Q2NRK0 (QUEF_SODGM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductase
EC=1.7.1.13

Alternative name(s):
7-cyano-7-carbaguanine reductase
NADPH-dependent nitrile oxidoreductase
PreQ(0) reductase

Gene names

Name:	queF
Ordered Locus Names:	SG1950

Organism

Sodalis glossinidius (strain morsitans) [Complete proteome] [HAMAP]

Taxonomic identifier

343509 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Sodalis › Sodalis glossinidius

Protein attributes

Sequence length	281 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity. HAMAP-Rule MF_00817
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP-Rule MF_00817
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP-Rule MF_00817
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	queuosine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: HAMAP preQ1 synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 281	281	NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP-Rule MF_00817		PRO_0000247722

Sequences

	Sequence		Length	Mass (Da)
	Q2NRK0 [UniParc]. Last modified February 7, 2006. Version 1. Checksum: 65617DCC892E1497	FASTA	281	31,903
	10 20 30 40 50 60 MTNYDQHHAL QSLTLGKPTP YRERYDPSLL QAVPRALNRD LLGLQATALP FHGADIWTLY 70 80 90 100 110 120 ELSWLNDAGI PQVAIGEIAL NATSTNLIES KSFKLYLNSL NQTSFPSREA VRVRLAEDLS 130 140 150 160 170 180 RCADGEVQVV LRALSDFTSS PLLDFDGECI DDQPIHIDDY TFTNRYLEGA AGGSVVSETL 190 200 210 220 230 240 VSHLLKSNCL ITHQPDWGSV QIRYRGARID REALLRYLVS FRQHNEFHEQ CVERIFCDVM 250 260 270 280 QFCRPETLTV YARYTRRGGL DINPWRSNTH FSPATGRLAR Q

References

[1]

"Massive genome erosion and functional adaptations provide insights into the symbiotic lifestyle of Sodalis glossinidius in the tsetse host." Toh H., Weiss B.L., Perkin S.A.H., Yamashita A., Oshima K., Hattori M., Aksoy S. Genome Res. 16:149-156(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: morsitans.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP008232 Genomic DNA. Translation: BAE75225.1.
RefSeq	YP_455630.1. NC_007712.1.
3D structure databases
ProteinModelPortal	Q2NRK0.
SMR	Q2NRK0. Positions 21-281.
ModBase	Search...
Protein-protein interaction databases
STRING	343509.SG1950.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAE75225; BAE75225; ENAG00097246.
GeneID	3867344.
KEGG	sgl:SG1950.
PATRIC	23654867. VBISodGlo61428_4718.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0780.
KO	K06879.
OMA	ERIFCDL.
ProtClustDB	PRK11792.
Enzyme and pathway databases
BioCyc	SGLO343509:GJJC-2016-MONOMER.
UniPathway	UPA00392.
Family and domain databases
HAMAP	MF_00817. QueF_type2.
InterPro	IPR016428. CN_OxRdtase_NADPH-dep_YqcD. IPR020602. GTP_CycHdrlase_I/CN_OxRdtase. [Graphical view]
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMs	TIGR03138. QueF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUEF_SODGM

Accession

Primary (citable) accession number: Q2NRK0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	February 7, 2006
Last modified:	May 1, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program