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Protein

Centromere protein X

Gene

CENPX

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

DNA-binding component of the Fanconi anemia (FA) core complex. Required for the normal activation of the FA pathway, leading to monoubiquitination of the FANCI-FANCD2 complex in response to DNA damage, cellular resistance to DNA cross-linking drugs, and prevention of chromosomal breakage. In complex with CENPS (MHF heterodimer), crucial cofactor for FANCM in both binding and ATP-dependent remodeling of DNA. Stabilizes FANCM. In complex with CENPS and FANCM (but not other FANC proteins), rapidly recruited to blocked forks and promotes gene conversion at blocked replication forks. In complex with CENPS, CENPT and CENPW (CENP-T-W-S-X heterotetramer), involved in the formation of a functional kinetochore outer plate, which is essential for kinetochore-microtubule attachment and faithful mitotic progression. As a component of MHF and CENP-T-W-S-X complexes, binds DNA and bends it to form a nucleosome-like structure. DNA-binding function is fulfilled in the presence of CENPS, with the following preference for DNA substates: Holliday junction > double-stranded > splay arm > single-stranded. Does not bind DNA on its own.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processCell cycle, Cell division, DNA damage, DNA repair, Mitosis

Enzyme and pathway databases

ReactomeiR-BTA-606279 Deposition of new CENPA-containing nucleosomes at the centromere
R-BTA-6783310 Fanconi Anemia Pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Centromere protein X
Short name:
CENP-X
Alternative name(s):
FANCM-interacting histone fold protein 2
Fanconi anemia-associated polypeptide of 10 kDa
Stimulated by retinoic acid gene 13 protein homolog
Gene namesi
Name:CENPX
Synonyms:FAAP10, MHF2, STRA13
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 19

Organism-specific databases

VGNCiVGNC:27188 CENPX

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003371791 – 79Centromere protein XAdd BLAST79

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ2NKU0

Expressioni

Gene expression databases

BgeeiENSBTAG00000004108

Interactioni

Subunit structurei

Heterodimer with CENPX, sometimes called MHF; this interaction stabilizes both partners. MHF heterodimers can assemble to form tetrameric structures. MHF also coassemble with CENPT-CENPW heterodimers at centromeres to form the tetrameric CENP-T-W-S-X complex. Forms a discrete complex with FANCM and CENPX, called FANCM-MHF; this interaction, probably mediated by direct binding between CENPS and FANCM, leads to synergistic activation of double-stranded DNA binding and strongly stimulates FANCM-mediated DNA remodeling. Recruited by FANCM to the Fanconi anemia (FA) core complex, which consists of CENPS, CENPX, FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL, FANCM, FAAP24 and FAAP100. The FA core complex associates with Bloom syndrome (BLM) complex, which consists of at least BLM, DNA topoisomerase 3-alpha (TOP3A), RMI1/BLAP75, RPA1/RPA70 and RPA2/RPA32. The super complex between FA and BLM is called BRAFT.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000005369

Structurei

3D structure databases

SMRiQ2NKU0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CENP-X/MHF2 family.Curated

Phylogenomic databases

eggNOGiENOG410J30M Eukaryota
ENOG410XYKN LUCA
GeneTreeiENSGT00390000002725
HOGENOMiHOG000231242
InParanoidiQ2NKU0
KOiK15360
OMAiLLMREML
OrthoDBiEOG091G1595
TreeFamiTF330764

Family and domain databases

InterProiView protein in InterPro
IPR018552 CENP-X
PANTHERiPTHR28680 PTHR28680, 1 hit
PfamiView protein in Pfam
PF09415 CENP-X, 1 hit

Sequencei

Sequence statusi: Complete.

Q2NKU0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEETGAVFRK ELVSKLLHLH FKDKKTKVSG DALQLVAELL KIFVVEAAIR
60 70
SVRQAQAEGL AHVDVEQLEK VLPQLLLDF
Length:79
Mass (Da):8,879
Last modified:February 7, 2006 - v1
Checksum:i34E131AAF52AA041
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC111642 mRNA Translation: AAI11643.1
RefSeqiNP_001107999.1, NM_001114527.2
UniGeneiBt.15739

Genome annotation databases

EnsembliENSBTAT00000005369; ENSBTAP00000005369; ENSBTAG00000004108
GeneIDi618018
KEGGibta:618018

Similar proteinsi

Entry informationi

Entry nameiCENPX_BOVIN
AccessioniPrimary (citable) accession number: Q2NKU0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: February 7, 2006
Last modified: March 28, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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