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Protein

Ubiquitin carboxyl-terminal hydrolase 10-A

Gene

usp10-a

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/tp53. Acts as an essential regulator of p53/tp53 stability: in unstressed cells, specifically deubiquitinates p53/tp53 in the cytoplasm, leading to counteracts MDM2 action and stabilize p53/tp53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/tp53, leading to regulate the p53/TP53-dependent DNA damage response. Component of a regulatory loop that controls autophagy and p53/tp53 levels (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei417 – 4171NucleophilePROSITE-ProRule annotation
Active sitei742 – 7421Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

  1. cysteine-type endopeptidase activity Source: UniProtKB
  2. ion channel binding Source: UniProtKB
  3. p53 binding Source: UniProtKB
  4. ubiquitin-specific protease activity Source: UniProtKB
  5. ubiquitin thiolesterase activity Source: UniProtKB

GO - Biological processi

  1. autophagy Source: UniProtKB-KW
  2. DNA damage response, signal transduction by p53 class mediator Source: UniProtKB
  3. DNA repair Source: UniProtKB-KW
  4. protein deubiquitination Source: UniProtKB
  5. regulation of autophagy Source: UniProtKB
  6. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Autophagy, DNA damage, DNA repair, Ubl conjugation pathway

Protein family/group databases

MEROPSiC19.018.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 10-A (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 10-A
Ubiquitin thioesterase 10-A
Ubiquitin-specific-processing protease 10-A
Gene namesi
Name:usp10-a
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-966190. usp10.

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. early endosome Source: UniProtKB
  3. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 791791Ubiquitin carboxyl-terminal hydrolase 10-APRO_0000393002Add
BLAST

Proteomic databases

PRIDEiQ2NL57.

Structurei

3D structure databases

ProteinModelPortaliQ2NL57.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini408 – 788381USPAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family. USP10 subfamily.Curated
Contains 1 USP domain.Curated

Phylogenomic databases

HOVERGENiHBG059823.
KOiK11841.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR028767. USP10.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PANTHERiPTHR24006:SF69. PTHR24006:SF69. 1 hit.
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2NL57-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASPSEQYIF GEFSDDEFKQ FFVTARCTVE LPPYNEHFFP CGPQSSGDFQ
60 70 80 90 100
DGEECPRIEF GIEEVIDHNT TLPNNTDYSI SSNLNPQAPE FILTCSSSPK
110 120 130 140 150
DSNNVLHENN FDAIDCQFSE SSIPDGSGNA DSDGTSGTGQ RERKKKKKRP
160 170 180 190 200
PGYYSYLEGV GDVPSETLLN GHANSAGLNS ISTDDPDLAE DIPISTTSPR
210 220 230 240 250
TCTSPDNFVD LNNEALSDDA SMHNVLDNTR TAGQPEECSV TSSEQSCIPS
260 270 280 290 300
DNGRESPVRT AVVQPFAGTD TTENLGVTNG QTLESPEEDT ASNGVVLHPE
310 320 330 340 350
VISLSEEAKA EEISTAQAVI HLPGSASANA PAKSWASLFH NSKPSSTPQV
360 370 380 390 400
AYVETKNTPP VTSLQVTEKQ VEIKEGPVPV SEDPVAIKIA ELLEEVKLVH
410 420 430 440 450
KPVSLQPRGL INKGNWCYIN ATLQALVACP PMYHLMKSIP VYTKAQKPCT
460 470 480 490 500
STPMIDSFVR LMNEFTNMPI LPKAKQAPGE KVVRDIRPGA PFEPAYIYRL
510 520 530 540 550
LTVFKSSLSE KGRQEDAEEY LGFILNGLHE EMLALKKLLL PQNDKIHINN
560 570 580 590 600
GPDPVFATEE VNKEEQEGSD EEWEQVGPRN KSSVTRQADF VQTPITDIFG
610 620 630 640 650
GHMRSVVYQQ SSKESATLQP FFTLQLDIQS EKIRTVQDAL ESLVARESVQ
660 670 680 690 700
GYTTKTKQEV EICRRVTLEE LPPVLVLHLK RFVFEKTGGC QKLIKNIEYP
710 720 730 740 750
VDLEISKDLL SPGVKSKIFK GQRTYRLFAV VYHHGNSATG GHYTTDVFQI
760 770 780 790
GLNGWLRIDD QTVKVINQYQ VVKQTVERTA YLLYYRRVDL L
Length:791
Mass (Da):87,563
Last modified:February 7, 2006 - v1
Checksum:i650D8F74F5A047F9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC110953 mRNA. Translation: AAI10954.1.
RefSeqiNP_001090293.1. NM_001096824.1.
UniGeneiXl.45461.

Genome annotation databases

GeneIDi779202.
KEGGixla:779202.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC110953 mRNA. Translation: AAI10954.1.
RefSeqiNP_001090293.1. NM_001096824.1.
UniGeneiXl.45461.

3D structure databases

ProteinModelPortaliQ2NL57.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiC19.018.

Proteomic databases

PRIDEiQ2NL57.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi779202.
KEGGixla:779202.

Organism-specific databases

XenbaseiXB-GENE-966190. usp10.

Phylogenomic databases

HOVERGENiHBG059823.
KOiK11841.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR028767. USP10.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PANTHERiPTHR24006:SF69. PTHR24006:SF69. 1 hit.
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Xenopus Gene Collection (XGC) project
    Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Eye.

Entry informationi

Entry nameiUB10A_XENLA
AccessioniPrimary (citable) accession number: Q2NL57
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 23, 2010
Last sequence update: February 7, 2006
Last modified: January 7, 2015
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.