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Q2NL57

- UB10A_XENLA

UniProt

Q2NL57 - UB10A_XENLA

Protein

Ubiquitin carboxyl-terminal hydrolase 10-A

Gene

usp10-a

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 42 (01 Oct 2014)
      Sequence version 1 (07 Feb 2006)
      Previous versions | rss
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    Functioni

    Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/tp53. Acts as an essential regulator of p53/tp53 stability: in unstressed cells, specifically deubiquitinates p53/tp53 in the cytoplasm, leading to counteracts MDM2 action and stabilize p53/tp53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/tp53, leading to regulate the p53/TP53-dependent DNA damage response. Component of a regulatory loop that controls autophagy and p53/tp53 levels By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei417 – 4171NucleophilePROSITE-ProRule annotation
    Active sitei742 – 7421Proton acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. cysteine-type endopeptidase activity Source: UniProtKB
    2. ion channel binding Source: UniProtKB
    3. p53 binding Source: UniProtKB
    4. ubiquitin-specific protease activity Source: UniProtKB
    5. ubiquitin thiolesterase activity Source: UniProtKB

    GO - Biological processi

    1. autophagy Source: UniProtKB-KW
    2. DNA damage response, signal transduction by p53 class mediator Source: UniProtKB
    3. DNA repair Source: UniProtKB-KW
    4. protein deubiquitination Source: UniProtKB
    5. regulation of autophagy Source: UniProtKB
    6. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Autophagy, DNA damage, DNA repair, Ubl conjugation pathway

    Protein family/group databases

    MEROPSiC19.018.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 10-A (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 10-A
    Ubiquitin thioesterase 10-A
    Ubiquitin-specific-processing protease 10-A
    Gene namesi
    Name:usp10-a
    OrganismiXenopus laevis (African clawed frog)
    Taxonomic identifieri8355 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

    Organism-specific databases

    XenbaseiXB-GENE-966190. usp10.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. early endosome Source: UniProtKB
    3. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 791791Ubiquitin carboxyl-terminal hydrolase 10-APRO_0000393002Add
    BLAST

    Proteomic databases

    PRIDEiQ2NL57.

    Structurei

    3D structure databases

    ProteinModelPortaliQ2NL57.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini408 – 788381USPAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C19 family. USP10 subfamily.Curated
    Contains 1 USP domain.Curated

    Phylogenomic databases

    HOVERGENiHBG059823.
    KOiK11841.

    Family and domain databases

    InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    IPR028767. USP10.
    [Graphical view]
    PANTHERiPTHR24006:SF69. PTHR24006:SF69. 1 hit.
    PfamiPF00443. UCH. 1 hit.
    [Graphical view]
    PROSITEiPS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q2NL57-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASPSEQYIF GEFSDDEFKQ FFVTARCTVE LPPYNEHFFP CGPQSSGDFQ    50
    DGEECPRIEF GIEEVIDHNT TLPNNTDYSI SSNLNPQAPE FILTCSSSPK 100
    DSNNVLHENN FDAIDCQFSE SSIPDGSGNA DSDGTSGTGQ RERKKKKKRP 150
    PGYYSYLEGV GDVPSETLLN GHANSAGLNS ISTDDPDLAE DIPISTTSPR 200
    TCTSPDNFVD LNNEALSDDA SMHNVLDNTR TAGQPEECSV TSSEQSCIPS 250
    DNGRESPVRT AVVQPFAGTD TTENLGVTNG QTLESPEEDT ASNGVVLHPE 300
    VISLSEEAKA EEISTAQAVI HLPGSASANA PAKSWASLFH NSKPSSTPQV 350
    AYVETKNTPP VTSLQVTEKQ VEIKEGPVPV SEDPVAIKIA ELLEEVKLVH 400
    KPVSLQPRGL INKGNWCYIN ATLQALVACP PMYHLMKSIP VYTKAQKPCT 450
    STPMIDSFVR LMNEFTNMPI LPKAKQAPGE KVVRDIRPGA PFEPAYIYRL 500
    LTVFKSSLSE KGRQEDAEEY LGFILNGLHE EMLALKKLLL PQNDKIHINN 550
    GPDPVFATEE VNKEEQEGSD EEWEQVGPRN KSSVTRQADF VQTPITDIFG 600
    GHMRSVVYQQ SSKESATLQP FFTLQLDIQS EKIRTVQDAL ESLVARESVQ 650
    GYTTKTKQEV EICRRVTLEE LPPVLVLHLK RFVFEKTGGC QKLIKNIEYP 700
    VDLEISKDLL SPGVKSKIFK GQRTYRLFAV VYHHGNSATG GHYTTDVFQI 750
    GLNGWLRIDD QTVKVINQYQ VVKQTVERTA YLLYYRRVDL L 791
    Length:791
    Mass (Da):87,563
    Last modified:February 7, 2006 - v1
    Checksum:i650D8F74F5A047F9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC110953 mRNA. Translation: AAI10954.1.
    RefSeqiNP_001090293.1. NM_001096824.1.
    UniGeneiXl.45461.

    Genome annotation databases

    GeneIDi779202.
    KEGGixla:779202.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC110953 mRNA. Translation: AAI10954.1 .
    RefSeqi NP_001090293.1. NM_001096824.1.
    UniGenei Xl.45461.

    3D structure databases

    ProteinModelPortali Q2NL57.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C19.018.

    Proteomic databases

    PRIDEi Q2NL57.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 779202.
    KEGGi xla:779202.

    Organism-specific databases

    Xenbasei XB-GENE-966190. usp10.

    Phylogenomic databases

    HOVERGENi HBG059823.
    KOi K11841.

    Family and domain databases

    InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    IPR028767. USP10.
    [Graphical view ]
    PANTHERi PTHR24006:SF69. PTHR24006:SF69. 1 hit.
    Pfami PF00443. UCH. 1 hit.
    [Graphical view ]
    PROSITEi PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Xenopus Gene Collection (XGC) project
      Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Eye.

    Entry informationi

    Entry nameiUB10A_XENLA
    AccessioniPrimary (citable) accession number: Q2NL57
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 23, 2010
    Last sequence update: February 7, 2006
    Last modified: October 1, 2014
    This is version 42 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3