909. NTP_transf_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
    SMARTiSM00471. HDc. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
    PROSITEiPS51671. ACT. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q2NRK5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQGDPMPDQI LQFTHPPVPR QPPVKPSSPL TWPDDALNRP FLKERLDEFQ    50
    RWLADAFDAG ETAEILVDAR TLYIDQLLRQ LWHYHGLAHA PHTALVAVGG 100
    YGRGELHPLS DIDVLVLSQA PLTPPQQQHI SELLTLLWDL KLEVGHSVRS 150
    IAECLEEGRA DLTVATNLIE SRLICGDLAL FLTLQKQVFS DDFWPSRAFF 200
    PAKLAEQQER HSRYHGTSYN LEPDIKSSPG GLRDIHTLLW VAHRHFGATS 250
    MDEMVGFGFI TRAERDELNE CQSFLWRTRF ALHLVLNRYD NRLLFDRQQN 300
    VAQLLRYQGE GNLPVERMMK DFFRVTRRVS ELNQMLLQLF DEAILALAPD 350
    EKPQPLDDEF QLRGNLIDLR DETLFSRQPE AILLLFWQMV RNRHIEGIYS 400
    ATLRQLRYAR RHLKAPLCTS PEARTLFMRI LHQPGAVKHA LLPMHRHSVL 450
    WAYMPQWSNI VGQMQFDLFH AYTVDEHTIR VLLKLESFAD ESQRPYHPLC 500
    VELFPRLSQR HLLLVAALFH DIAKGRGGDH SQLGARDVLE FAALHDLPAE 550
    DAQLVAWLVE SHLVMSVTAQ RRDIQEPDVL VQFAGEMQSE SRLHYLLCLT 600
    VADICATNET LWNSWKQSLL RELFFATEKQ LRRGIHVSPD VRERVRHNRR 650
    QSLVLLRMEG IDEQRLQEIW SRCRADYFLR HTPNQLAWHA RHMVLHDHDE 700
    PLVLISPQAT RGGTEIFIHN QDRPYLFAAV TGELDRRNLS VHDAQIFTNR 750
    DGMAMDTFVV LEPDGSPLSP DRHPVIRQAL LQILLPGDYR HPRVRRPSSK 800
    LRHFNVDTSV VFLPSPTERR SYLELTALDQ PGLLARVSEV FVDLGISLHG 850
    ARISTIGERV EDLFILADGD RRALSRAMQA EVRRRLTEAL NPNDKV 896
    Length:896
    Mass (Da):103,328
    Last modified:February 7, 2006 - v1
    Checksum:i2BAC301F1C44FF4D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP008232 Genomic DNA. Translation: BAE75220.1.
    RefSeqiYP_455625.1. NC_007712.1.

    Genome annotation databases

    EnsemblBacteriaiBAE75220; BAE75220; SG1945.
    GeneIDi3867857.
    KEGGisgl:SG1945.
    PATRICi23654843. VBISodGlo61428_4706.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP008232 Genomic DNA. Translation: BAE75220.1 .
    RefSeqi YP_455625.1. NC_007712.1.

    3D structure databases

    ProteinModelPortali Q2NRK5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 343509.SG1945.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAE75220 ; BAE75220 ; SG1945 .
    GeneIDi 3867857.
    KEGGi sgl:SG1945.
    PATRICi 23654843. VBISodGlo61428_4706.

    Phylogenomic databases

    eggNOGi COG2844.
    KOi K00990.
    OMAi HHLLMSV.
    OrthoDBi EOG6CCH44.

    Enzyme and pathway databases

    BioCyci SGLO343509:GJJC-2011-MONOMER.

    Family and domain databases

    Gene3Di 1.10.3210.10. 1 hit.
    HAMAPi MF_00277. PII_uridylyl_transf.
    InterProi IPR002912. ACT_dom.
    IPR010043. GlnD_Uridyltrans.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR002934. Nucleotidyltransferase.
    IPR013546. PII_UdlTrfase/GS_AdlTrfase.
    [Graphical view ]
    Pfami PF01842. ACT. 2 hits.
    PF08335. GlnD_UR_UTase. 1 hit.
    PF01966. HD. 1 hit.
    PF01909. NTP_transf_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
    SMARTi SM00471. HDc. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
    PROSITEi PS51671. ACT. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Massive genome erosion and functional adaptations provide insights into the symbiotic lifestyle of Sodalis glossinidius in the tsetse host."
      Toh H., Weiss B.L., Perkin S.A.H., Yamashita A., Oshima K., Hattori M., Aksoy S.
      Genome Res. 16:149-156(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: morsitans.

    Entry informationi

    Entry nameiGLND_SODGM
    AccessioniPrimary (citable) accession number: Q2NRK5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: February 7, 2006
    Last modified: October 1, 2014
    This is version 70 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3