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Q2NQI3 (PCKA_SODGM) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoenolpyruvate carboxykinase [ATP]

Short name=PCK
Short name=PEP carboxykinase
Short name=PEPCK
EC=4.1.1.49
Gene names
Name:pckA
Ordered Locus Names:SG2317
OrganismSodalis glossinidius (strain morsitans) [Complete proteome] [HAMAP]
Taxonomic identifier343509 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSodalis

Protein attributes

Sequence length539 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the gluconeogenesis. Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP) through direct phosphoryl transfer between the nucleoside triphosphate and OAA By similarity. HAMAP-Rule MF_00453

Catalytic activity

ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2. HAMAP-Rule MF_00453

Cofactor

Binds 1 manganese ion per subunit By similarity. HAMAP-Rule MF_00453

Pathway

Carbohydrate biosynthesis; gluconeogenesis. HAMAP-Rule MF_00453

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00453

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00453.

Sequence similarities

Belongs to the phosphoenolpyruvate carboxykinase [ATP] family.

Ontologies

Keywords
   Biological processGluconeogenesis
   Cellular componentCytoplasm
   LigandATP-binding
Manganese
Metal-binding
Nucleotide-binding
   Molecular functionDecarboxylase
Lyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processgluconeogenesis

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoenolpyruvate carboxykinase (ATP) activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 539539Phosphoenolpyruvate carboxykinase [ATP] HAMAP-Rule MF_00453
PRO_0000236947

Regions

Nucleotide binding247 – 2559ATP By similarity
Nucleotide binding448 – 4492ATP By similarity

Sites

Metal binding2121Manganese By similarity
Metal binding2311Manganese; via tele nitrogen By similarity
Metal binding2681Manganese By similarity
Binding site641Substrate By similarity
Binding site2061Substrate By similarity
Binding site2121ATP By similarity
Binding site2121Substrate By similarity
Binding site2311ATP By similarity
Binding site2961ATP By similarity
Binding site3321ATP By similarity
Binding site3321Substrate By similarity
Binding site4541ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2NQI3 [UniParc].

Last modified February 7, 2006. Version 1.
Checksum: 96FF0A35152F74CB

FASTA53959,267
        10         20         30         40         50         60 
MVSESITPQQ WISYGITGYR ELVYNPGYDQ LFAEEIDPAL SGFERGTLTQ SGAVAVDTGI 

        70         80         90        100        110        120 
FTGRSPFDKY VVRDDKTRDT LWWSDQGKGA NDNHPLDQQT WVHLKRRVGK QLSGKRLFVI 

       130        140        150        160        170        180 
DAFCGANEDS RLRVRFVTEV AWQAHFVKNM FIRPSDEELR DFEPDFVVLN GAKCTNPDWR 

       190        200        210        220        230        240 
EQGLHSENFV AFNLTEGIQL IGGTWYGGEM KKGLFSVMNY LLPLKGIASM HCSANVGTDD 

       250        260        270        280        290        300 
DVALFFGLSG TGKTTLSTDP RRRLIGDDEH GWDDDGVFNF EGGCYAKTIR LSPEAEPEIY 

       310        320        330        340        350        360 
QAIRRNALLE NVVVRADGSV DYDDGSKTEN ARVSYPIDHI DNIVKPVSRA GHATRVIFLT 

       370        380        390        400        410        420 
ADAFGVLPPV ASLSMEQAQY HFLSGFTAKL GGTERGVVAP VPTFSACFGA AFLSLHPTAY 

       430        440        450        460        470        480 
ADVLAKRMHA AGARAYLVNT GWNGSGKRIS LKDTRTIINA ILRSDIDDAP TATLPVFNLT 

       490        500        510        520        530 
IPTALPGVNG AILDPRTTYA SVDEWRAKAD DLAQRFIDNF SKFTDTPAGV ALVSAGPQR 

« Hide

References

[1]"Massive genome erosion and functional adaptations provide insights into the symbiotic lifestyle of Sodalis glossinidius in the tsetse host."
Toh H., Weiss B.L., Perkin S.A.H., Yamashita A., Oshima K., Hattori M., Aksoy S.
Genome Res. 16:149-156(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: morsitans.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP008232 Genomic DNA. Translation: BAE75592.1.
RefSeqYP_455997.1. NC_007712.1.

3D structure databases

ProteinModelPortalQ2NQI3.
SMRQ2NQI3. Positions 6-538.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING343509.SG2317.

Proteomic databases

PRIDEQ2NQI3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAE75592; BAE75592; SG2317.
GeneID3866323.
KEGGsgl:SG2317.
PATRIC23656701. VBISodGlo61428_5620.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1866.
HOGENOMHOG000271471.
KOK01610.
OMASKTENAR.
OrthoDBEOG6DG2RK.

Enzyme and pathway databases

BioCycSGLO343509:GJJC-2398-MONOMER.
UniPathwayUPA00138.

Family and domain databases

Gene3D3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPMF_00453. PEPCK_ATP.
InterProIPR001272. PEP_carboxykinase_ATP.
IPR013035. PEP_carboxykinase_C.
IPR008210. PEP_carboxykinase_N.
IPR015994. PEPCK_ATP_CS.
[Graphical view]
PfamPF01293. PEPCK_ATP. 1 hit.
[Graphical view]
PIRSFPIRSF006294. PEP_crbxkin. 1 hit.
SUPFAMSSF68923. SSF68923. 1 hit.
TIGRFAMsTIGR00224. pckA. 1 hit.
PROSITEPS00532. PEPCK_ATP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePCKA_SODGM
AccessionPrimary (citable) accession number: Q2NQI3
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: February 7, 2006
Last modified: May 14, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways